Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"genome organisation"”.
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Study of 3D Genome Organisation in Budding Yeast by Heterogeneous Polymer Simulations
… the nucleus has revealed the essential role of genome organisation in controlling genome function. Furthermore, genome architecture is highly dynamic and significant chromatin re-organisation occurs in response to environmental changes. However, the mechanisms that drive the 3D organisation of …
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Investigating 3D genome organisation in Caenorhabditis elegans with Accessible Region Conformation Capture (ARC-C)
… of Hi-C, only survey a pre-defined subset of the genome. I developed a variant of Hi-C, named Accessible Region Conformation Capture (ARC-C), in C. elegans, which interrogates genome organisation at multiple scales genome-wide - from domains and compartments to high resolution interactions between …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… (TF) binding dynamics, chromatin accessibility, genome organisation and ultimately gene expression in naive pluripotency through to lineage specification. Single-molecule tracking of pluripotency-associated TFs in live mouse ESCs revealed that remodelling by NuRD acts to actively promote the …
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The association between sperm aneuploidy and male infertility: Screening, aetiology and possible routes to alternative therapy
… specific chromosome pairs? What is the role of genome organisation in male gametes and its association with infertility? Whether use of alternative therapy (in this case, traditional Chinese Medicine (TCM)) can be used to improve sperm disomy levels. Statistical analysis of questionnaire data …
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Insights into transcriptional regulation from natural and induced variation in closely related species
… functional contexts, such as higher-order genome organisation or the neoplastic transformation of a tumour cell in cancer development. In the first part of the thesis, I explore the evolutionary dynamics of the CTCF (CCCTC-binding factor) binding among mouse species and its interplay with …
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The role of the SWI/SNF ATP dependent chromatin remodelling complex in the regulation of the human hair follicle cell proliferation and control of the human cutaneous wound healing
… as well as higher order chromatin folding and 3D genome organisation. ATP-dependent chromatin remodelling complexes modulate nucleosome structure, positioning and chromatin de-compaction and are involved in gene activation and repression. SWI/SNF ATP-dependent chromatin remodelling complexes …
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Spatial organisation of proto-oncogenes in human haematopoietic progenitor cells
… the context of this functional framework, the genome is organised, allowing contact between specific genomic regions and sub-compartments. Previous work has shown that genes in both cis and trans can make specific contacts with each other. I hypothesise that such a preferred juxtaposition may …
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Flagellum assembly in protozoan parasites
… before exflagellation. This detailed 3D organisation of flagellated microgametes provides new insights into the mechanisms of axoneme assembly and haploid genome organisation in malaria parasites.
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Genome-Wide and Multi-Scale Mapping of DNA Supercoiling in Differentiating Human Stem Cells
… programme of every cell is encoded in its genome. Human cells must organise and compact their genetic material at multiple levels to accommodate the two-meter-long DNA within the confined nuclear space of six microns. Chromatin folding is not only a cellular need, but is also crucial for …
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Single-molecule microscopy reveals details of heterochromatin reorganisation at the onset of mouse ES cell differentiation
In the last decade, spatial organisation of the genome has been recognised as one of the important factors linked to gene expression and cell identity. Although we have come a long way in describing how chromatin folds – from the level of single nucleosomes all the way to compartments and …
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Molecular genetic investigation of autosomal dominant muscular dystrophy
This thesis contributes to the Human Genome Project by adding detail to the physical and genetic maps of the human genome, and by identifying a strong candidate gene for a form of distal myopathy. Genomic clones for the human skeletal muscle genes slow troponin (TNN/1), alpha actin (ACTA1), and …
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… applied a novel network visualisation method for genome-wide promoter interaction data generated by promoter capture Hi-C. The availability of high quality human pluripotent stem cell datasets allowed me to utilise the new techniques to further our understanding of the dynamics of genome …
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… cell types, it remains unclear how a single genome can support hundreds of distinct fates. Combinatorial transcription factor binding explains part of this complexity, but it does not fully account for the diversity of regulatory programs, pointing to additional layers of encoding beyond …
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Engineering Escherichia coli as a general genome synthesis platform
Writing DNA at genome scale provides unprecedented control in engineering biological systems. This includes the ability to reprogram the genetic code to expand biological functionality and equips biologists with tools to ask previously unanswerable questions on genome organisation and regulation. …
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The evolution of gene regulatory landscapes in mammalian tissues
… studied. To fill this gap, I analyzed whole-genome bisulfite sequencing (WGBS) and matched chromatin immunoprecipitation assays followed by sequencing (ChIP-seq) of five TFs from the livers of five mammals. I first characterized DNA methylation patterns at transcription factor binding regions …
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The First Insights into the Phylogeny, Genomics, and Ecology of the Novel Bacterial Phylum Armatimonadetes
… by the sequencing of C. calidirosea T49T genome and analysing concatenated amino acid sequences of conserved genes against homologs in other prokaryotic genomes. The phylogenomic analysis showed Chloroflexi to be the most closely related formal phylum to Armatimonadetes. This publication …
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Investigation of the three dimensional organisation of the genome in B cell development
… at the pro-B cell stage, but undergoes reorganisation at the pre-B cell stage. I then examined the roles of regulatory elements in the locus, including using a machine learning approach to probe the mechanisms underpinning their interactions with the Vκ region. Furthermore, I have proposed …
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Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… genes that are clustered in the genome into three distinct loci (Keratin type I/II loci and Epidermal Differentiation Complex (EDC) during differentiation of the epidermis are poorly understood. By using 3D-Fluorescent In Situ Hybridization (FISH), we determined that in the …
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The subnuclear localisation of Notch responsive genes
… the nucleus is important for its regulation and genome reorganisation can occur following gene activation or during development. Therefore this project aimed to label individual Notch responsive loci and determine their subnuclear localisation. In order to tag loci of interest a CRISPR/Cas9 …
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