Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 171 for “"genome editing"”.
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Discovery of novel systems for genome editing
Genome editing techniques allow for targeted modifications to specific genes, enabling the correction of genetic mutations that cause diseases, as well as the functional study of specific genes. The capacity to precisely modify genes at desired loci offers unprecedented opportunities in therapeutic …
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Sculpting 3d Chromatin Folding Via Genome Editing
… inserts revealed their distinct contributions to genome folding. Altogether, short DNA insertions can suffice to shape the spatial genome in a manner influenced by chromatin context. Additionally, we frame our findings in the context of recent advances in experimentally creating chromatin loops, …
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Expanding the genome editing toolbox for biomedical applications
… transgene-delivery methods has revolutionized genome editing in mice. One such strategy is CRISPR-READI, which combines adeno-associated virus (AAV)-mediated gene delivery with electroporation of Cas9-RNPs. The AAV delivers transgenes (up to 4.7 kb) as ssDNA to the zygotes, which is followed by …
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Development of genome editing in potato and tobacco
… Gene silencing can be achieved through gene editing, a method that allows precise alterations to an organism’s DNA (Zhang et al., 2017). Over the past decade, genome editing tools have been developed which enables targeted genetic modifications (Alamillo et al., 2023). One of these tools, …
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Characterization and Engineering of Transposons for Genome Editing
Since the human genome was sequenced in 2000, we have rapidly elucidated previously unappreciated associations between our genetic code and human disease. Driven by the rapid development of new molecular technologies, there has been increased desire to treat these genetically rooted diseases by …
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Multiplex genome editing and gene regulation in eukaryotic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01
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Understanding antigen processing in chickens using genome editing technology
… of each DM gene are unknown. In this thesis, the genome editing approach of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 was used to begin to understand the peptide loading systems in the chicken. For the first time, the CRISPR-Cas9 system was optimised and implemented …
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Applications of genome editing for disease modeling in mice
The genome holds the blueprint of life and heredity. In the case of the mammalian genome it is comprised of billions of DNA bases grouped into elements that we are beginning to understand (ie genes) and others we know little about (ie noncoding DNA). Forward and reverse genetics in cells and animal …
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Genome editing for engineering resilience to climate changes in grapevine
In Arabidopsis stomatal initiation relies on the transcription factor SPEECHLESS, which is positively regulated by AtEPFL9, a small peptide of the Epidermal Patterning Factor family. In grapevine, two EPFL9 paralogs exist, VviEPFL9-1 and VviEPFL9-2 but despite a structural similarity, their …
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Methods to increase the efficiency of precise CRISPR genome editing
… Ausgangszellen untersucht werden. Da die Genome unserer nächsten ausgestorbenen Verwandten Neandertaler und Denisovaner aus konservierter DNA in alten Knochen sequenziert wurden, könnten ebenso Unterschiede zwischen Mensch und diesen Spezies oder dem letzten gemeinsamen Vorfahren …
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Breeding broccoli resilient to multi-abiotic stresses using genome editing
… senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. …
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Exploration of methods for many-site genome editing with MAGE
… codes, modifications must be made throughout the genome wherever the affected codon is used. Making such numerous and widespread genomic changes requires specialized techniques. MAGE is well-suited to this task, being highly amenable to multiplexing and having low time and resource costs per site. …
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Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing
… CRISPR/Cas system has been applied for mammalian genome editing because it provides site-specific DNA double-stranded breaks with simplicity and precision. In this study, I demonstrate the feasibility of using CRISPR/Cpf1 to correct a DMD exon 48-50 out-of-frame deletion mutation in cardiomyocytes …
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Natural and Exogenous Genome Editing In Wiskott-Aldrich Syndrome Patient Cells
… virus-free zinc-finger nuclease (ZFN)-mediated genome editing strategy for correction of <em>WAS</em> muations. We generated induced pluripotent stem cells (iPSC) from skin fibroblasts of a WAS patient carrying an insertional frame-shift mutation. Subsequently, a WAS-2A-eGFP transgene was …
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DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
The ability to manipulate complex genomes in a precise manner is essential for studying biological processes in model systems, engineering plant strains for agriculture, or advancing human cellular therapies to treat diseases. Genomic alterations are most efficient when a double-strand DNA break is …
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Genomic Characterization of Sickle Cell Mouse Models for Therapeutic Genome Editing Applications
… Previously, we showed that CRISPR-Cas9-mediated genome editing can recreate a naturally occurring HPFH variant in the γ-globin (HBG1 and HBG2) promoters. Disruption of a TGACC nucleotide motif within this region by Cas9-mediated non-homologous end joining in human erythroid cells or their …
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GENOME EDITING, A NEW BIOTECNOLOGICAL APPROACH TO PRODUCE LYCOPENE-RICH PIGMENTED ORANGES
… stato adottato un approccio di tipo “CRISPR/Cas9 genome editing”, per cercare di ottenere frutti di agrumi arricchiti in entrambi i pigmenti. Inizialmente, sono stati valutati i diversi livelli di espressione genica della fitoene sintasi (PSY), beta ciclasi 2 (β-LCY2) e GTP binding-protein (geni …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
In the post-genome era, one of the most important topics of research is to edit or program genomic sequences and to generate desired phenotypes. Although virus-based strategies have long been developed to for efficient gene insertion, the random or semi-random integration can disrupt certain …
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Genome editing with CRISPR-Cas9 in the Illinois long term selection experiment
Recent advances in genome editing by Clustered Regularly Interspaced Palindromic Repeat (CRISPR) and CRISPR Associated Protein 9 (Cas9) have markedly increased our ability to characterize genes and use genetics to the benefit of agriculture. In this work, we utilize this technology to study the …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… a special section reviews the emerging genome editing technology CRISPR/Cas system in these organisms together. Chapter 2 and Chapter 3 focus on one of the most important intracellular activities, nuclear localization of proteins, and describe the characterization of nuclear localization …
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