Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"genetic mouse model"”.
-
Understanding Autism Pathology: Insights from Genetic Mouse Model Manipulation of KCTD13 and SHANK3
… brain. We report that our Kctd13 deletion mouse model results in decreased synaptic transmission (input/output curves and mEPSC and mIPSC frequency) in area CA1 of the hippocampus with normal paired-pulse ratio, normal mEPSC/IPSC amplitude, and no change in another correlate of presynaptic …
-
Aberrant assembly and function of a hippocampal circuit in a genetic mouse model of schizophrenia
Schizophrenia is highly heritable yet very few genetic risk variants have been unequivocally linked to the disease. Disrupted in Schizophrenia 1 (DISC1), was first discovered in a family with a balanced translocation t (1; 11) (q42; q14) and a history of psychiatric disease that segregates with the …
-
Investigation of CD4+ T cell heterogeneity and function in a genetic mouse model of lung adenocarcinoma
… CD4+ T (Tconv) and Tregs in an inducible genetic mouse model of lung adenocarcinoma. We show that lung tumor-associated Tregs have a distinct transcriptional signature that is associated with survival in multiple cancers. Furthermore, we find that tumor-associated Treg and Tconv cells are …
-
Investigation of endogenous and adoptively transferred T cell function in a genetic mouse model of lung adenocarcinoma
… T cell responses. Using an autochthonous mouse model of lung adenocarcinoma, we longitudinally profiled CD8⁺ T cells from the lungs of tumor bearing mice using single cell RNAseq (scRNAseq).
-
Inhibitory Control of Muscle Activity in Sleep
… motor activity using both a pharmacological rat model and a genetic mouse model. I characterized the role for GABA and glycine in mediating the REM-specific suppression of muscle activity as well as their involvement in regulating the phasic muscle twitches that punctuate this atonia. Based on …
-
The Role of Wnt Signaling in Bone Mechanotransduction
… in bone during a disuse challenge, using a genetic mouse model expressing an inducible constitively-active mutant form of β- catenin in the osteocyte population. I hypothesize that prevention of β-catenin degradation during disuse will prevent the bone wasting effects of mechanodeprivation. …
-
Naᵥ1.8+ Visceral Afferent Neurons: Roles in Metabolism and Inflammation
… of this neuronal population, we generated a genetic mouse model with specific ablation of sensory vagal neurons by driving expression of diphtheria toxin (DTA) in a Cre-dependent manner through crossing the DTA mouse, which expresses DTA upon Cre activity, with the well-characterized …
-
A Dual Investigation of Questions in Drug Addiction: Exploring the Role of Neuroinflammation in Opioid Withdrawal and A Novel Measure of Reward-Seeking Behavior in Rodents
… of relapse. In the first series of experiment, a genetic mouse model lacking the key microglia immune receptor TLR4 was used to determine the involvement of this protein in the molecular and behavioral responses to opioid withdrawal, in particular those related to negative emotions. Although …
-
T Cell Immunosurveillance in Pancreatic Ductal Adenocarcinoma
… as understood from carcinogen-driven mouse models of highly mutated tumors, states that T cell-mediated immune pressure drives a continuum of tumor elimination, equilibrium, and escape. This “Triple E Hypothesis” reflects host-tumor interactions in a subset of human cancers that have …
-
A Gain-of-Function Role of Apolipoprotein E2 in Melanoma Progression
<p>Genetic polymorphism of the secreted lipid transporter apolipoprotein E (APOE) plays important roles in the development of atherosclerosis and Alzheimer's disease. More recently, three common APOE alleles have been implicated as modulators of melanoma progression and survival. Melanoma patients …
-
Recapitulation of Human Placental Insufficiency in a Novel Mouse Model :New Paradigm in Translational Research
Objective: To develop a mouse model of placental insufficiency (PI) that recapitulates important characteristics of the human PI. We tested the effects of selective reduction of placental blood flow by mesenteric uterine artery branch ligation (MUAL) reliably resulting in fetal growth restriction …
-
Brain Insulin Action Regulates Hypothalamic Glucose Sensing and the Counterregulatory Response to Hypoglycemia
… and CNS glucose uptake. Taking advantage of a genetic mouse model that chronically lacks CNS insulin action: the neuronal insulin receptor knockout "NIRKO" mouse), this report assessed whether CNS insulin signaling regulates the brain's ability to detect and respond to hypoglycemia by analyzing …
-
The Role of the Intestinal Microbiota in Inflammatory Bowel Disease
… IBD) arises from complex interactions of genetic, environmental, and microbial factors. The intestinal microbiota is crucial for IBD induction and complex shifts in microbiota composition occur in IBD, but disease has not been consistently associated with presence or absence of a specific …
-
Colonocyte-Derived Lactate Promotes Enterobacteriaceae Fitness During Non-Infectious Colitis
… colitis. We recapitulated these findings in a genetic model of colitis and found that lactate utilization via the respiratory lactate dehydrogenase LldD enhanced E. coli fitness during intestinal inflammation. We then were able to separate the contributing factors of dysbiosis and inflammation …
-
Microglia and Myelin: Improved Tools and Molecular Interactions
… the research community with much needed improved genetic tools to study the role of microglia, (2) developing analysis tools to facilitate the analysis of white-matter ultrastructure, and (3) defining the role of a candidate molecular mediator, IgG, as well as its receptor, in white-matter …
-
Obesity-Induced Alterations in Sperm Quality, Testicular Morphology, and Extracellular Vesicle Localisation in Male Mice
… male infertility. Methods: This study utilised a genetic mouse model of obesity (ob/ob) to investigate obesity-induced male infertility. Control (C57BL/6) and obese (ob/ob) male mice were euthanized at 16 weeks of age. Epididymides were harvested to extract sperm to measure sperm concentration, …
-
Interactions between BMP and Canonical Wnt Signaling Regulate Critical Stages of the Osteoblast Lifecycle
… bone "anabolic" therapies will require genetic and mechanistic information about other signaling pathways that can potently stimulate osteoblast differentiation and/or function. A wealth of genetic evidence in humans and mice clearly demonstrates that the canonical Wnt pathway is …
-
Regulation of Systemic and Cellular Metabolism In Prostate Cancer By Camkk2 and Elucidation of Atgl’S Role In Disease Progression
… we utilized the TRansgenic Adenocarcinoma Mouse Prostate (TRAMP) genetic mouse model and found that germline deletion of <em>Camkk2</em> indeed slows, but does not stop, primary prostate tumorigenesis. Interestingly, high-fat diet-fed TRAMP mice, a model of obesity-driven prostate cancer …
-
Myc and Cyclin T1 co-expression as a therapeutic strategy for cardiac repair following myocardial infarction
… the co-expression of Myc and Cyclin T1 in a genetic mouse model setting and from a bicistronic mRNA vector could drive adult rodent cardiomyocyte cell-cycle re-entry in the context of injury and lead to functional improvement. Methods Whole-heart single-nuclei RNA sequencing (snRNAseq), in …
-
PURINERGIC TRANSMISSION IN MIGRAINE: ROLE OF P2Y RECEPTORS IN THE SPINAL-TRIGEMINAL SYSTEM IN VIVO AND IN VITRO
… have been setting up both in vitro and in vivo models, as tools for evaluating the molecular and cellular role of the purinergic system in trigeminal pain transmission, in order to identify new potential targets for migraine pain therapy. In the first part of my PhD experimental work we have …
Page 1 of 2