Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"genome-scale model"”.
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Metabolic network reconstruction and genome-scale model of butanol-producing strain clostridium beijerinckii ncimb 8052
… from a systems viewpoint using constraint-based modeling allows for simulation of the global effect of various genetic modifications. We present the first genome-scale metabolic model (iCM925) for C. beijerinckii, containing 925 genes, 938 reactions, and 881 metabolites. To build the model we …
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Total proton flux and balancing in genome-scale models: The case for the updated model of Clostridium acetobutylicum ATCC 824
Genome-scale modeling and new strategies for constraining these models were applied in this research to find new insights into cellular metabolism and identify potential metabolic engineering strategies. A newly updated genome-scale model for Clostridium acetobutylicum, iMM864, was constructed, …
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Systems metabolic engineering through application of genome-scale metabolic flux modeling
… in systems metabolic engineering involves genome-scale metabolic flux modeling. These models allow generation of predictions of the global metabolic flux distribution in the metabolic network of organisms, in silico. With the current advances in genome sequencing technologies and the global …
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Efficient enzyme coupling algorithms identify functional pathways in genome-scale metabolic models
… whose fluxes are “coupled"" or correlated in genome-scale models. By identified sets of coupled reactions, modelers can 1.) reduce the dimensionality of genome-scale models, 2.) identify reactions that must be modulated together during metabolic engineering, and 3.) identify sets of important …
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Systems metabolic engineering of Arabidopsis for increased cellulose production
… methods, predicting metabolic functions with genome-scale models had shown promising results in the recent years. Using sophisticated algorithms, such as flux balance analysis, OptKnock, and OptForce, we can predict flux distributions and design metabolic engineering strategies at a greater …
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Genome-scale metabolic modelling of Salmonella and Lactobacillus species
… mortality in humans. It is also a commonly used model organism for intracellular Gram negative pathogens, a group of bacteria that is becoming increasingly resistant to available antibiotics. Systemic Salmonella infection involves proliferation in the small intestine followed by infection of …
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Identification of novel properties of metabolic systems through null-space analysis
Metabolic models provide a mathematical description of the complex network of biochemical reactions that sustain life. Among these, genome-scale models capture the entire metabolism of an organism, by encompassing all known biochemical reactions encoded by its genome. They are invaluable tools for …
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A systems biology approach to understanding and improving butanol production in clostridium beijerinckii
… my Ph.D. dissertation, I used constraint-based modeling and metabolic profiling to holistically investigate C. beijerinckii metabolism, with the ultimate goal of garnering new insights to guide strain design for increased butanol production. The work described herein makes four important …
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Development of Steady-State and Dynamic Flux Models for Broad-Scope Microbial Metabolism Analysis
… a holistic review of optimal fluxomes on the genome scale. In this dissertation, flux analysis techniques were constructed to investigate the microbial metabolisms.</p> <p>First, an open-source and programming-free platform of 13C-MFA (WUFlux) with a user-friendly interface in MATLAB was …
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Model-guided Analysis of Plant Metabolism and Design of Metabolic Engineering Strategies
… This has enabled researchers to use models and data to design experiments and guide new discovery as well as test for consistency. One such computational method is constraint-based metabolic flux modeling. This is performed using genome-scale metabolic models (GEMs) that are a …