Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"glucokinase"”.
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Application of simulation methods for the identification of allosteric binding site in human glucokinase
… have been applied to an allosteric enzyme human glucokinase (GLK) for which known allosteric activators have been discovered, as a test-case. First an X-ray structure, bound to glucose and an allosteric activator, has been examined as a benchmark. The apo form of the enzyme has also been studied …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… to have a thermophilic polyphosphate glucokinase. T. fusca YX ORF Tfu_1811 encoding a putative PPGK was cloned and the recombinant protein fused with a family 3 cellulose-binding module (CBM-PPGK) was over expressed in Escherichia coli. By a simple one-step immobilization, the …
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The molecular genetics of Type 2 diabetes
… with particular emphasis on the role of the glucokinase gene. Other work studied specific mutations in the tRNA<sup>Leu(UUR)</sup> gene of mitochondrial DNA and in the glucagon receptor gene. <br></br><br></br> The Introductory chapter presents an overview of Type 2 diabetes, and the …
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Hypothalamic Nutrient Sensing
… neurons. A subpopulation of AgRP neurons express glucokinase, and some AgRP neurons are glucose inhibited, but the role of glucokinase in these neurons has not been characterised. Our lab generated an AgRP neuron specific glucokinase knock out mouse line. Preliminary results suggest 18 – 25 week …
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Development of glucose valves for metabolic engineering applications in E. coli
… of glucose flux from central metabolism via glucokinase. Using a promoter library, glucokinase expression was varied with an attendant change in specific growth rate and carbon flux. A model pathway was then constructed to utilize the redirected carbon demonstrating that the efficiency of …
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Mechanism of Insulin Release From Islets of Langerhans in a Rat Model of Normal Infants and Infants of Diabetic Mothers
… and protein expression of GLUT1, GLUT2, and glucokinase by immunocytochemistry in the isolated islet and Western blot on the whole pancreas. The IDM had significantly lower mean body weights at all ages studied (p $<$ 0.01, n $\ge$ 34); significantly higher mean plasma glucose concentration …
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Examination of Glucose Transport in Clostridium Beijerinckii NCIMB 8052 and the Solvent Hyper-Producing Mutant BA101
… presence of ATP, consistent with the presence of glucokinase activity in C. beijerinckii extracts. ATP-dependent glucose phosphorylation was predominant during the solventogenic stage when PEP-dependent glucose phosphorylation was dramatically repressed. A nearly two-fold greater ATP-dependent …
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General method for the synthesis of pseudodisaccharides. Diels-Alder approach to the synthesis of pseudodisaccharides
… of inhibitors of Golgi ¿-mannosidase II and glucokinase. The first chapter is a comprehensive survey of the reported synthetic routes to pseudodisaccharides from the literature. The results and discussions are presented in chapter 2. This chapter starts by discussion of the preparation of …
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Control of glucose and xylose utilization by Clostridium thermohydrosulfuricum Rt8.B1
… was carried out by a constitutive ATP-dependent glucokinase and that of xylose by an inducible ATP-dependent xylulokinase following isomerization by xylose isomerase. These enzymes not only initiated the metabolism of glucose and xylose in Cl. thermohydrosulfuricum Rt8.B1, but also served to …
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The antidiabetic properties of four plants grown in India and KwaZulu-Natal, South Africa, suitable for diabetic management
… the activity of Na/K ATPase and by assessing glucokinase and hexokinase activity by analysing liver and gastrocnemius muscle tissue. Results revealed that all four plants were reported to have protein, fats, glucose and starch, with the South African varieties showing higher protein content …
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Genetic and Molecular Analysis of Islet-and Liver-Enriched Transcription Factors in Metabolism and Development
… In addition, genetic defects in the glucokinase gene, the glucose sensor of the pancreatic β-cells, and the insulin gene also lead to impaired glucose tolerance. Using molecular and genetic approaches, we demonstrated that the MODY genes are functionally related and form an integrated …
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Growth and metabolism of Aquaspirillum gracile
… sugar. Glucose-grown cells contained not only glucokinase and glucose-6- p dehydrogenase activities but also glucose dehydrogenase activity. Gluconate dehydrogenase activity was not detected but gluconate kinase activity was present, Paper chromatographic analysis of the spent culture …
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The Art of Modeling and Simulation of Multiscale Biochemical Systems
… secretion can be reinstated by increasing the glucokinase level. This new discovery sheds light on antidiabetic medication. In order to study the stochastic dynamics of the insulin secretion pathway, we first apply the hybrid method to model the discrete events in the insulin secretion pathway. …
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Factors preventing the metabolism of carbohydrates by Bacillus sphaericus 2362
… detected in cell extracts. These enzymes were: glucokinase, hexokinase, beta-galactosidase, and amylase. Growth of this bacterium on glycerol as a sole source of carbon implies the presence of the enzymes from the lower half of the Embden-Meyerhof-Parnas (EMP) pathway. Two enzymes of the upper …
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Beyond the allosteric role of Fructose-2,6-bisphosphate: exploring its effects on gene expression and signal transduction.
… metabolism, raising F26BP levels stimulates glucokinase gene expression and inhibits glucose-6-phosphatase gene expression, thus, also favoring enhanced glycolysis. These effects are not limited to glucose metabolism, as raising F26BP levels in type 2 diabetic mice leads to decreased weight …
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Brain Insulin Action Regulates Hypothalamic Glucose Sensing and the Counterregulatory Response to Hypoglycemia
… including glucose transporters: GLUTs) and glucokinase: GK), and measuring regional brain glucose utilization. Understanding how the brain regulates the counterregulatory response to hypoglycemia is critical to devise therapies to combat severe hypoglycemia in diabetic patients. Overall, …
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Pyruvate Cycling Pathways and Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells
… and ATP:ADP, due to an unexpected decrease in glucokinase protein. This work questions the use of ACC inhibitors in obesity and diabetes therapy. In summary, these studies on ICDc, MEc, and ACC1, coupled with concurrent work in our laboratory, eliminate two potential pyruvate cycling pathways …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… will require manipulation of the hexokinase and glucokinase enzymes. Intraperitoneal implantation of the manipulated AtT20 cells into athymic nude mice with streptozotocin-induced diabetes resulted in decreased plasma glucose concentrations. The cells formed vascularised tumours in vivo which …
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Independent and interacting effects of diet and genetic risk on obesity-related comorbidities
… associated with changes in TC and HDL-C. The glucokinase regulator rs1260326 SNP was associated with TC changes, by which individuals in the avocado group experienced differential changes by presence or absence of the risk allele. Conclusions: SFA-UFA replacement may not be effective in adults …
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