Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 93 for “"genetic tools"”.
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Leveraging HSF1 chemical-genetic tools to elucidate mechanisms of proteostasis
… work has focused on applying these HSF1 chemical-genetic tools to approach various problems in the field of metazoan proteostasis. First, I examined the interplay between stress response pathways regulating chaperone expression and post-translational modification by the small ubiquitin like …
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Dissection of spatiotemporal intracellular signaling using engineered chemical and genetic tools
… thesis describes the development of chemical genetic tools that specifically control individual signaling nodes in a cell using three distinct approaches. The first approach utilizes conformation-selective inhibitors that modulate the allosteric regulation of Src Family Kinases (SFKs). SFKs …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… In addition, the relatively low genetic diversity in soybean relative to other major crops (e.g., corn) limits the available phenotypic variations that can be exploited for discovering genes, gene function and crop improvement. Thus, developing a feasible, efficient molecular …
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Design and construction of two genetic tools for use in mycoplasmas
<p>http://www.worldcat.org/oclc/44187295</p>
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Brook Trout Population Genetic Tools for Natural Barriers in Fragmented Subwatersheds
… was to investigate applications of population genetic analysis tools as indicators of barrier effects on brook trout populations in fragmented subwatersheds. In chapter1, I tested the hypothesis that brook trout population genetic differentiation (FST) above and below barriers will differ in …
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Using genetic tools to understand the population ecology of stream fishes
… and beneficial, will affect persistence. I used genetic tools to further this understanding in three case-study fish species of the southeastern United States: Roanoke logperch (Percina rex) of the greater Roanoke River basin and redline (Etheostoma rufilineatum) and greenside darters (E. …
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Application and Development of Advanced Genetic Tools to Study Adult Stem Cells
… Title: Application and Development of Advanced Genetic Tools to Study Adult Stem Cells By: Amanda Andersson Rolf In adult mammals, the gastrointestinal (GI) epithelium exhibits the highest turnover rate among the endodermal tissues. The harsh luminal environment of the GI tract necessitates …
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Electrochemical and genetic tools for analysing and engineering bioenergetic processes in cyanobacteria
… transport chain. Furthermore, the available tools for analysing and engineering cyanobacterial electron transport are scarce compared to those for model plant and green algal species. This thesis addresses this research challenge by developing a range of electrochemical and synthetic biology …
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The Development of Genetic Tools for Protein Characterization and Genetic Engineering in the Acetic Acid Bacterium Gluconobacter Oxydans
… use and the improvement of strains via genetic engineering has the potential to produce novel products as well as increase the yields. However, genetic manipulation in G. oxydans is often difficult and time-consuming because few genetic tools are available for use in this organism. To …
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The use of ecological studies, genetic tools, and citizen science to asses the biological invasion status of green seaweed Codium fragile (Suringar) Hariot 1890 in the southern area of the Bay of Biscay (Cantabrian Sea)
Codium fragile subsp. fragile Suringar (Hariot) 1890 está catalogado como uno de los 100 peores invasores de Europa. Cinco especies de Codium se registran en la fauna intermareal marina a lo largo de la costa cantábrica, como nativas (C. tomentosum, C. decorticatum, C. vermilara y C. adhaerens) y …
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Unexpected metabolic versatility discovered using new high-throughput genetic approaches in a model methanogen
… challenges in culturing and adaptation of genetic tools have slowed our understanding of the mechanisms essential for survival in the absence of oxygen. My thesis research has expanded and advanced the application of the genetic tools available for methanogens and furthered our …
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Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were …
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Identification of a novel autonomous role for Ecdysone Receptor during Drosophila ovarian germ cell differentiation
… self-renewal; however, due to lack of compatible genetic tools, potential autonomous roles in the germline have not been fully evaluated. Ecdysone elicits a diverse array of transcriptional responses by binding to a heterodimeric complex composed of Ecdysone Receptor (EcR) and Ultraspiracle (Usp). …
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Reconstruction of major male and female lineages of the Strand Muslim community
… The Study has shown the ability of molecular genetic tools to give insight into the origins and history of local communities. The study was also used as a point of reference for the Strand Muslim Community project. Genetic variations of the Y-chromosome and mitochondrial DNA for the pilot …
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Genetic Analysis of Adipose Lineage and Development
… development in vivo because of the lack of genetic tools. To this end, I generated a few knock-in mice that offer both spatial and temporal controls to manipulate gene expression in adipose tissues. Here I demonstrate the application of one of the tools, PPARgamma tTA, in exploring some …
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Enabling Cl-Based bioconversion with metabolic engineering
… poorly characterized biochemically, and limited tools exist for their genetic modification. This leaves the metabolic engineer with a choice: to develop genetic tools to enable engineering in the desired host, or to import the relevant catabolic pathway into a more tractable organism, such as …
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Metabolic Engineering of oleaginous yeast for the production of biofuels
… as the host organism. We establish new genetic tools for engineering Y. lipolytica beginning with an expression vector utilizing the genetic features from translation elongation factor 1-a (TEF). Additionally, a complementary plasmid was developed allowing for multiple plasmid …
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Phylogeographic and environmental DNA analysis of the mudpuppy (necturus maculosus maculosus)
<p>Understanding species’ genetic diversity allows for informed management that preserves evolutionary potential. Informed management becomes crucial as genetic diversity declines and species become susceptible to threats such as climate change. For some species, it can be difficult to determine …
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Neural Regions Affecting Female Aggression and Receptivity in Drosophila melanogaster
The genetic tools that exist in Drosophila melanogaster make it possible to assess the influence of specific regions of the brain on complex behaviour. Examples of such behaviours include female aggression and receptivity to male courtship. Silencing a candidate region called the mushroom body (MB) …
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Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies
… to culture H. felis and use more common genetic tools. A renewable source of H. felis DNA in the form of a large insert library was created to study the genome and search for genes related to pathogenicity and survival. The genome size of H. felis was determined to be 1200 kb using pulsed …
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