Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"genome structure"”.
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Transcriptional regulation and genome structure
… of the gene. Thus the structuring of the genome into DNA loops is important in the control of gene expression. This thesis will focus on the role of genome structure in transcriptional regulation. Two key questions in this area that I have attempted to address during my PhD are "how are …
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3D Chromatin Structure and Gene Regulation: Integrated Genome Structure, Transcriptomics and Epigenetics
The three-dimensional (3D) organization of the genome is essential for regulating gene expression, replication, and stability. Chromatin folding mediates enhancer–promoter communication, but bulk Hi-C data obscure single-cell variability, while sparse single-cell Hi-C limits structural resolution. …
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Insights into gene regulation by genome structure, phase separation and developmental signaling
… provide new insights into gene regulation by genome structuring, phase separation and developmental signaling.
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Cytogenetics and genome structure in genus Miscanthus, a potential source of bioenergy feedstocks
… This study (chaper 2) combines information on genome size and ploidy levels and DNA sequence variation to classify Miscanthus accessions to aid potential biomass crop improvement programs and to investigate the evolution of the genus. We observed that Miscanthus accessions fell into 4 groups, …
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Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer
… maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of …
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Exploring Genome Structure and Gene Regulation Related to Virulence in Fungal Phytopathogens Using Next Generation Sequencing Techniques
… Applications of NGS include studies of entire genomes, characterization of the entire transcriptome (RNA-Seq), and detection of protein-DNA binding sites (ChIP-Seq). The cost of sequencing a fungal genome, for example, has decreased from more than one hundred thousand dollars to currently only …
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MicroRNA-122 and Poly(C)-Binding Protein 2 Bind to the Hepatitis C Virus Genome, Regulating Viral Replication and Genome Structure
Hepatitis C virus (HCV) has a positive-sense RNA genome which is used as the template for both viral translation and negative-strand RNA synthesis. As both processes work in opposite directions on the genome the virus requires a mechanism to coordinate what process is acting on a given copy of …
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Scalable Methods for In Situ Genomics
The maxim that biological structure determines function was inspired by the discovery of the DNA double helix, yet mapping the structure of genomic DNA within a cell remains challenging, and accordingly the role of genome structure and organization in determining cellular function is an open …
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
<p>The three-dimensional organization of the genome is critical for regulating gene expression and maintaining cellular identity. The organizing principles underlying this 3D structure are a subject of much study, and it has proven to be a complex system to unravel. There are multiple scales of …
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The innate immune response to Mycobacterium tuberculosis is dependent on strain lineage and on host population
The genome structure of Mycobacterium tuberculosis is strongly clonal, in the absence of horizontal gene transfer. Thus it is feasible that clonal lineages may exhibit particular phenotypic characteristics, which may, in turn, result in differences in virulence or influence their association with …
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Mechanisms and impact of alternative transposition-induced segmental duplications
… are prevalent in both plant and animal genomes, and have played important roles in genome evolution. The focus of my project is to understand the transposition-mediated mechanisms that lead to the formation of segmental duplications, and the immediate impact of recently generated large …
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Partners in space: Discordant population structure between legume hosts and Rhizobium symbionts in their native range
… study how genetic variation in both partners is structured in space. This is because, in coevolution, the spatial distribution of genetic variation in one species can both determine its ability to locally adapt to a partner species and also serve as an agent of selection acting on that partner …
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Genome organization in transcriptional regulation
… of cell identity, development, and disease. Genome organization contributes to transcriptional regulation in multiple ways. At a fundamental level, the genome is organized into distinct active and repressive chromatin states that facilitate transcriptional regulation. These chromatin states …
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Analysis of the genome organization and fitness traits of non-pathogenic Escherichia coli strain Nissle 1917 (O6:K5:H1)
In the last years more than one hundred microbial genomes have been sequenced, many of them from pathogenic bacteria. The availability of this huge amount of sequence data enormously increases our knowledge on the genome structure and plasticity, as well as on the microbial diversity and evolution. …
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INSIGHTS INTO THE GENETIC DIVERSITY OF ASIMINA TRILOBA: A STUDY USING GENOME ASSEMBLY AND POPULATION GENETICS
… this, we have produced a high-quality reference genome by assembling a draft genome using PacBio's Sequel II long reads and polishing with Illumina short reads. We first used a genotype-by-sequencing (GBS) to genotype 124 individuals from 28 sites across the state of Virginia to produce a set of …
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The role of MLL4 in the chromatin framework: from the epigenome to 3D genome organization
… haploinsufficiency, TrxG members show reduced genome binding, while PcG strengthens its enrichment and genome occupancy. This chromatin state rearrangement is also reflected on a three-dimensional scale. Genome Architecture Mapping (GAM) experiments reveal distinct changes in 3D chromatin …
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Role of DNA replication timing in gene expression and chromatin organisation
… in cancers. While temporal changes in genome duplication are associated with altered transcription and chromatin organisation, it is still unknown whether DNA replication timing (RT) is a cause or a consequence of cellular fate changes. During my thesis I used a conditional system to …
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In situ genome sequencing: genomic measurement at the convergence of structure and molecular identity
… Microscopy directly provides rich information on structure and organization, while sequencing directly provides rich information on the identities of molecular species. Both microscopy and sequencing have developed the resolution needed for molecular characterization of cells: respectively, they …
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Antibody production with synthetic peptides against human Coronavirus-NL63 ORF3 protein
… are bats and palm civets. A 27,553‐bp CoV‐NL63 genome structure and the spike glycoprotein (S1 domain) use angiotensin‐converting enzyme 2 (ACE2) for infectious entry. Furthermore, CoV‐NL63 simplifies binding to ACE2 by attaching to heparan sulphate proteoglycans on the cell surface. ACE2 has a …
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