Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"genome-wide CRISPR/Cas9 screen"”.
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Identification of Novel Regulatory Genes in Acetaminophen Induced Hepatocyte Toxicity by a Genome-Wide CRISPR/Cas9 Screen
… toxicity or liver injury. This study used a genome-wide CRISPR/Cas9 screen to evaluate genes that are protective against or cause susceptibility to APAP-induced liver injury. HuH7 human hepatocellular carcinoma cells containing CRISPR/Cas9 gene knockouts were treated with 15mM APAP for 30 …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
… its pathogenesis, I developed and carried out a genome-wide CRISPR/Cas9 knockout screen using a tissue culture model of epithelial cell infection. I used a hierarchical validation method which combined arrayed high-content microscopy and semi-automated image analysis to confirm a novel set of …
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Interrogating DNA replication stress and DNA damage responses via phenotypic and functional CRISPR/Cas9 screens
… DNA lesions, posing a constant threat for genome integrity and cell survival. Consequently, various DNA repair processes and pathways have evolved to deal with different DNA lesions and ensure genome stability. The tumour suppressor ATM is one of the apical kinases in the DNA damage …
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Epitranscriptional Control of the Caspase-2—MDM2—p53 Axis: The Role of the m6A Writer in p53 Signaling.
… response to centrosome-derived stress. Using a genome-wide CRISPR/Cas9 screen coupled with a fluorescent reporter system, we uncovered all seven components of the m6A writer complex (METTL3, METTL14, WTAP, VIRMA, RBM15, ZC3H13, and CBLL1) as top-ranking hits required for proper activation of the …
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Defining Determinants of Primary Drug Resistance in Precision Cancer Therapies
… unbiased pharmacological and functional genetic screening approaches to overcome the persistent problem of primary drug resistance in two cancer contexts: (1) epidermal growth factor receptor (EGFR)-driven triple-negative breast cancer (TNBC) and (2) PIK3CA mutant gastric cancer. Particularly, in …
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The role of IFN-γ in cell-autonomous immune responses against Salmonella Typhimurium and Toxoplasma gondii
… The research described in Chapter 2 used a genome-wide CRISPR-Cas9 screen as an unbiased approach to identify new IFN-γ-induced genes that play a role in the cell death pathway. This viability screen enriched for cells resistant to LPS-mediated cell death after IFN-γ priming. Their …
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DISSECTION OF HOST-VIRUS INTERACTIONS IN THE HUMAN CENTRAL NERVOUS SYSTEM
… infection to either IAV or MPXV. By developing a genome-wide CRISPR/Cas9 screening platform in human neurons, we were able to identify several candidate host factors that regulate neuronal anti-HSV-1 immunity, such as MTBP, SIRT7, GNAI3, NPPA, POLR3E and RNGTT. In a complementary approach, we …