Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"genome doubling"”.
-
Whole genome doubling confers unique genetic vulnerabilities on tumor cells
Whole genome doubling (WGD) generates genetically unstable tetraploid cells that fuel tumorigenesis. Cells that undergo WGD must acquire adaptive characteristics to accommodate their tetraploid state, and these adaptations may confer unique vulnerabilities that can be exploited therapeutically. We …
-
Using spontaneous haploid genome doubling to access favorable alleles in exotic maize (Zea mays L.) germplasm
… improvements of DH production over the years, genome duplication is still a major bottleneck. Its efficiency tends to be reduced when the technology is applied in exotic germplasm. The use of spontaneous haploid genome doubling (SHGD) can improve DH production, including in exotic germplasm. In …
-
Determining how variations in cell and nuclear size contribute to mitosis and tumorigenicity in cancer cells that undergo whole genome doubling
Whole genome doubling (WGD) is a frequent event in human tumors associated with metastasis and poor prognosis. The genetic redundancy afforded by WGD is thought to attenuate the deleterious effects of gene mutations and chromosome missegregation, thereby enabling the propagation of genomic and …
-
Analysis of alterations in the human cancer genome
… alterations in a cancer sample in units of genomes (DNA mass). The meaning of such measurements is highly dependent on the tumors purity and its overall ploidy; they are hence complicated to interpret and compare across samples. Ideally, copy-number should be measured in …
-
A Pan-Cancer Single-Cell Analysis of Intratumoral Copy Number Diversity and Evolution
… evolution (PCNE), a short period of transient genome instability led to the generation of many tumor subclones (7-22) that grouped into 3-5 major superclones.</p> <p>We then extended the application of ACT to a pan-cancer study involving 94 tumors across seven major cancer types: bladder, …
-
Telomere-Driven Tetraploidy and its Relevance to Cancer
… argues that tetraploidization, i.e. whole genome doubling, followed by chromosome loss is likely to represent an early event in the development of subtetraploid tumors. The origin of tetraploidization in cancer is still unclear. Here we de- scribe a new potential mechanism of …
-
Multi-omic characterisation of Barrett’s oesophagus reveals a molecular continuum in the progression to oesophageal adenocarcinoma
… ERBB2 amplification, APC mutation and whole genome doubling are confined to cases that have progressed with TP53 being by far the most prevalent. In contrast CDKN2A alteration occurs early in around 50% of indolent cases. SV analysis reveals a dominance of translocations from the early ND …
-
Reconstructing Mutational Lineages In Breast Cancer By Multi-Patient-Targeted Single Cell Dna Sequencing
… with early mechanisms of genomic instability, genome doubling, and aneuploid transformation. Single cell DNA sequencing (scDNA-seq) methods are powerful tools for profiling these mutations in cancer cells, however most genomic regions sequenced in single cells are non-informative. To overcome …
-
Cytogenetics and genome structure in genus Miscanthus, a potential source of bioenergy feedstocks
… This study (chaper 2) combines information on genome size and ploidy levels and DNA sequence variation to classify Miscanthus accessions to aid potential biomass crop improvement programs and to investigate the evolution of the genus. We observed that Miscanthus accessions fell into 4 groups, …
-
Investigating the origins of polyploidy in Barretts oesophagus and oesophageal adenocarcinoma
… of polyploidy after p53 loss via an unknown genome doubling event. This increase in genomic material is likely to contribute to OEAC evolution and diversification. To identify the origin of polyploidy in OEAC, I analysed mitosis in both BE and OEAC cell lines by time-lapse imaging and …
-
Integrating Epidemiological and Genomic Factors to Inform Outcomes in Barrett’s Oesophagus and Oesophageal Adenocarcinoma
… available clinical, epidemiological and whole-genome sequencing data was also examined. There was little to no association between most of the 34 clinical and epidemiological factors and the OAC phenotypes. Weak associations were observed for cigarette smoking and gender with self-reported …
-
Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… Correct estimation of the ploidy of single cell genomes is crucial for many aspects of downstream analysis, such as copy number calling and inference of cell phylogenies. Based only on single-cell DNA sequencing information, scAbsolute achieves accurate and unbiased measurement of single-cell …