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Showing 1 to 15 of 15 for “"glucanases"”.
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Characterization of structure and enzymic activity of endo-l, 4-β-D-glucanases purified from Trichoderma viride
Four electrophoretically distinct endo-1,4-β-D-glucanases have been isolated from a commercial Trichoderma viride enzyme preparation, Pancellase SS. These endoglucanases were distinguished by their affinity for microcrystalline cellulose; two were adsorbed onto a cellulose column at pH 5.0 …
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Purification and characterization of an endo-1,4-{u03B2}-D-glucanase and two exo-1,4-{u03B2}-D-glucanases from the cellulase system of Trichoderma reesei
… (E.C. 3.2.1.4) and two exo-1,4-β-D-glucanases (exo-cellobiohydrolases I (D) and II, E.C. 3.2.1.91) have been purified to electrophoretic homogeneity from the extracellular culture filtrate of the imperfect fungus Trichoderma reesei QM 9414 grown on microcrystalline cellulose …
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Genomic and Proteomic Characterisation of the European House Dust Mite, Dermatophagoides pteronyssinus
… allergens. The genes encoding novel β-1,3 glucanases were identified within a trigene cluster in D. pteronyssinus airmid. Recombinant protein expression, biochemical and proteomic analysis revealed Glu1 and Glu2 to exhibit hydrolytic activity toward β-1,3 glucans and have increased …
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Characterization of an alkalophilic Bacillus brevis isolate with respect to its endo-(1,3-1,4)-β-glucanase gene, protein hyperproduction and the degS-degU operon
… published data for Bacillus endo-(1,3-1,4)-β-glucanases. The enzyme exhibited some unique properties. The optimum temperature and pH for enzyme activity were 65-70°C and 8-10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be 29 …
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Characterization of endoglucanase cela from the rumen bacterium Clostridium longisporum
… This gene showed homology with other endo-B-1,4-glucanases from family 5 (Henrissat & Bairoch, 1993). Plasmid pCM64 was found to contain the whole celA gene encoding endoglucanase CelA, while pCM4 has a 5'-truncated gene, termed celM5', which encodes a fusion protein, CelMN', that was initiated …
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Screening and evaluation of bacteria for the biological control of Botrytis cinerea on Vitis vinifera
… testing for cell wall degrading enzymes (glucanases, proteases, chitinases), evaluating competition for space and nutrients via siderophore production on chrome-azurol S agar, and conducting an in vivo assay on wounded grape berries. Antibiosis was evaluated by assessing the effect of the …
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Optimization of sequential purification of beta-glucosidase from tricoderma reesei in aqueous two-phase system
… resulted in the precipitation of endo and exo-glucanases. During this separation step, beta-glucosidase activity was completely recovered in the supematant. The enzyme was further purified from other proteins by partitioning in aqueous two-phase systems. Preliminary investigation with pure …
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Exogenous enzymes and irradiation of barley reduce the anti-nutritional activity of non-starch polysaccharides in broilers
… consisting of cellulases, xylanases and â - glucanases (Roxazyme G at 150 g/ton). The two control diets were based on maize (low NSP diets) with either soy oil or yellow grease. The high NSP diets had significantly lower (P<0.05) apparent metabolisable energy (AME) and lipid digestibility …
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Analyses of a genic male-sterile mutant in soybean
… to linkage group K. Twelve classes of [beta]-1,3-glucanases were cloned and sequenced. Members of 11 classes mapped at 34 loci on 5 different linkage groups using an F2 population of 56 individuals. Northern blot analyses performed on total RNA from root, stem, leaf, pod, flower bud, and …
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Molecular analysis of migratory plant-parasitic nematodes with a focus on plant cell wall modifying enzymes
… on earth. In nematodes, cellulases or endo-1,4-β-glucanases which can degrade cellulose were mainly identified in sedentary species. A large part of this thesis is about the characterization of endoglucanases in migratory endoparasitic nematodes, namely in the species Radopholus similis, …
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Influência da concentração de biomassa na produção de etanol celulósico e caracterização de uma β-glucanase de kluyveromyces marxianus
… obtidas viimostraram alta similaridade com β-1,3 glucanases fúngicas. Além disto, foi medida a atividade hidrolitica desta enzima sobre a parede celular de leveduras. Os resultados mostram que a enzima possui um potencial para aplicação na produção de β-glucanos solúveis ou para preparação de …
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Plant cell wall degrading enzymes and their potential application to biofuel production
… hypothesized that Cb486 incubated with endoglucanases or endoxylanases should be able to enhance the release of glucose or xylose from cellulose and xylans respectively. As expected, Cb486 was also able to work synergistically with a number of endo-glucanases from the genome of C. bescii to …
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Soybean mosaic virus-soybean interactions: molecular, biochemical, physiological, and immunological analysis of resistance responses of soybean to soybean mosaic virus
… The SPR2 group was identified as β-1,3-glucanases (GLN) and classed two subgroups. The SPR3 group was identified as chitinases (CHN) and classed three distinct subgroups. Like HR, the N response induced a series of defense responses with marked temporal increases, in a bimodal pattern, …
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Rizobactérias e ácido jasmônico no controle de doenças do tomateiro
… e As e na atividade de enzimas de defesa: β-1,3-glucanases (GLU), quitinases (QUI), peroxidases (POX), polifenoloxidases (PFO), lipoxigenases (LOX) e fenilalanina amônia-liases (FAL), além da concentração de aldeído malônico (MDA). Os isolados foram aplicados nas plantas por microbiolização de …
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Tissue culture and transformation of introducing genes useful for pest management in rice
Sheath blight (SB), caused by Rhizoctonia solani K¨¹hn, is a major rice disease internationally and in the southern rice area of the Unites States, including Louisiana. Breeders have incorporated partial resistance into commercial rice varieties to control the disease, but a higher level of …