Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"genetic interactions"”.
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The Discovery and Prediction of Genetic Interactions Using Data Science Genetic Interactions
… in order to discover as well as predict genetic interactions (GIs), i.e., functional interactions between gene pairs) so that novel functional associations between genes and their higher-order organizations (protein complexes, pathways and bioprocesses) could be established. Keeping GIs …
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Predicting Genetic Interactions Using Functional Interaction Networks
<p>Mapping genetic interactions is central to understanding cellular systems and identifying therapeutic vulnerabilities, particularly in the context of cancer. Among these interactions, synthetic lethality, where simultaneous loss of two genes is lethal but loss of either alone is tolerated, …
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Exploring genetic interactions with G-quadruplex structures
… aims, I present the first unbiased genome-wide genetic screen in cells where genes were silenced via short-hairpin RNAs (shRNAs) whilst being treated with either PDS or PhenDC3, two independent G4-stabilising small molecules. I explored gene deficiencies that enhance cell death (sensitisation) …
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Genetic interactions of repriming and translesion synthesis
… or non-B-DNA secondary structures. To probe the genetic interactions of PRIMPOL, genome-wide CRISPR/Cas9 knockout screens were undertaken in the human TK6 cell line. The most interesting group of hits were RAD18, UBE2A and REV1 - three genes with key roles in DNA damage tolerance through …
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Harnessing CRISPR-Cas9 screens to identify functional genetic interactions
The advent of CRISPR-Cas9 genetic engineering technologies has vastly improved our ability to interrogate genetic interactions in high-throughput studies. CRISPR-Cas9 screens have rapidly evolved to become the main approach for unbiased, genome-wide, forward genetic studies. This work seeks to …
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Fitness Landscapes, Genetic Interactions, and the Fitness of Hybrids
When two genetically differentiated populations or species come into contact and interbreed, their hybrid offspring will contain a mosaic of the genetic variants characterising the parental lineages, re-arranged into novel combinations. The fitness of these hybrids is central to the evolution of …
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Predicting genetic interactions in Caenorhabditis elegans using machine learning
… In particular, our main goal is the discovery of genetic interactions based on sparse and incomplete information. In our development, we use gene data from two model organisms, Caenorhabditis elegans and Saccharomyces cerevisiae. Our first method, information flow, uses circuit theory to evaluate …
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RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6
… All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with …
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The genetic interactions of PKHD1 and ATMIN in autosomal recessive polycystic kidney disease (ARPKD)
… relationships. It has been proposed that genetic modifiers may influence disease severity. Next-generation sequencing (NGS) using ChIP-Seq and RNA-Seq techniques in mouse kidneys and intermedullary collecting duct (mIMCD3) cells identified new transcriptional targets of Atmin, which did …
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Determining therapeutically actionable genetic interactions in human cancer at scale using multiplexed CRISPR screening
… screening approach to systematically identify SL interactions. A novel tRNA-based dual guide expression system was used to construct the Synergy library, enabling comprehensive and unbiased monogenic and digenic perturbation of 931 prioritised gene pairs. These pairs were identified through two …
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Exploiting Chemogenetic and Genetic Interactions In Human Cells As An Avenue For New Therapeutic Opportunities
… which enables the identification of chemogenetic interactions, or gene mutations that enhance or suppress the activity of a drug, and 2) combinatorial editing, which facilitates the examination of possible genetic interaction between the two perturbed genes. Both chemogenetic and genetic …
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Genetic Interactions of Factors That Regulate Alternative Rna Splicing In The Male Germ Line of Drosophila
… splicing of Taf1 (TBP associated factor 1). Genetic and cytological studies showed that mutants of Hfp and Taf1 both cause similar defects in meiosis and spermatogenesis. These results suggest Hfp regulates normal spermatogenesis partially through the regulation of taf1 splicing. These …
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Determination of Genetic Interactions Required for Dystrophin-Dystroglycan Function and Regulation in a Drosophila Model of Muscular Dystrophy
Im Menschen hängen genetisch diverse Formen von Muskeldystrophie (MD) mit einer Schädigung des Dystrophin-Glykoprotein Komplexes (DGC) zusammen. Der DGC besteht aus Dystrophin, Dystroglykanen, Sarkoglykanen, Sarkospan, Syntrophinen und Dystrobrevin. Die Charakterisierung des Drosophila melanogaster …
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MOLECULAR DISSECTION OF THE CROSSTALK BETWEEN ONCOGENIC AND TUMOR SUPPRESSIVE PATHWAYS: THE CASE OF TAZ-BRCA1 AND MYC-SETX GENETIC INTERACTIONS
… pathways drive tumor evolution and define genetic liabilities of cancer cells. The investigation of these interactions may reveal novel therapeutic targets that can be exploited in clinics for the selective eradication of cancer cells. With this purpose, we investigated by genetic analysis …
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Inferring context-specific essentiality networks using large-scale CRISPR-KO screens
… single-gene essentiality, but also how gene interactions shape cancer-specific essentiality landscapes. Together with collaborators, we performed a large-scale dual-knockout CRISPR-Cas9 screen to identify genetic interactions in *KRAS*-mutant colorectal cancer and triple-negative breast …
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Survival of Salmonella in the macrophage phagosome
… TnSeq, a novel genome wide approach, to identify genetic interactions with sodCI. This involved generating over 40,000 random transposon mutants in a wild type and sodC background, passaging those mutants through an intraperitoneal mouse infection model, then sequencing the input and output …
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Functional Genomic Approaches to Study Cell Polarity Regulation by G1 Cyclins in Saccharomyces cerevisiae
… G1 cyclins by systematically identifying their genetic interactions. To do this, I conducted Synthetic Genetic Array (SGA) screens using strains deleted for different combinations of cyclin genes. The results of screens with strains deleted for the G1 cyclin pairs, CLN1, CLN2, or PCL1, PCL2, …
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Notopleural Mutations Enhance Defects In Imaginal Disc Epithelial Morphogenesis And Macrochete Elongation Associated With Mutations in the Stubble-Stubbloid Locus
… and the morphogenesis of leg and wing epithelia. Genetic and cell biological analysis indicate a role for Stubble in actin cytoskeletal dynamics and cell shape changes in developing epithelia and bristles. Previously reported genetic interactions between Stubble and the Rho1 signaling pathway …
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Functional analysis of amyotrophic lateral sclerosis mutations using Drosophila cell culture models
… combines molecular biology, high-throughput genetic screening, and transcriptomics analysis to identify potential therapeutic targets of ALS. Transient Drosophila S2R+ cell models expressing wild-type or mutant forms of the ALS-associated genes FUS, SOD1, TDP43 were generated to evaluate …
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Invasion genomics, adaptive evolution, and transcriptional plasticity of Anopheles stephensi in Ethiopia and Yemen : implications for malaria transmission and control.
… control efforts. Multi-locus analyses reveal genetic interactions between An. stephensi and Plasmodium falciparum, highlighting evolutionary feedbacks within the transmission system. High-resolution whole-genome sequencing resolves population structure, connectivity, and recent demographic …
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