Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"glucose stimulation"”.
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Molecular Adaptation of Pancreatic Beta-Cell Function During Endoplasmic Reticulum Stress
Pancreatic beta-cells may balance glucose-stimulated insulin synthesis and secretion with the proteasome activity to regulate protein concentrations in the endoplasmic reticulum (ER). During this process, reactive oxygen species (ROS) may originate as byproducts from the oxidative protein folding …
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Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.
… insulin secretion, and especially what couples glucose stimulation of the beta-cell to insulin secretion. We have found that anaplerosis via pyruvate carboxylase is essential for both phases of glucose-stimulated insulin secretion, presumably via generation of an increased ATP/ADP ratio. …
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Quantification of gene expression in single cells
… mRNA levels were increased in response to glucose stimulation; an effect due to an increased fraction of cells with high expression. The insulin genes Ins1 and Ins2 have similar promoter regions and were indeed co regulated within single beta-cells. Na-channels in alpha- and beta-cells …
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The use of human pluripotent stem cells to model HNF1B-associated diabetes
… as well as impaired insulin secretion upon glucose stimulation. Cell proliferation analyses showed a significant decrease in the proliferation rate in HNF1B heterozygous and homozygous mutant cells compared with wild-type cells at the foregut stage while no change in the apoptosis rate could …
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Small gtp-binding proteins in insulin secretion
… Understanding the molecular events leading to Glucose-stimulated insulin secretion [GSIS] might serve as therapeutic potential towards diabetes. GSIS involves interplay between small G-proteins and their regulatory factors. Herein, I tested the hypothesis that Arf nucleotide binding site opener …
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Broad Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or β-cell Metabolism in the Absence of Dietary Overnutrition
… indistinguishable insulin secretion responses to glucose or pyruvate, and exhibited no differences in function or viability in response to metabolic or ER stress-inducing agents. Moreover, metabolomic profiling revealed that, when compared to SIRT3-null cell lines, expression of wild-type SIRT3 …
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Role of PARK2Gene in Maintenance of Pancreatic Beta Cell Functions
… (SNPs) in the 28 mitophagy-related genes with glucose- and insulin-related quantitative traits in the Korean study cohort (7,551 subjects), I found that two SNPs, rs10455889 and rs9365294, in the PARK2 gene encoding an E3 ubiquitin-protein ligase (parkin) were significantly associated with …
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Cell Biology of the ICA69 protein family in Neurosecretory cells
… machinery of beta-cells, ICA69-RP appeared to be glucose regulated, as shown by a 1.55 fold increase in mRNA levels upon stimulation of the cells with 25 mM glucose for two hours.Glucose stimulation of beta-cells prompts the activation of post-transcripional mechanisms which quickly up-regulate …
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IL-1β Amplification of Nitric Oxide Production and Its Inhibitory Effects on Glucose Induced Early Growth Response-1 Expression in INS-1 Cells
… in pancreatic beta cells in response to glucose. We hypothesized that interleukin-1 beta (IL-1▀) induction of nitric oxide alters glucose induced Egr-1 transcription levels. Egr-1 levels were assessed via western blot, nitric oxide was measured with a Griess Reagent kit and insulin levels …