Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 59 for “"genetic screening"”.
-
Optimization of molecular tools for high-throughput genetic screening
Forward genetic screening allows for the identification of any genes important for a particular biological process or phenotype. While the power of this approach is broadly agreed on, the efficacy of currently available tools limits the strength of conclusions drawn from these experiments. This …
-
In Vivo Genetic Screening to Identify Mechanisms of Resistance to Cancer Immunotherapy
… we developed a pooled, loss-of-function in vivo genetic screening approach using CRISPR/Cas9 genome editing in mouse transplantable tumors treated with vaccination and PD-1 checkpoint blockade. We optimized the expression of CRISPR genome-editing components in immunocompetent mice and screened …
-
Preparing for Personalized Medicine: Improving Genetic Screening Resources for Primary Care Providers
Due to the expansion of genetics testing and the current strain on the genetics services workforce, Primary Care Providers (PCPs) will have to play a growing role in the management of of patients with genetics needs. However, PCPs do not have the knowledge or support to take on this role. This …
-
Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… technology in primary T cells and to optimise screening approaches to identify regulators of CTL killing. The first half of the thesis focuses on primary mouse CTL. The CRISPR technology was successfully optimised in CTL using Cas9-ribonucleoprotein complexes resulting in efficient …
-
Xenopus laevis as a chemical genetic screening tool for drug discovery and development.
… the applicability of the X.laevis chemical genetic screening model towards drug discovery and drug development. The NCI diversity set II compound library was screened to identify abnormal pigmentation generating phenotypes that may have therapeutic application towards the treatment of …
-
Attitudes to ward and Uptake of Prenatal Genetic Screening and Testing In Twin Pregnancies
… prenatal testing, interest in and uptake of genetic screening and testing in twin pregnancies has not been investigated. Therefore, this study aimed to describe the attitudes toward and uptake of current prenatal genetic screening and testing options in twin pregnancies. Forty-two women with …
-
Psychological and Sociodemographic Predictors of Psychological Distress in BRCA1 and BRCA2 Genetic Testing Participants within a Community Based Genetic Screening Program
… of developing breast, ovarian or other cancers. Genetic testing for mutations in these two genes has recently become commercially available. There have been varying amounts of psychological distress noted among women with a family history of breast cancer. Distress has been observed to impact …
-
Genetic dissection of EGFRvIII brain and spinal mouse gliomas through whole-exome sequencing and in vivo piggyBac mutagenesis forward genetic screening
… significant morbidity in children; however the genetics of these spinal gliomas is poorly understood. EGFRvIII is a common driver mutation in brain gliomas; it is unclear when this is acquired during glioma evolution and what its cooperative genetic drivers are. Here, we show that EGFRvIII …
-
Prenatal Screening Decision-Making Facilitated Through An Online Patient Education Module
<p>As recommendations for prenatal genetic screening are expanded to include patients with pregnancies at low risk for aneuploidy, there is a need to develop accessible mechanisms to promote informed decision-making about genetic screening. The use of patient decision aids has been shown to promote …
-
Evaluating Triage to Genetic Counseling Using an Online Reproductive Genetics Module
… of Obstetricians and Gynecologists recommends genetic screening for all pregnant women. As clinical recommendations broaden and demand for prenatal screening increases, obstetric practitioners report time constraints and lack of genetics knowledge as challenges to providing sufficient pretest …
-
Functional analysis of amyotrophic lateral sclerosis mutations using Drosophila cell culture models
… combines molecular biology, high-throughput genetic screening, and transcriptomics analysis to identify potential therapeutic targets of ALS. Transient Drosophila S2R+ cell models expressing wild-type or mutant forms of the ALS-associated genes FUS, SOD1, TDP43 were generated to evaluate …
-
UNRAVELLING THE MOLECULAR BASIS OF ALZHEIMER¿S DISEASE AND FRONTOTEMPORAL DEMENTIA: GENETIC AND EPIGENETIC APPROACH THROUGH NEXT GENERATION SEQUENCING AND OPENARRAY TECHNOLOGIES
… complex heterogeneous disorders with a strong genetic background, but the identification of a genetic cause is difficult given the multifactorial aetiology of the disorders. Epigenetic and environmental factors interplay to influence this complexity. The aim of the present project was to …
-
Development and application of a general method for somatic cell genetics
Genetic analysis, or genetic screening, is an efficient method for identifying the set of genes involved in a biological process. In microorganisms, powerful techniques enable systematic loss-of-function screens, which have yielded critical insights into the molecular basis of many fundamental …
-
Regulation Of Acute Myeloid Leukemia Cell Proliferation By E3 Ubiquitin Ligase DCAF15 Control Of Cohesin Dynamics
… DCAF15 remains unknown. Using a domain-focused genetic screening approach, we reveal DCAF15 as an acute myeloid leukemia (AML)-biased dependency. Loss of DCAF15 results in suppression of AML through compromised replication fork integrity and consequent accumulation of DNA damage. Accordingly, …
-
Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… 400 kb, 250 kb and 100 kb, respectively. Genetic screening of an AN12 transposon insertion library showed that two megaplasmids, pREA400 and pREA250, are conjugative. It is known for other bacterial systems that a relaxase encoded by the traA gene is required to initiate DNA transfer …
-
Characterization of endocytic escape pathway and its role in adaptive immunity
… of proteins, the Kozik lab has established a genetic screening strategy where escape of a ribosome-inactivating protein, called saporin, is being followed. When saporin enters the cytosol, it causes translational arrest quantified using puromycin labelling of newly synthesized polypeptides. …
-
Mechanisms and impact of alternative transposition-induced segmental duplications
… duplications in maize. We applied a variety of genetic, molecular, statistical and bioinformatics approaches, including genetic screening, PCR, Southern blotting, qRT-PCR, microarray, mRNA-sequencing, small RNA-sequencing, and a self-developed program (STRAND: Search for Transposon-Induced …
-
Genetic Analysis of The Hippo Pathway In Mouse Liver
… and tumor treatment remain unsolved puzzles. Genetic screening for tumor suppressor genes in <em>Drosophila</em> revealed the Hippo-signaling pathway as a kinase cascade consisting of five core components. Disrupting the pathway by deleting the main component genes breaks the balance of cell …
-
Greenhouse growth of Pinus x rigitaeda seedlings in response to water stress and correlations with 7 year plantation performance
… measured. Water stress also elicited some genetic differences in the pitch pine and loblolly pine that were not apparent under the non-stressed treatment. The major differences between pitch and loblolly seedlings seemed to be primarily a function of large differences in seed size. Free …
-
Translational mechanisms of stem cell fate regulation in epidermal oncogene tolerance
… Furthermore, by coupling ribosome profiling with genetic screening, we provide direct functional evidence that RAS-induced differentiation is driven by eIF2B5-mediated translation of ubiquitination genes. We reveal ubiquitin ligase FBXO32 as a promoter of epidermal differentiation with no effect …
Page 1 of 3