Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"genetic stability"”.
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Genetic Stability of a Genetically-Engineered Chimeric Porcine Circovirus (PCV) Vaccine, PCV1-2
… the immunogenic capsid protein of PCV2 into the genetic backbone of the non-pathogenic PCV1. This chimeric PCV vaccine, called PCV1-2, was shown to induce protective immunity against PCV2 infection in pigs. The vaccine is currently on the market in a killed form. In order to develop a live …
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LACTICASEIBACILLUS PARACASEI DSM 34154 (L. CASEI DG®) IN PRECLINICAL AND CLINICAL STUDIES AND ITS GENETIC STABILITY OVER A DECADE OF INDUSTRIAL PRODUCTION
… of the whole PhD thesis, which investigates its genetic stability over a decade of industrial biomass production, as well as its effects in preclinical and clinical studies. LC-DG, a well-characterized probiotic strain, has been included in the dietary supplement Enterolactis® on the market for …
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Evaluation of Genetic Diversity and Stability of Colocasia Cultivars Using ISSR
… (Nacogdoches, TX), cultivar assessment, genetic diversity, stability, and relationship were examined with banding patterns produced from Inter-simple sequence repeat (ISSR) markers. Comparing banding patterns of vegetatively propagated clones of each cultivar, genetic stability was …
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Construction, stability and immunogenicity of recombinant BCG expressing HIV-1 subtype C gag under the control of MtrA promoter, with or without the leader sequences
… This was done to determine whether the genetic stability of the recombinant mycobacteria could be improved by modification of these vector features and to gain insight into what types of immune responses may be elicited in mice.
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Genetic and epigenetic regulation of the cell wall glycoproteins, Flo10p and Flo11p, generate phenotypic variation in S. cerevisiae
… Such phenotypic variation can result from genetic regulation, in which changes in DNA sequence produce the variant phenotype, or epigenetic regulation, in which there are no changes in DNA sequence associated with the variant phenotype. This doctoral thesis describes the identification and …
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Discovering the p53 isoforms’ code
p53 has a key role in the maintenance of the genetic stability and, thus, in preventing tumour development. The current model is that the p53-dependent responses are solely driven by p53α. The uncovering that the TP53 gene physiologically expresses several p53 isoforms, challenges this paradigm. …
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Characterization of a Low-Fidelity Live-Attenuated Vaccine for Venezuelan Equine Encephalitis Virus
… vaccines, this has implications for stability, as replication may result in mutations that either restore the wild-type phenotype via reversion or compensate for the attenuating mutations by increasing virulence (pseudoreversion). Recent studies have demonstrated that altering the …
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Investigating the role of non-structural genes in the genotype and phenotype of yellow fever vaccine strain 17D
… The impact of the introduced substitutions on genetic diversity, multiplication kinetics, sensitivity to the antiviral Ribavirin, and attenuation in an AG129 mouse model were evaluated. 17D-204 virus is known to have reduced genetic diversity, increased multiplication kinetics in A549 cells, …
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Directed evolution of antimutator E. coli
… address this problem is to engineer evolutionary stability by lowering a host organism’s basal mutation rate and concomitantly reducing the probability that an encoded function will become mutated. In Chapter 1 of this dissertation, I discuss the nature of the metabolic cost associated with …
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Cell cycle checkpoints and checkpoint kinases in the apicomplexan parasites Plasmodium and Toxoplasma
… are essential to maintaining growth and genetic stability in many other eukaryotic cells. Whether these checkpoints are as essential in apicomplexan parasites, which have very divergent forms of replication, needed to be explored. Phosphatidylinositol 3-kinase-related kinases (PIKKs) are …
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Investigating the Epigenetic Mechanism Behind Transgenerational Inheritance in Mice with Abnormal Folate Metabolism
… germ cell morphology and function abnormalities, genetic instability and altered germ cell epigenetic patterns. We first considered if the $\it{Mtrr^gt}$ mutation affected testes morphology, spermatogenesis or sperm parameters. These were largely normal in $\it{Mtrr^gt}$ males. Next, we performed …
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Mechanisms of cell damage and recovery in cryopreserved freshwater protists
… of cultures and effectively guarantees genetic stability. To improve post-thaw viability levels and to preserve freeze-recalcitrant organisms, components of the cryogenic process which predispose and/or subject cells to stress and injury require to be determined. In this study markers of …
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Investigating the genomic, transcriptomic and metabolic landscape of Chlamydomonas reinhardtii in the context of vitamin B12 availability
… require B12 as an enzyme cofactor. The phylogenetic distribution of B12 auxotrophy across the eukaryotic tree of life suggests that this trait has evolved multiple times in the algal kingdom, indicating that it has a selective advantage of some sort. Indeed, previous artificial evolution …
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Animal and Plant Viruses with Double-Stranded Ribonucleic Acid
… and also with respect to the large amount of genetic material in the virus particle, reovirus is unique among animal viruses containing RNA. There is a second virus, the wound tumor virus, that has the same size and structure as reovirus. This plant virus produces tumors in sweet clover and …
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Tropical bracken, a powerful invader of pastures in South Ecuador: Species composition, ecology, control measures, and pasture restoration
… Analysis of heterozygosity indicated a higher genetic stability of the diploid P. arachnoideum population as compared to the allotetraploid P. caudatum population. Spatial extension of the individual clones is much smaller than reported for the northern bracken, indicating higher significance …