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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"germ line"”.
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Mechanisms governing transposable element expression in the Drosophila germ line
… active in the developmental context of the germ line, to fulfil their selfish drive to increase in copy number in the genome that will be passed to the next generation. Due to this threat, dedicated mechanisms have evolved to control the expression of transposons in the germ line, notably …
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Expression levels of non-autonomous retrotransposons in germ-line rodent tissues
… amplification. We propose that the level of germ-line transcription of SINE “master genes” is a primary factor in their successful mobility and vertical transmission. RT-PCR and qPCR results suggested higher expression of both SINE and LINE elements in germ-line tissues over somatic. …
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Joint Functions of METT-10 and Dynein in the Caenorhabditis elegans Germ Line
… gonad, GLP-1/Notch signaling instructs germ line stem cells to self renew through mitotic cell division. As germ cells progressively move out of the niche, they differentiate by entering meiosis and eventually form gametes. Using this model system, I uncovered a cooperative role for the …
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Computational imaging of dynamic nuclear processes in living somatic and germ line cells
Lichtmikroskopische Techniken können benutzt werden, um den Einfluss der räumlichen und zeitlichen Organisation von Chromatin und assoziierten nukleären Faktoren zu untersuchen. In der vorliegenden Arbeit möchte ich mit zwei biologischen Ansätzen die Bedeutung der quantitativen Extraktion von raum- …
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Characterization of Germ-Line Events in an X-Chromosome Duplication in Drosophila Melanogaster
Made available in DSpace on 2014-12-12T00:51:24Z (GMT). No. of bitstreams: 1 7309973.pdf: 2983929 bytes, checksum: a8d8c6bf729fb93c6e8ea7be31bcdbfa (MD5) Previous issue date: 1972
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Genetic Interactions of Factors That Regulate Alternative Rna Splicing In The Male Germ Line of Drosophila
… the regulation of alternative splicing affects germ cell development is also not well known. In this study, Drosophila Transformer 2 (Tra2) was used as a model to explore both the mechanism of its repressive function on its own pre-mRNA splicing, and the effect of the splicing regulation on …
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Molecular mechanisms governing germ line development in zebrafish and the role of this lineage in sexual differentiation
Während der Embryonalentwicklung des Zebrafisch (Danio rerio) werden die Urkeimzellen sehr früh von den anderen Zelllinien durch eine maternale, spezifische Form des Cytoplasmas, dem so genannten Keimplasma, unterschieden. Bei den nachfolgenden Zellteilungen verteilt sich dieses Keimplasma …
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A Genetic and Cytological Analysis of the Aberrant Behavior of an X-Chromosome Duplication in the Germ-Line of Drosophila Melanogaster
Made available in DSpace on 2014-12-12T00:51:25Z (GMT). No. of bitstreams: 1 7412122.pdf: 5572086 bytes, checksum: 7e4ca20b493fd546949a7b630078c610 (MD5) Previous issue date: 1973
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A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus
… as a master gene for the amplification of LINE, ID, and <em>mys</em> elements, which mobilize via RNA, in <em>Peromyscus maniculatus</em> tissues, by the use of the RT-PCR technique. It was concluded that potentially more than one master gene is responsible for the amplification of LINE and …
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Novel proapoptotic p63 isoforms are driven by an endogenous retrovirus in the male germ line of humans and great apes, likely increasing genome stability
TAp63, ein Homolog des Tumorsuppressors p53, hat die Fähigkeit, die weibliche Keimbahn zu beschützen, indem es Oozyten mit schweren DNA-Schäden nach Bestrahlung eliminiert. Obwohl die Expression von TAp63 in männlichen Keimbahnzellen vermutet wird, ist noch unklar, ob TAp63 auch als Wächter der …
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Discovering GCNA: A Novel Regulator of Germline Genomic Stability
Germ cells transfer genetic information across generations. Any change in germ line DNA is inherited by succeeding generations. Therefore, germ cell DNA must be protected from both internal and external assault. An advantage of sexual reproduction stems from the ability to generate variation by …
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The balance of poly(U) polymerase activity ensures germline identity, survival, and development in Caenorhabditis elegans
… elegans proteins, with in vitro PUP activity, in germline development. PUP-1/CDE-1 and PUP-2 are known to target certain classes of small non-coding RNA, and the function of PUP-3 remains unknown. We examined the developmental phenotypes of pup mutants and evaluated the expression of PUP proteins …
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Towards a Molecular Genetic Analysis of Spermatogenesis in Drosophila
… We generated a collection of enhancer trap lines that identify all cell types and stages of the developmental program. We used marker lines to show that somatic cell fates are altered in the absence of germ cells, suggestive of signaling events between germ line and soma during wild-type …
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High resolution alignment of the physical and genetic maps of the dpy-14 region of chromosome I in Caenorhabditis elegans
… or near the0.05 map unit unc-87-sP1 interval. Germ line transformation with cosmid clones containing C. elegans genomic DNA was used to generate stable transformants that carried heritable extrachromosomal arrays composed of exogenous DNA. The usefulness of these arrays as genetic mapping tools …
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The identification of novel factors required for the development of primordial germ cells
… detrimental to that single individual. However, germ line mutations can be passed on to the next generation. These mutations not only drive genome evolution but can also impair the fitness of the offspring. It has been shown that primordial germ cells (PGCs), the germ cells specified during …
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Role of the Rcd4:Ana3 sub-complex in Drosophila centriole duplication
… components for centriole development in the male germ line. Ana3 is essential for centriole formation during spermatogenesis but Rcd4 is not. My preliminary results indicate that Rcd4 may potentially contribute to the stabilisation of the cartwheel of spermatocyte centrioles. Nevertheless, further …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first time efficient enhancer trapping in fish. First, a transposon-based approach using the artificially …
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Role of BEC-1/Beclin 1 and Autophagy Genes in <i>C.elegans</i> Germline Cell Proliferation
… proliferation, using the <em>C. elegans</em> germ line as an <em>in vivo</em> model. BEC-1 is the <em>C. elegans</em> ortholog of human BECN1/Beclin 1, an essential autophagy regulator and tumor suppressor protein. We show that basal levels of autophagy are required for germline proliferation …
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CRISPR/CASTE: Functional Genomic Studies of the Major Evolutionary Innovations of Ants
… focus on generating and characterizing germ-line mutants. I believe that the study of mutants, and applying molecular biology methods more generally, can lead to insights in ant biology that would not be possible with more traditional methods. I first describe my efforts to develop a …
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Characterization of the human endogenous retrovirus HERV-‐K(HML-‐6) and HERV-‐K(HML-‐10) sequences and analysis of their expression
… from exogenous retroviruses that infected the germ line cells over millions of years and are termed human endogenous retroviruses (HERVs). After germ line infection, the retroviral DNA was integrated into the genome and transmitted through vertical transmission in the progeny by Mendelian laws. …
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