Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"germ layers"”.
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Effects of low oxygen culture on pluripotent stem cell differentiation and teratoma formation
… dishes and assessed differentiation to the three germ layers, endoderm, ectoderm, and mesoderm and to cardiomyocytes and assessed residual PSC within differentiated populations. Low P02 drastically affects differentiation of PSC to the three germ layers and cardiomyocytes. Overall, differentiation …
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Reprogramming cellular fate using defined factors
… to differentiate into tissues from all three germ layers. One of the ultimate goals of regenerative medicine is to isolate pluripotent stem cells from patients. Nuclear reprogramming offers the possibility of creating patient-specific cell lines, thus abrogating the need for immunosuppressants …
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CAMK-II: AN INTEGRAL PROTEIN IN CELL MIGRATION
… cell rearrangements to generate the three germ layers and shape the embryonic body. Three modes of cell migration must occur during vertebrate gastrulation and include: epiboly, internalization of the presumptive mesendoderm and convergent extension (C&E). C&E movements narrow the germ …
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Molecular Basis of Vertebrate Embryonic Migration
… than a ball of cells where the three primordial layers, the ectoderm, mesoderm and the endoderm are one on top of the other. The three germ layers will go on to form all the tissues and organs of the embryo. For example, the ectoderm will give rise to epidermis and the nervous system; the …
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Novel Mechanisms of Transcriptional Repression by the Paired-like Homeodomain Transcription Factor Goosecoid
Gastrulation is the process by which the three germ layers are generated during vertebrate development. Nodal ligands, which form a subgroup of the Transforming Growth Factor β (TGFβ) superfamily, regulate the expression several transcription factors implicated in gastrulation. Among these are the …
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Origins and Development of the Embryonic Vascular System in Xenopus
… and overexpression of Etv2 in any of the three germ layers causes activation of every endothelial marker examined. Along the way we have characterized a number of small-molecule inhibitors that should be useful to the Xenopus community and applicable to other model systems.
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Characterization of Genes Specifically Expressed in Embryonic and Cancer Stem Cells
… that differentiate into cells of all three germ layers. The stem cell genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc (c-Myc) act alone and interact with each other for mainintaining the stem cell state. These genes have been already studied well. The emergence of new …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… gastrulation, a pivotal event whereby the three germ layers: mesoderm, endoderm, and ectoderm are specified. Bone morphogenetic protein 4 (BMP4), secreted from the extraembryonic ectoderm is required for gastrulation to start whereby it activates downstream signaling molecules wingless inhibitor …
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Revealing a Non-canonical Role of Anti-apoptotic MCL-1 in Early Embryonic Development
… in differentiation into the three embryonic germ layers- ectoderm, mesoderm, and ectoderm. These data suggest that in addition to MCL-1’s required antagonism of cell death by the C-terminal region, MCL-1’s N-terminus is required for efficient cellular differentiation, potentially by …
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Characterization of cofilin and its potential role in the EphA4 pathway
… cells are relocating to create the three primary germ layers of the organism. Failure of this process to occur results in attrition of the embryos, frequently by way of embryonic exogastrulation. In order to characterize the role of the cofilin gene in embryonic development, we generated mutations …
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Integrin signalling in pluripotent cells acts as a gatekeeper of mouse germline entry
Primordial germ cells (PGCs) are the undifferentiated precursors of gametes and the sole mechanism by which genetic information is transmitted across generations through the germline. They appear at the proximal posterior region of the mouse embryo, where the somatic germ layers begin to emerge and …
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Providing a Malleable, Genetically Defined Cancer Model: A Porcine Solution
… cells derived from each of the three embryonic germ layers, isolated porcine ectodermic fibroblasts and endodermic keratinocytes were transformed using this method. This approach was then adapted to induce the transformation of T-cells, derived from the mesoderm, in vivo. The ability to …
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Optogenetic control of developmental signaling pathways
… pathways to pattern the embryonic tissue into germ layers from which the various tissues and organs emerge. A hallmark of embryonic development is that these recurring developmental signaling pathways are carefully orchestrated in space and time to facilitate proper development. Understanding …
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Towards using organoids in functional genomics – A single cell characterisation of mouse gastruloids
… and commit to one of the three main germ layers. While many of the key signalling pathways are well-known, the epigenetic processes underlying this lineage commitment remain poorly understood in mammals. Large-scale perturbation studies are necessary to functionally study the …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… of genes specifying non-neuroectodermal germ layers. This work demonstrates that NuRD can act in a complex and context-dependent manner to regulate TF binding dynamics, chromatin accessibility, genome organisation and ultimately transcription to coordinate cell fate decisions.
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Regulation of Zebrafish Gastrulation Movements by slb/wnt11
… lead to the formation of the three germ layers, ectoderm, mesoderm and endoderm. Recent studies have identified silberblick (slb/wnt11) as a key molecule that regulates gastrulation movement through a conserved pathway, which shares significant similarity with a signalling pathway …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… layer or ball of cells into the three primordial germ layers: the mesoderm, endoderm and ectoderm. In the mouse embryo, gastrulation starts with a group of asymmetrically positioned cells expressing a group of genes that include T/Brachyury. These cells undergo a directed epithelial to mesenchymal …
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Identification and characterisation of a novel, multi-potent, skeletal muscle-derived stem cell with broad developmental plasticity
… and in vivo giving rise to cell types from the 3 germ layers. PW1+ cells were identified and quantified with respect to location in the heart on 3, 10 day, 21 day and 2 year old mice with the majority of cells found within the epicardium. There was rapid decline in abundance during postnatal …
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Regulation of amnion induction during the human formative pluripotency transition
… to all somatic lineages and primordial germ cells. hPSC exists in distinct phases—naïve, formative, and primed—each with differing developmental potential. Naïve hPSC resembles the pre-implantation epiblast, retain trophectoderm (TE) potential, but are resistant to somatic lineages …
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Identification of proteins controlling gastrulation movements by a proteomic approach in zebrafish
… movements leads to the formation of the three germ layers: ectoderm, mesoderm and endoderm. In zebrafish, a model organism for vertebrate development, the mesendodermal progenitor cells separate from the ectodermal cells and migrate towards the animal pole. To identify proteins controlling …
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