Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"genome-wide screen"”.
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A Genome-Wide Screen on Modifiers of Tau-Induced Neurodegeneration Using RNAi-Mediated Gene Silencing in Drosophila
… beitragen, wurde ein genomweiter Screen in einem etablierten Drosophila-Modell durchgeführt: eine transgene Fliegelinie, die eine FTDP-17-assoziierte Variante (Tau[R406W]) des Microtubuli-assoziierten Proteins Tau exprimiert. Die Expression von Tau[R406W] im Facettenauge von …
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Genes with internal repeats require the THO complex for transcription
A genome-wide screen for promoter-independent regulators of the Saccharomyces cerevisiae adhesin FLOII identified 37 novel regulators. Among the mutants with the strongest phenotype were the deletions of genes encoding subunits of the evolutionarily conserved THO complex. The THO complex, which is …
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Investigation into the genetic nature of familial congenital bicuspid aortic valve
… at the D3S1259 marker locus in a previous 20 cM genome-wide screen. Linkage analysis with additional markers in the region suggested that the initial hit at D3S1259 had probably been obtained by chance in most families. We are currently recruiting additional families and plan to undertake a 10 cM …
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Characterization of Drosophila Scap: Analysis of Mutants and Evidence for a Retention Factor
… of the dSREBP, I set up a high through-put genome-wide screen. Employing luciferase as reporter, knocking-down each gene in genome through RNA interference will reveal the genes that modulate the activity of dSREBP.
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Global and chromosome-specific consequences of aneuploidy in Saccharomyces cerevisiae
… previously lacking. We performed high-throughput screens for genes whose deletion has a synthetic fitness cost in aneuploid Saccharomyces cerevisiae cells containing single extra chromosomes. This analysis identified genes that when deleted decrease the fitness of specific disomic strains as well …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
… anti-leukemia stem cell activity, we performed a screen and identified the antimicrobial tigecycline (TIG). Methods: Primary AML mononuclear cells were isolated by Ficoll centrifugation from peripheral blood. Flow cytometry dye; JC-1, Carboxy-H2DCFDA, Mitotracker GreenFM. Leukemia stem cell …
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The Protumorigenic Role of Caspase-8 in Neuroblastoma
… that has been reported to be phosphorylated in a genome-wide screen for ATM substrates. Here, we tested whether casp8 expression enhanced primary NB tumorigenesis and whether modulation of casp8 by phosphorylation altered its biological function in NB. For the first time, this study demonstrates …
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Finding the Best Gene Candidate(s) as a Potential Therapeutic Target for N-MYC Overexpressing Neuroendocrine Prostate Cancer (NEPC)
… cancer cells. The Vizeacoumar lab performed genome-wide shRNA and CRISPR screening to identify genes that become essential in N-MYC overexpressing cells. My work will specifically focus on validating three candidate genes (WNT1, SOX17, and FOXN1) identified from the genome-wide screen. …
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Studies on the mechanisms that contribute to the endoplasmic reticulum quality control system in Saccharomyces cerevisiae
… degradation and forward traffic. Second, a genome-wide screen was conducted to identify novel players involved in ER protein retention and export. For this purpose, extracellular secretion of the ER resident protein, Kar2p, was monitored in strains of the yeast gene deletion collection. We …
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Lipid rafts in protein sorting and yeast cell polarity
… cerevisiae. To this end, we conducted a genome wide screen that identified yeast mutants defective in cell surface delivery of the model cargo protein FusMid-GFP. The most striking result of this screen was that mutant strains with defects in ergosterol (the major yeast sterol) and …
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Identification and characterisation of murine metastable epialleles conferred by endogenous retroviruses
… represent approximately half of the mammalian genome. Epigenetic mechanisms evolved to repress these potentially deleterious mobile elements. However, such elements can be variably silenced between individuals – so called ‘metastable epialleles’. The best known example is the Avy locus where an …
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Analyzing Genomic Studies and a Screen for Genes that Suppress Information Loss During DNA Damage Repair
… minimizes genetic degradation, I performed a screen for genes affecting this process. >In devising this study, I set out to quickly quantify the contribution of every non-essential yeast gene to suppressing genetic rearrangements and deletions at a single locus. Before I began I did not fully …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
… in unlocking the full potential of naïve PSCs. A genome- wide screen identified novel regulators of naïve cell reprogramming, unexpectedly highlighting the Polycomb Repressive Complex 1 (PRC1) subtype PRC1.3. In this project, I sought to understand the role of PRC1.3 in human pluripotency and …
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Translational tuning pre-emptively modulates protein folding and secretory pathway defects
… extrude the mitochondrial toxin, oligomycin. A genome wide screen was previously performed in our lab to identify factors that specifically contribute to biogenesis of a misfolded version of this protein, Yor1-F. The main focus of my thesis work is to investigate translation regulators required …
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Bioinformatics approaches to analysing RNA mediated regulation of gene expression
The genome can be considered the blueprint for an organism. Composed of DNA, it harbours all organism-specific instructions for the synthesis of all structural components and their associated functions. The role of carriers of actual molecular structure and functions was believed to be exclusively …
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Molecular basis of life-history evolution: a tale of two insects
… traits. Given that increases in lifespan have widespread influence on reproductive output, molecular geneticists interested in aging have documented survival costs of reproduction in long-lived individuals. Mutations in signaling pathways that couple environmental signals to key physiological …
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Genome scale identification of HIV Dependency factors across multiple HIV-1 strains
… thesis, I developed a method called HIV-CRISPR screening at genome-wide scale, and then at a smaller, targeted scale, to uncover novel genes that may act as HIV-1 dependency factors. By using a screening technique which assesses enrichment or depletion of guide based on HIV-1 release from …
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Epigenetic repression of intronless mobile elements by the HUSH complex
The mammalian genome is under constant threat from invasion by mobile genetic elements including transposons and viruses. To defend the genome, cells recognize incoming DNA and limit its transcription through repressive chromatin modifications. The human silencing hub (HUSH) complex …