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Showing 1 to 18 of 18 for “"genomic clone"”.
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Isolation and characterization of a synthetic gene and a genomic clone encoding acyl carrier protein from Escherichia coli
… Normally apo-ACP is not detected in vivo. A genomic clone encoding ACP has also been isolated and sequenced. The ACP gene (called acpP) was located on the genetic map between fabF and fabD which encode two fatty acid biosynthetic enzymes, 3-ketoacyl-ACP synthase II and malonyl CoA-ACP …
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Isolation and sequencing of a genomic clone of ESI28 : an early salt-stress induced gene of the salt-tolerant wild wheatgrass Lophopyrum elongatum
A genomic sequence corresponding to a putative actin-depolymerizing factor (ADF) was isolated from a wheat (Lophopyrum elongatum) genomic library. The genomic clone, designated ESI28-G-5, codes for the ESI28 gene (early salt-stress induced). The 5$\sp\prime$-upstream sequence contains a putative …
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Cloning and Characterization of the Salt Overly Sensitive 1 (SOS1) Gene in Chenopodium quinoa WILLD.
… cDNA sequence of 3490 bp and a full length genomic clone of 21314 bp. Sequence analysis predicts the quinoa SOS1 homolog spans 23 exons and is comprised of 3474 bp of coding sequence (excluding the stop codon). Introns comprise 17840 bp of the genomic clone and range in size from 77 to 2123 …
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NAT2/AAP1 as a Prototypical Example of the AAP Amino Acid Transporter Gene Family: From Protein Topology to Regulation of Gene Expression
A genomic clone of NAT2 was isolated and sequenced. The 5' -flanking region contains several consensus recognition sites for putative transcription factors. I have now shown that NAT2 expression is nitrate and light inducible, but not circadian-regulated. Since the nitrate assimilatory enzymes are …
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Characterization and Transcriptional Control of the Rabbit Cytochrome P450pbii Genes (Evolution, Isozymes)
… mapping and DNA sequencing lambda phage genomic clones corresponding to the genes for P-450PBc2, P-450PBc3 and P-450 (form 1). Comparison of the cloned genomic sequences with cDNA sequences established that genomic clone 6 contained the 5'-flanking region and the first six exons of the …
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Polymorphism of bovine MHC class II genes: Association with persistent lymphocytosis caused by bovine leukemia virus
… 13 of which were PL. A 783 bp BoLA-DQA cDNA clone $(\alpha$5) was identified by screening a bovine cDNA library. The $\alpha$5 clone has an average nucleotide similarity of 91.6%, 86.0% and 84.2% with a BoLA-DQA genomic clone, SLA-DQA, and HLA-DQA, respectively. In contrast, similarity was …
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
Putative cotton cDNA clones and cognate genomic clones for a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE) and an osmotin-like pathogenesis-related 5 (PR5) protein have been isolated and characterized. PATE is a class B fatty acid thioesterase with specificity for saturated long-chain …
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Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.
A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… of similarity with yeast CDC48p was isolated. A genomic clone was isolated using the partial length cDNA clone as a probe, and its sequence was used to design oligonucleotide primers for polymerase chain reaction experiments that facilitated cloning and reconstructing a full length, 3.4-kb cDNA …
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Molecular cloning and analysis of the genome of bovine parvovirus
The genome of bovine parvovirus (BPV) has been cloned by blunt end ligation of double-stranded virion DNA into the plasmid pUC8. The resulting genomic clones were infectious after transfection into bovine fetal lung (BFL) cells. Sequencing of the plasmids demonstrated that deletions were common at …
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Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
… complete cDNAs, Cmchi1 and Cmchi2, and a partial genomic clone of Cmchi2 have been isolated from muskmelon seeds. Cmchi2 gene has two introns in the coding region while Cmchi1 is intronless. Cmchi1 cDNA encodes a class III chitinase while Cmchi2 cDNA encodes a class II chitinase. Cmchi1 and Cmchi2 …
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Characterization of a murine activated lymphocyte antigen (MALA-2) : the murine homologue of human intercellular adhesion molecule 1 (ICAM-1)
… MALA-2 was characterized by cDNA cloning, genomic cloning, and analysis of an assumed alternatively spliced mRNA. Two cDNA clones were isolated from an NS-1 cDNA library using oligonucleotide probes constructed from amino acid sequences of peptides derived by tryptic cleavage. The two …
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Characterization of the cytochrome P-450IIC3 gene and 5' flanking region of P-450IIC1
… induced by phenobarbital. Two rabbit liver DNA genomic libraries were screened for the presence of the cytochrome P-450IIC3 and IIC1 genes. DNA from genomic clones C3.1, C3.2, C3.3, and C1.1 were isolated, and subcloned into phage M13 mp11 and phagemid pTZ18R for sequencing by the chain …
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The role of steroidogenic factor-1 (SF-1) in gonadotropin-releasing hormone (GnRH) receptor gene regulation
… regulation of GnRHR gene expression. A genomic clone of the mouse GnRHR gene contains a putative SF- I site at about -15 relative to the translation start site. We demonstrate the presence of a factor with SF-1-like DNA-binding activity in the gonadotrope cell lines, αT3-1 and αT4, by …
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Transcription and encapsidation in parvoviruses LuIII and bovine parvovirus
… plus and minus DNA strands equally, were cloned and sequenced. The left and right termini of LuIII differ in nucleotide sequence and these termini can assume T- and U-shaped intra-strand base-paired structures, respectively. The LuIII termini are virtually identical in nucleotide sequence …
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Adding to the recombineering toolbox: interrogating internal transcriptional complexity in Caenorhabditis elegans operons via recombineered fosmid-based reporters
… style reporters built directly from genomic clones in which an entire operon structure is contained within the insert sequence. Tagging each operon gene with a different FP would permit operon gene expression analysis within the genomic context of that operon.<br/>To facilitate …
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The molecular biology of calbindin-D₉
… human and porcine calbindin-D9k at the cDNA and genomic level. The cDNAs for the bovine, murine, and ratcalbindin-D9k as well as the gene for rat calbindin-D9k had been cloned previously. The full length cDNA encoding the human and porcine calbindin-D9k were cloned using a modified PCR …
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Cellular factors and viral elements for parvovirus replication
… nucleotides in the BPV life cycle, two mutants clones lacking the bubble region were constructed and their replicative properties were analyzed after electroporation in permissive cells. Infectivity of the mutant clones was determined by three techniques: observation of cytopathic effect, …