Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"genotoxic agents"”.
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p53 nuclear localization control, and p53-dependent regulation of DNA repair gene transcripts
… fibroblasts were treated with a panel of genotoxic agents, and assayed for p53-dependent upregulation or downregulation of the approximately 15,000 gene sequences represented on the microarray. New candidate p53 target genes were revealed, among them the DNA repair gene ErccS, which …
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Mismatch Repair Protein-Dependent Cytotoxic Signaling In Cells Treated With The Cancer Chemotherapeutic Cisplatin
… participate in cytotoxicity induced by specific genotoxic agents, including cisplatin (CDDP, cis-diamminedichloroplatinum(II)), a cancer chemotherapeutic drug utilized clinically to treat a variety of malignancies. The MMR protein complex MutSα (consisting of the MSH2 and MSH6 proteins) binds to …
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In vivo and in vitro applications of biospectroscopy : potential use as a diagnostic tool which can provide insights into cell cycle dependent proliferation
… pre-determinants of MCF-7 cell susceptibility to genotoxic agents. Biospectroscopy and computational analysis produced biochemical signatures characterising dose and cell cycle related effects of B[a]P that indicated DNA alterations being induced. Overall the cell cycle is an important factor in …
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Visualising the Mycobacterial Mutasome
… ImuA′ and ImuB in live mycobacteria exposed to genotoxic agents. Using fluorescence microscopy, it was observed that, during prolonged genotoxic stress, single M. smegmatis cells exhibited an elongated cell phenotype and apparent aneuploidy – potentially providing an environment for …
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Developing Methods for Enhanced Measurement of DNA Single-Strand Breaks and Somatic Variants
… mutations can be accelerated through exposure to genotoxic agents that introduce lesions which, if left unrepaired, prevent accurate replication of the genome. As such, it is crucial to understand the ways in which DNA becomes damaged, how cells respond to various types of damage, and how this …
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ASSESSING THE GENOTOXICITY OF TRICLOSAN IN TADPOLES OF THE AMERICAN BULLFROG, LITHOBATES CATESBEIANUS.
… in urban areas. The NIH has pinpointed genotoxicity as a major route of cancer causation, and has since developed stringent testing procedures for potentially hazardous chemicals. One such method, recognized for its simplicity and economy, is the micronucleus assay. A study was conducted …
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Stimulation of chemotherapy-induced immunity by targeting IL-6 in the tumor microenvironment
… preclinical studies of a group of cytostatic and genotoxic agents has shown that these drugs exert their effects on cancer cells by, in part, boosting the functions of immune cells. However, the vast majority of these agents do not effectively engage the immune system when used as therapies for …
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DNA-damage-mediated remodeling of normal and tumor microenvironments modulates cell survival
… regimens involve the systemic administration of genotoxic compounds that induce cancer cell death via well-established DNA damage response signaling networks. While modern chemotherapeutic regimens can be curative, chemotherapeutic drug resistance remains a major clinical problem. This drug …
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Modulation of Clr-b Ligand for the Inhibitory NKR-P1B NK Cell Receptor in Response to Transformation and Genotoxic Stress
… receptors. The recognition of targets undergoing genotoxic stress or transformation by NK cells has been limited to the upregulation of stimulatory NKG2DL and “missing-self” recognition of MHC-I. The ligand pair NKR-P1B:Clr-b participates in “missing-self” recognition of tumour and infected cells, …
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Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells
… are unusually sensitive to DNA crosslinking agents and it is now known that FA cells lack one of twenty-two known different FA complementation group (FANC) proteins involved in the repair of DNA interstrand crosslinks (ICLs). More recently, two DNA glycosylases (endonuclease VIII – like 1 & 3 …
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Deciphering the role of BRCA2 at the damage-induced G2 checkpoint
… and the therapeutic response of tumours to genotoxic agents. The large 3418 residue protein encoded by BRCA2 – heterozygous germline mutations in which predispose to cancer - has recently been implicated in G2 checkpoint maintenance. However, the mechanistic basis of BRCA2’s role in the G2 …
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Novel functions of N-acetyltransferase 10 (NAT10) in DNA repair and replication with potential implications for premature ageing syndromes
… of DNA double-strand breaks (DSBs), highly genotoxic lesions that can result in unrestrained cellular lethality or genomic instability. During this project, I showed that NAT10 also plays a role in the repair of DSBs. Specifically, I observed that NAT10 localises to DSBs, dependent on the …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… most pervasive endogenous and radiation-induced genotoxic agents induce strand breaks and a plethora of base lesions in DNA that (except double-strand breaks) are repaired via the DNA base excision repair (BER) pathway. Four mammalian DNA glycosylases, namely, OGG1 and NTH1 in the Nth family, and …
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Role of NF-κB p65 subunit phosphorylation by ATM in DNA damage-regulated gene expression
DNA damage, induced by several genotoxic agents, challenge genome integrity. Among the different types of DNA lesions, DNA double-strand breaks (DSB) are the most deleterious. Indeed, if not correctly repaired, they can lead to cell death or carcinogenesis. Upon DSB, cells activate a complex …
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Identification and characterization of novel autoregulatory mechanism controlling ataxia telangiectasis mutated gene expression, protein traffiking and function
… cells which are constantly challenged by genotoxic agents from a variety of sources and therefore require a robust sensing and repair mechanism to maintain cell vitality. Cells lacking ATM are hypersensitive to cytotoxic insults, particularly genotoxic stress, induced through radiation or …
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Leveraging cell micropatterning technology for rapid cell-based assessment of chemical toxicity and population variation in toxicity susceptibility
… safety testing is the assessment of a compound's genotoxic potential. In this work, we developed a CometChip platform that addresses two major areas that are lacking in genotoxicity testing: 1. rapid and sensitive detection of bulky DNA adducts, and 2. robust and physiologically relevant …
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Regulation of FBP1 expression and changes in glucose metabolism are crucial for an appropriate response to DNA damage and aging in S.cerevisiae
… of living organisms to smaller amounts of toxic agents and other adverse effects may be more common in natural environments than direct impact of highly cytotoxic doses of the same agents. Occurring over various time spans or as the consequence of repeated exposures, the accumulation of mutations …