Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"genome maintenance"”.
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Regulatory of genome maintenance
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01
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Single molecule studies of processes involved in genome maintenance
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01
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Mechanisms underlying genome maintenance and tumour suppression by BRCA2
… recombination, replication stress tolerance, maintenance of the G2 and spindle assembly checkpoints, and in completion of cytokinesis. Much evidence suggests that BRCA2 acts as a tumour suppressor by maintaining genome integrity through its functions during DNA replication, repair and …
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Investigating the role of NSMCE2 in cell cycle progression and genome maintenance
Preserving genome integrity is vital for protection against the development and progression of cancer. The SMC5/6 complex is an evolutionarily conserved protein complex thought to be involved in this process, specifically its SUMO ligase NSMCE2 subunit. Prior to this thesis, CRISPR-Cas-9 mutant …
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New Insights into the Roles of FAN1 Nuclease in Genome Maintenance and Disease
… FAN1 and SNM1A participate together in critical genome maintenance activity where the FA-pathway is insufficient. In addition to driving KIN, variants in FAN1 have also been associated with earlier onset of Huntington's disease (HD), a trinucleotide repeat expansion disease. Depletion of FAN1 …
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Structure/function analyses indicate novel roles for mycobacterial DnaQ homologs in genome maintenance
… of DnaQ and DnaQ-UvrC, and their contribution to genome dynamics in a family of organisms that includes major human and animal pathogens.
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Designing Novel DNA-Binding Proteins with Generative Deep Learning
… processes, such as gene regulation and genome maintenance. Designing protein backbones specifically tailored for DNA binding remains a challenging task, requiring the exploration of novel computational approaches. This thesis presents a novel framework for gen- erating protein backbones …
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Safeguarding the mitochondrial genome: identifying components of the mtDNA repair machinery
… the molecular processes underlying mitochondrial genome maintenance. The project initially utilised a candidate-driven approach, focusing on REC and its interacting partners. REC, the Drosophila ortholog of human MCM8, is an MCM helicase that is known to be involved in meiotic recombination. …
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The Role of E2F1 In The Response to Dna Double Strand Breaks
… which may directly promote DNA repair and genome maintenance.</p>
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Effect of Werner and Bloom Helicase Deficiencies On Dna Double-Strand Break Repair
… shown that these two helicases do play a role in genome maintenance. However, specific roles in DSB repair are still unclear. This study focuses on WRN's role in nonhomologous end-joining (NHEJ) and BLM's role in both NHEJ and homologous recombination (HR). This study is unique in that DNA repair …
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ZSCAN4 AND REPLICATION STRESS: SAFEGUARDING GENOME STABILITY IN MOUSE EMBRYONIC STEM CELLS
… of DNA replication is essential for maintaining genome stability. However, replication can be disrupted by both endogenous and exogenous factors, leading to a condition known as replication stress (RS). RS slows down and eventually stalls replication forks. If stalled forks are not resolved, they …
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ATR SIGNALING IN GENOME REPLICATION AND MAINTENANCE
… response that is essential for maintaining genome integrity. Over the last few decades, ATR’s role in controlling the checkpoint upon the DNA damage response has been well studied. However, most studies of ATR function have relied on a few well-established substrates, and we still lack a …
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Regulation of Mitochondrial Function by Histone Acetylation and Mitochondrial Acetyl-CoA Levels in Saccharomyces cerevisiae
… mediated by Msn2/4 in starving cells. Recent genome-wide screening identified several mutants displaying significantly enhanced levels of both chaperone reporters (pHSP26-HSP26-RFP and pSSA3-RFP), including the mutants of the HDA1 histone deacetylase (HDAC) complex, suggesting the involvement …
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The Role of Esc2 and DNA in Smc5/6-mediated Sgs1-Top3-Rmi1 Sumoylation
… of cells and requires precise copying of the genome, which is challenged by many obstacles or damages such as DNA double-strand breaks (DSBs). There are several mechanisms to deal with DSBs and replication blockages, including homologous recombination (HR). HR leads to the formation of DNA …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
… that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present mechanical studies of E. coli SSB bound to ssDNA using high-resolution …
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Genomic Instability and the Oncohistone H3K27M Drive Gliomagenesis in a Murine Model
<p>Maintaining genome stability is crucial for human health and it is of particular importance in neural cells during early brain development. Genome maintenance occurs at two broad stages; surveillance during DNA replication and DNA damage repair in differentiating and mature cells. Neural cells …
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… on two distinct areas that are important for genome stability. Both areas focus on DNA repair pathways that require the action of nucleases, specifically Exonuclease 5 and Ribonuclease H2. First, I describe the biochemical and molecular characterization of the novel Exonuclease 5 family of …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… epigenetic regulators and their role in genomic maintenance. For this thesis work, I have developed a biophysical platform to study chromatin-associated factors at the single-molecule level (Chapter 2). This methodology allows us to extract key mechanistic details often obscured by standard bulk …