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Showing 1 to 17 of 17 for “"glucose dehydrogenase"”.
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Preparation of a self-contained NADH co-factor recycling particle system
… activity, specificity and selectivity. Since glucose dehydrogenase (GDH) and NADH oxidase, are industrially relevant co-factor recycling enzymes for NAD(P)H and NAD+ respectively, characterisation of their immobilisation is of interest. The current work describes the use of the proprietary …
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FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES
… biocatalyst combining an ene reductase and a glucose dehydrogenase was developed for the selec:ve bioreduc:on of cinnamaldehyde, achieving high conversion and opera:onal stability under con:nuous flow, with in situ NADH regenera:on. Overall, this research provides new insights into the …
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The Role for Proteoglycans in Regulating Embryonic Patterning and Morphogenesis
… to mediate proteoglycan-ligand interactions. UDP-glucose dehydrogenase (Ugdh) is an enzyme required for the biosynthesis of GAGs, and in its absence the cell cannot polymerize sidechains. Drosophila mutant embryos deficient for Ugdh activity exhibit abnormal Fgf, Wnt, and TGFĂ signalling and die …
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Auswirkungen der Deletion membranständiger Dehydrogenasen auf Gluconobacter oxydans DSM 7145
… Die für die Oxidation verantwortlichen Dehydrogenasen werden in zwei Gruppen, membranständige und cytoplasmatische Dehydrogenasen, die sich in ihrer zellulären Lokalisation, ihrer Funktion in der Zelle und der Substratspezifität unterscheiden, unterteilt. Membranständige Dehydrogenasen …
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Engineering of an efficient and enantioselective biocatalyst for the preparation of chiral pharmaceutical intermediates
… recycling was developed by co-expressing a glucose dehydrogenase from Bacillus substilis or a phosphite dehydrogenase from Pseudomonas stutzeri together with the P450pyr system in a recombinant Escherichia coli.
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Growth and metabolism of Aquaspirillum gracile
… has not been previously investigated. Only D-glucose, D-galactose, and L-arabinose are attacked. The soluble portion of extracts of glucose-grown cells was found by spectrophotometric methods to contain enzyme activities characteristic of the Entner-Doudoroff and the Embden-Meyerhof-Parnas …
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Characterization of plasmids in Gluconobacter
… are well known for their plasma membrane-bound dehydrogenases that rapidly oxidize compounds and release the products into the medium. In 1989, Qazi et al. proposed that genes coding for membranebound glucose dehydrogenase are on a plasmid in G. oxydans strain ATCC 9937. The only other known …
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Factors preventing the metabolism of carbohydrates by Bacillus sphaericus 2362
… were undetected in cell extracts. In addition, glucose dehydrogenase activity was not detected. The inability to form glucose-6-phosphate from glucose prevents the catabolism of this and related substrates via the Entner-Doudoroff (ED), hexose monophosphate (HMP), and EMF pathways. Oxygen uptake …
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Biomarkers of Optic Nerve Head Glial Cell Activation Following Biomechanical Insult
… were astrocytic phosphoprotein (PEA15), UDP-glucose dehydrogenase (UGDH), and annexin A4 (ANXA4). LC proteins were bcl-2-associated athanogene 5 (BAG5), nucleolar protein 66 (NO66) and Eukaryotic translation initiation factor 5A (eIF-5A). These proteomic results will enable a series of …
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Prozessentwicklung mit maßgeschneiderten rekombinanten Biokatalysatoren zur Synthese einer Bulk- und einer Feinchemikalie
… oxidation of cyclohexanol by means of an alcohol dehydrogenase and a cyclohexanone monooxygenase towards ε-caprolactone without isolation of the intermediate cyclohexanone, inhibition of the monooxygenase especially by the product was addressed by solvent engineering. However, increase of …
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Cytochrome c-DNA and cytochrome c-enzyme interactions for the construction of analytical signal chains
… enzyme types, the flavin dependent fructose dehydrogenase (FDH) and the pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH), were tested as reaction partners of freely diffusing cyt c and cyt c immobilized on electrodes in mono- and MLs. The characterisation of the ET processes …
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Analysis of phenolic compounds by dint of GDH-biosensors and immunoassays
… zu realisieren. In diesem Fall wurde Glucose-Dehydrogenase (GDH) verwendet, die oxidierte phenolische Verbindungen reduzieren kann. Zusammen mit der Oxidation an der Sensoroberfläche bildet sich ein Verstärkungszyklus aus, der das ursprüngliche Signal vielfach erhöht. Wir waren in der …
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Development and use of a synthetic biology toolkit to enhance high value lipid production in Nannochloropsis oculata
… genome. Using the Cas9-based vector, the UDP-6-glucose dehydrogenase (UDGH) was targeted for HDR gene editing as a route to divert cellular resources away from cell wall synthesis to lipid biosynthesis and enhanced EPA production. Despite this, CRISPR-Cas9 HDR targeting vectors only yielded …
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Highly sensitive measurements of substrates and inhibitors on the basis of tyrosinase sensors and recycling systems
… Sauerstoffelektrode, ergänzt durch ein Enzym, Glucose zu messen, war die Entwicklung neuer Messtechnik nicht mehr aufzuhalten. Biosensoren, spezielle Messfühler, die aus einer Kombination aus biologischer Komponente (erlaubt eine spezifische Erkennung des Analyten auch ohne vorherige Reinigung …
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Genome Sequence Analysis and Characterization of Recombinant Enzymes from the Thermoacidophilic Archaeon Picrophilus torridus
… Enzyme, eine α-Glucosidase (MalP) und eine Glucose-1-Dehydrogenase (GdhA), wurden produziert, aufgereinigt und biochemisch charakterisiert.
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GLUCOSE ELECTROOXIDATION
The electrooxidation of glucose has attracted a lot of interest due to its applications in blood glucose sensors and biological fuel cells. Glucose sensors optimization is highly necessary to improve the treatment of Diabetes Mellitus, a chronic disease affecting millions of people around the …