Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"glucose-6-phosphate dehydrogenase"”.
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Regulation of glucose -6 -phosphate dehydrogenase by polyunsaturated fatty acids in cultured rat hepatocytes
… we are using to study this phenomenon is liver glucose-6-phosphate dehydrogenase (G6PD). In order to determine if regulation of G6PD occurred at a transcriptional or a posttranscriptional step in rat hepatocytes, enzyme activity, mRNA accumulation and transcriptional activity of the G6PD gene …
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The actions of gossypol on the physiologic antioxidant defense system
… Catalase, glutathione peroxidase, ascorbate and glucose-6-phosphate dehydrogenase were decreased in the testis. In contrast, antioxidants including catalase and glutathione reductase were increased in the hearts of gossypol treated rats. The selective inhibition of testis and hepatic antioxidants …
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"Isozyme variation in ""helminthosporium-type"" species pathogenic on maize"
… (EC 2.6.1.1), fumarase (EC 4.2.1.2), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), and mannose-6-phosphate isomerase (EC 5.3.1.8). Associated electrophoretic and virulence phenotypes were found, indicating that populations exist in S. turcica as asexually propagated clonal groups. …
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A taxonomic study of the genus Spirillum ehrenberg, with special reference to nutrition and carbohydrate catabolism
… and the effects of various inhibitors on glucose-6-phosphate dehydrogenase activity from P. lunatum. The resultant data, plus that of Wells (1970) and McElroy (1970), have been used to divide genus Spirillum into four genera (Aquaspirillum, Spirillum, Oceanospirillum, and Pseudospirillum), …
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DESIGN AND INVESTIGATION OF DUMBBELL-SHAPED DNA MINIMAL VECTORS FOR CRISPR/CAS9 EDITING
… CRISPR/Cas9 system. Using three targets, i.e. glucose-6-phosphate dehydrogenase (G6PD) Mahidol, G6PD Mediterranean and Southeast Asian Ovalocytosis, and a dual reporter assay, we demonstrated that dumbbell vectors offer a viable alternative to plasmids for delivery of the CRISPR/Cas9 gene …
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Electrostatic and affinity enhancements of protein partitioning in two-phase aqueous micellar systems
… partitioning of the enzyme glucose-6-phosphate dehydrogenase (G6PD) in two-phase aqueous mixed (nonionic/cationic) micellar systems was investigated experimentally and theoretically. The successful enhancement, up to 22-fold, of the partitioning of the negatively-charged G6PD …
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Temperature Acclimation of Enzyme Activities and Protein Synthesis in Channel Catfish (Ictalurus Punctatus). In Vivo and In Vitro Studies
… NADH-cytochrome(DEGREES)C reductase, and glucose-6-phosphate dehydrogenase. Lactate dehydrogenase showed no compensation. 6-phosphogluconate dehydrogenase showed partial inverse compensation. Hence, cultured hepatocytes show temperature acclimation of enzymes in patterns similar to those …
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Molecular, genetic, and biochemical studies of mannityl opine utilization by Agrobacterium tumefaciens: Its evolutionary implications
… homologues of 6-phosphogluconate dehydratase and glucose-6-phosphate dehydrogenase, respectively.
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Effects of acute and chronic exercise on the activities of hepatic lipogenic enzymes in rats
… fed a high-fructose diet than in rats fed high-glucose or high-cornstarch diets. Exercise decreased CL, PK, FAS, and glucose-6-phosphate dehydrogenase (G6PDH) activities, especially in fructose-fed rats. Study 2 examined the time course of dietary induction of enzymes in fasted/refed rats, and …
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Incidence, aetiology and short term outcomes of extreme hyperbilirubinaemia, in term infants born in the West Metro area of Cape Town, South Africa between 2019 and 2020
… ABO incompatibility (31/51, 61%), followed by glucose-6-phosphate dehydrogenase deficiency (11/51, 22%). Twelve infants (20 %) underwent an exchange transfusion. Six infants were encephalopathic. Forty-seven infants (80%) were readmitted after initial post-natal discharge, with a mean age of …
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The effects of cellular quiescence on the antioxidant defense enzymes of bovine embryonic lung fibroblasts: a survey
… reductase, glutathione peroxidase, and glucose-6-phosphate dehydrogenase, was studied. We have also extensively monitored the levels of free radical in the cell by both direct and indirect methods. The results indicate that no significant (p<0.05) changes in the activity of any of the …
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The Economics of Vivax Malaria Treatment
… on the Thai-Myanmar border, where screening for glucose-6-phosphate-dehydrogenase (G6PD) deficiency prior to radical cure reduced the disease burden while being a cost-effective option for healthcare providers that do not use radical cure and a potentially cost-saving alternative where primaquine …
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Purification of Calmodulin and Quantification of Its Levels in Mammary Tissue and Isolated Cells During Pregnancy and Lactation (Acini, Secretory, Myoepithelial)
… lactation and involution. Protein, DNA, lactose, glucose-6-phosphate dehydrogenase and alkaline phosphatase were assayed to characterize the cell fractions. By using RIA, the calmodulin content per mg of protein in isolated secretory cells was high near parturition, then decreased and remained …
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… thermostable enzymes. In this project, ribose-5-phosphate isomerase (RpiB), one of the most important enzymes in the pentose phosphate pathway, was cloned from a thermophile Thermotoga maritima, and heterologously expressed in Escherichia coli, purified and characterized. High-purity RpiB was …
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Colorado cytospora canker complex on Populus tremuloides Michx.
… only three, viz., alpha esterase, amylase, and glucose-6-phosphate dehydrogenase, provided good resolution for all isolates. Following cluster analysis, two major clades well-delineated the two taxa. Phylogenetic analyses of ITS1-5.8S-ITS2 rDNA and EF-1α sequences produced phylogenetic trees in …
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Auxiliary metabolic genes in viruses infecting marine cyanobacteria
… genes involved in photosynthesis and the pentose phosphate pathway (PPP). The overarching goal of this work is to understand the selective pressures driving the acquisition and maintenance of these particular AMGs by cyanophage. Transaldolase is thought to be a key step in the host PPP. The …
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Coenzyme engineering of NAD(P)+ dependent dehydrogenases
… form--nicotinamide adenine dinucleotide phosphate (NADP, including NADP+ and NADPH) are two of the most important biological electron carriers. Most NAD(P) dependent redox enzymes show a preference of either NADP or NAD as an electron acceptor or donor depending on their unique metabolic …
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Biochemical and Molecular Assessment of Toxicity of Primaquine Metabolites on Erythrocytes
… due to its hemolytic toxicity, especially in glucose-6-phosphate dehydrogenase deficient (G6PDd) individuals. Phenolic and quinone metabolites generated via cytochrome P450-dependent pathways appear to be responsible for hemolytic effects of PQ. However, the mechanism for the hemolytic …
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