Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 49 for “"genome engineering"”.
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Genome engineering in saccharomyces cerevisiae
… titer was improved through pathway-based engineering, such rational design often meets with great challenges in cellular reprogramming due to our limited knowledge of complex biological systems. Directed evolution, on the other hand, has been proved as a better strategy by performing …
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Algorithms for E. coli genome engineering
… recombineering is one of methods of performing genome engineering. However, this method of genome editing is not very specific and efficient and is highly dependent on the genomic regions that are targeted (integration sites). In this project we explored ways of identifying what makes a site …
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Site-Specific Genome Engineering in Mouse Primary Fibroblasts
Site-specific genome engineering is a powerful tool for medical therapeutics and basic scientific research. As the name implies, site-specific genome engineering describes the ability to add or subtract nucleic acid information in a precise, controlled manner within the genome. This technology has …
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Site-Specific Genome Engineering in Mouse Primary Fibroblasts
Site-specific genome engineering is a powerful tool for medical therapeutics and basic scientific research. As the name implies, site-specific genome engineering describes the ability to add or subtract nucleic acid information in a precise, controlled manner within the genome. This technology has …
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Mammalian genome engineering: Fundamental investigation and method development
… it possible to efficiently engineer mammalian genomes. Understanding the genome-search mechanism of structurally distinct TALEN and CRISPR/Cas9 at the molecular level will not only increase our fundamental understanding of DNA-binding proteins but also enable improvement of these powerful …
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
… knock out a gene. The potential for such precise genome editing would reduce worry about insertional mutagenesis or misregulation, as only the specific gene under its native promoter would be targeted. Thus, LHEs have drawn intense interest for their research, biotech and clinical applications. …
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Advancements in genome engineering and applications in mammalian systems
… development and applications in the field of genome engineering. From zinc finger proteins (ZFPs), to transcription activator-like effectors (TALEs), to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), these programmable DNA targeting …
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Interrogation of CRISPR-Cas targeting specificity for mammalian genome engineering
… DNA endonucleases enable high efficiency genome editing across the biological diversity for research, industrial, and biomedical applications. Human genome editing with CRISPR-Cas just recently made its debut in human clinical trials and has immense therapeutic potential to fix …
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Synergy of protein and genome engineering for fuels and chemicals production
… value-added compounds from biomass, significant engineering work has to be performed to optimize naturally occurring organisms with better catalytic properties. The rapid advancement in understanding of molecular mechanisms of biological functions as well as development of novel molecular …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… mitochondrion are encoded in the nuclear genome. However, the mitochondrial genome, which is a circular, multi-copy genome of roughly 17 kb in size, also encodes for 13 polypeptide genes that form key components of the OXPHOS complexes, along with the 22 tRNA genes and 2 rRNA genes …
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Better safe than sorry: new CRISPR/Cas9 tools for improved genome engineering
… nucleases are efficient tools to edit cellular genomes in a variety of organisms. However, the in vivo application of this technology is still severely limited by unwanted genomic cleavages, that are further increased by long-term expression of the nuclease and can lead to unpredictable results. …
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Genome engineering and functional gene regulation tools for the study of malaria parasites
… regulation of native loci. This work combines genome-engineering technologies and regulatory systems designed to provide a robust platform for the identification and characterization of essential functions in human malarial parasites.
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Artificial neural network and precision genome engineering frameworks for genetic system engineering in mammalian cells
Synthetic biology is an emerging engineering discipline that merges biology with engineering design principles with the objective to engineer novel biological behavior in living organisms. The engineering process in synthetic biology, however, is often unpredictable and inefficient. Therefore, …
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A rapid, flexible and scalable DNA assembly platform for genome engineering and regulated gene expression applications in Plasmodium falciparum
… by difficulties in manipulating the parasite's genome and functionally perturbing gene expression in a controlled way. Our lab has developed inducible systems to control P. falciparum gene expression, and has achieved successful editing of the P. falciparum genome using CRISPR/Cas9 technology. …
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An expanded search for RNA-programmable genomic engineering effectors
… single effectors, akin to the revolutionary genome-engineering tools Cas9 and Cpf1. Whereas previous search strategies relied on protein proximity to CRISPR-associated spacer acquisition proteins Cas1 and Cas2, this approach was seeded on CRISPR arrays alone. What resulted was the discovery …
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Rewriting the genome of Escherichia coli
… scale is opening the door to writing entire genomes; this constitutes a powerful approach to address fundamental biological questions, and may enable the creation of designer organisms with useful properties. One interesting avenue for investigation is the creation of recoded genomes, where …
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Engineering Disease Resistance in a Reservoir Species for the Mice Against Ticks Project
This thesis explores the application of genome editing technologies to combat zoonotic infectious diseases through the development of a novel heritable immunization strategy targeting reservoir species. Focusing on Lyme disease, where white-footed mice (Peromyscus leucopus) serve as the primary …
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Design and debugging of ultrastable engineered genetic systems
… to the controlled production of chemicals. Engineering such genetic systems is challenging as these genetic systems often consist of multiple genes and genetic parts (>4 genes or > 45 genetic parts) interacting with each other as intertwined networks. These intertwined networks are …
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Molecular interventions to overcome the ageing of CNS stem cells
… systemically delivered, cell-type-specific, genome engineering technique to perturb age-related genes in adult rodents. The work presented in this thesis provides a new understanding of OPCs in homeostasis and in ageing. By identifying multiple interconnecting signalling-pathways by which …
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A novel CRISPR-Cas9 platform with divergent targeting capabilities
… of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. The range of sequences that CRISPR endonucleases can recognize, however, is constrained by the need for a specific protospacer adjacent motif (PAM) flanking the …
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