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Showing 1 to 14 of 14 for “"glucose repression"”.
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Design of a Saccharomyces cerevisiae strain capable of simultaneously utilizing cellobiose and xylose
… production from lignocelluloses. However, glucose repression limits efficient ethanol production because glucose in lignocellulosic hydrolysates inhibits xylose and other sugars’ utilization. As a result, it is attractive to construct a glucose derepressed S. cerevisiae strain for efficient …
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Characterization of an ethanologenic yeast inhibiting atypical galactose metabolism
… delayed with respect to other sugars, such as glucose and mannose, because of an intrinsic preference for carbon sources requiring less energy in the preparatory reactions preceding glycolysis. This work comprised the search for, and characterization of anethanologenic yeast capable of …
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Molecular cloning of three genes encoding SNF1/AMPK protein kinases from Drosophila melanogaster.
… genes are known to play a role in the yeast glucose repression pathway and the SNF1 gene, encoding a serine-threonine protein kinase, has the essential function of releasing genes from glucose repression in response to glucose starvation. SNF1 homologues have been found in a number of …
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Improving advanced biofuels production in Saccharomyces cerevisiae via protein engineering and synthetic biology approaches
… utilization of sugar mixtures caused by glucose repression and the low efficiency of synthesizing fatty acid derived advanced biofuels. In this thesis, we aim to improve the production of advanced biofuels in S. cerevisiae using protein engineering and synthetic biology approaches. To …
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Strain engineering and biosensor development for efficient biofuel production by Saccharomyces cerevisiae
… S. cerevisiae strains capable of converting glucose or pentose sugars into ethanol. Unfortunately, pentose sugars, which constitute up to 30% of biomass hydrolysate, cannot be co-utilized simultaneously with glucose by recombinant S. cerevisiae strains. Great efforts have been made to improve …
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… that cytochrome b$\sb{562}$ expression is under glucose repression. A high level expression system for cytochrome b$\sb{562}$ was constructed where the protein expression is driven from the lac-promotor located on pUC18. The level of expression of cytochrome b$\sb{562}$ in this system is 3-5% of …
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Toxicological studies in yeast species : production of cytochrome P450 and evaluation of carbon tetrachloride toxicity
… was optimally produced under conditions of glucose repression during the late logarithmic phase of growth. Levels of the enzyme were consistently found to be greater in respiratory deficient (petite) mutants of the yeast S. cerevisiae than in wild type strains. The enzyme was isolated from …
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The influence of magnesium ions on the growth and metabolism of <i>Saccharomyces cerevisiae</i>
… under conditions of micro-aerophillic glucose-repression, was shown to be hyperbolic, thus indicating a Monod relationship. The maximum growth rate was found to be 0.20hrs<sup>-1</sup> whilst K<sub>s</sub> was equal to 36μM and the yield co-efficient at 1/2μ<sub>max</sub> equal to 1.9 …
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Regulation and switching in bacterial gene expression networks in response to nutrients
… growth and easier accessibility. Catabolite repression refers to the process where the metabolism of one carbon source represses the genes involved in metabolizing another carbon source. The most classic example is glucose repression, which is investigated by many studies, but much less is …
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Engineering diverse yeast species for optimal sugar consumption and enhanced product formation
DSpace SAF Submission Ingestion Package generated from Vireo submission #9570 on 2016-07-07 at 14:18:10
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Räumliche und zeitliche Untersuchungen zum Teilungsapparat der äußeren Mitochondrienmembran mit hochauflösender Fluoreszenzmikroskopie
… konnte gezeigt werden, dass unter Glucoserepression in Δfis1-Mutanten ebenso wie in Wildtypzellen aus hochverzweigten Mitochondrien einfache überwiegend tubuläre Strukturen entstehen. In beiden Fällen sinkt dabei die Frequenz von Matrixseparationen und Fusionen. Doppelmarkierungen …
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The Regulation of NAP4 in Saccharomyces cerevisiae
<p>The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be …
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Functional and evolutionary analysis of sugar transporters in Kluyveromyces marxianus
… including thermotolerance, stress tolerance, glucose repression and sugar utilisation. While several K. marxianus genome sequences are currently available, a comparative analysis aiming to determine the level of genetic diversity between these sequences had not previously been performed. To …
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Metabolic engineering of yeast strains for renewable biomass utilization and valuable chemical production
… ethanol yield are usually lower compared to glucose. While screening various gene targets related glucose sensing, regulation and metabolism, TPS1 deletion expressed with mutant HXK2 allowed enhanced glucose or galactose fermentation. Because HXK2 also has a role in glucose signaling and …