Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"germ free"”.
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Genetic Manipulation of Bacteroides for Transient Colonization of Germ-free Mice
… tools that limited bacterial exposure of germ-free mice to a certain time-period was explored in this thesis. I target genes in synthesis pathways of two uniquely-bacterial amino acids, meso-diaminopimelic acid (mDAP) and D-Alanine (D-Ala) in a species of interest, Bacteroides …
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The effects of the three soil-inhabiting fungi on peanuts grown under germ-free conditions
Excised peanut embryos were sterilized by treatment with 1% NaOCl tor 5 minutes, and placed in 250-ml flasks containing nutrients and expanded granular shale. Nutrients consisted of Hoagland's solution supplemented with 1% sucrose and 0.03 ppm gibberellic acid. Plants were grown for 4 weeks in …
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To Investigate The Impact Of Gut Bacteria On Efflux Transporter Expression And Function In Gastrointestinal Mucosae
… (BCRP) using intestinal organoids derived from germ free and humanized mice. Typically, transport across the epithelial cell layers occurs by active transport and passive diffusion. Using mouse models that differ in bacterial content and fluorescence scanning, and live imaging techniques we …
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Protective role of lignan-converting bacteria on chemically-induced breast cancer in gnotobiotic rats
… cancer in gnotobiotic rats was investigated. Germ-free rats were associated with a defined lignan-converting consortium (Clostridium saccharogumia, Blautia producta, Eggerthella lenta, and Lactonifactor longoviformis). The rats colonized with lignan-converting bacteria consortium (LCC) were …
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Short Chain Fatty Acids Signaling through the G Protein-Coupled Receptor GPR41 in the Intestine.
… knock out mice were generated and rederived as germ free mice. Gpr41 knock out and wild type germ free mice were recolonized as young adults with 2 prominent members of the human distal gut microbial community: the saccharolytic bacterium, Bacteroides thetaiotaomicron and the methanogenic …
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Role And Impact Of The Gut Microbiota In A Drosophila Model For Parkinsonism
… to mammals, and efficient methods to rear germ-free flies. To take advantage of this potential, I examined the gut-brain axis in Drosophila models of autosomal recessive parkinsonism and observed a five-fold increase in the gut microbial load of aged parkin animals. The microbial load of …
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The Effect of the Microbiome on DNA Methylation
… and global DNA hypomethylation. Microbiota-free mice are known to live longer than their normal counterparts, and microbial dysbiosis is known to be a hallmark of colorectal cancer. In order to determine the microbiota’s ability to impact DNA methylation patterns, that in turn may influence …
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Unravelling the role of tryptophan metabolism in the microbiota-gut-brain axis: focus on the effects of stress and exercise
… we used well-recognised pre-clinical models (germ-free and antibiotic-treated mice) to decipher the role of gut microbes in the regulation of tryptophan metabolism and availability at baseline and following hypothalamic-pituitary-adrenal axis activation. We found that a 15-min acute stressor …
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The role of the gut microbiota in the function and integrity of brain barriers
… axis communication. In particular, using germ-free mice which lack a gut microbiota, we investigated the contributing role of the gut microbiota in regulation of the blood-CSF barrier. Following confocal microscopy for visualization of the tight junction protein, ZO-1, germ-free mice were …
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Functional Genomic Examinations Of Interactions Between Common Members Of The Human Gut Microbiota
… as well as by gene duplication. By colonizing germ-free mice with <italic>B. thetaiotaomicron,</italic> or <italic>B. vulgatus,</italic> or both species together, I documented that <italic>B. vulgatus</italic> upregulates its unique glycan-degrading enzymes to adapt to the presence of …
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Impact of the gut microbiota on DNA methylation in colorectal cancer
… and CpG island methylation by colonizing germ-free mice ApcMinΔ850/+;Il10–/– with E. coli NC101 & K. pneumoniae, specific pathogen free bacteria, and the mouse bacterial Consortium. Differential methylation analyses of adjacent normal colon tissue revealed a pronounced tissue side-specific …
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Interactions Between Microbial, Neuronal, and Immune Cells in the Digestive System
… the intestinal segment they occupy. By utilizing germ-free mice, we uncovered a stronger influence of the microbiota on distal intestine neurons, correlating with the region's higher bacterial density. Chronic antibiotic-mediated microbial depletion reinstated our findings in germ-free mice, …
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Aromatic Amino Acid Metabolome In The Human Gut Microbiota
… that regulates appetite. Monocolonization of germ-free mice with <em>Streptococcus</em> restored the physiological concentration of LacPhe in the ileum, and analysis of human microbiome datasets revealed the microbial LacPhe biosynthetic gene,<em> pep</em>V, was abundant in the GI tract and …
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Activation of proestrogens from hops by human intestinal bacteria: conversion of isoxanthohumol into the potent phytoestrogen 8-prenylnaringenin
… and low 8-PN producers. An experiment with germ-free and human microbiota associated rats and a human intervention trial confirmed the importance of variable intestinal IX activation and provided a definitive explanation for the variable biological activity of hop products. As the final …
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CNS Remyelination and the Gut Microbiota
… Secondly, I looked at remyelination in germ-free (GF) mice following cuprizone-induced demyelina- tion. As with the antibiotics-treated mice, there were deficits in inflammation following demyelination, which tended to peak later than in control mice. Finally, I investigated the …
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Metagenomic Analyses of the Human Gut Virome
… gut community structure, I colonized adult germ-free mice with a defined model human gut microbiota composed of 15 sequenced human gut symbionts, seven of which harbored 10 prophages, one of which: Bacteroides cellulosilyticusWH2) was represented by a library of >25,000 isogenic transposon …
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The Influence of the Microbiota on T Cell Development
… which had increasingly complex microbiotas; germ free (GF, with no microbiota), Oligo-Mouse-Microbiota (OMM12, with a gut microbiota which consists of 12 well defined bacterial species), and Specific pathogen free (SPF, with complex undefined microbiotas). To study the effects of the maternal …
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Microbial Metabolite Regulation of Microglial AHR Signaling in Alzheimer’s Disease
… with the lowest levels observed in pups and germ-free mice, and highest in aged wild-type mice, underscoring the importance of physiological context when analyzing AHR signaling. </p> <p>Conclusion: My findings demonstrate that AHR expression in microglia is altered by age, disease state, and …
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Novel insights into early life stress-induced dysfunction of the gut-brain axis
… modulators of visceral sensitivity using female germ-free mice. Here, we observed that the oestrous cycle modulated the visceral pain response in a microbiota-dependent manner and ovariectomy resulted in visceral hypersensitivity in conventional animals only. We then assessed alterations in the …
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Sympathy for the Microbiota: How Changes in Gut Microbial Composition Influence the Immune System and Basic Physiology by Way of the Sympathetic Nervous System
… comparing eEAN sensory and effector nodes from germ free and specific pathogen free mice. RNA sequencing revealed changes in activity-dependent transcripts in afferent sensory neurons in the nodose ganglion and efferent sympathetic neurons in the CG-SMG. These results served as an entry point to …
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