Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"genetic interaction"”.
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Analysis and interpretation of high-throughput chemical-genetic interaction screens.
… against genome-wide mutant arrays identifies genetic perturbations that cause sensitivity or resistance to compounds of interest. The resulting chemical-genetic interaction profiles contain information on the cellular functions perturbed by compounds and can be used to elucidate their modes of …
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Genetic interaction landscape of the Escherichia coli transcriptional factor machinery
… of unknown function. Using E. coli synthetic genetic array, a quantitative approach to scoring the fitness of single and double mutant genes, I was able to measure pairwise genetic interactions (GIs) among all TFs in rich and minimal media (RM and MM) to create a differential (DF) GI network. …
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A genetic interaction network of constitutive heterochromatin in Caenorhabditis elegans
Constitutive heterochromatin is an epigenetic compartment of animal genomes that is important for gene regulation, chromosome architecture and the maintenance of genome integrity. It is associated with the silencing of gene expression and the repression of repetitive element activity. Constitutive …
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Protein Phosphatase 2A Function in Response to Salt Stress in Arabidopsis thaliana: Defining Pathways and Genetic Interaction Networks
… rotation of root cell files. A physical interaction was detected between the A1RCN1 and C4 subunits in root tips by co-immunoprecipitation. Taken together, these results indicate that PP2A is involved in the response of Arabidopsis seedlings to sodium stress.</p> <p>Underlying mechanisms …
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Investigation of Akirin Nuclear Localization and the Molecular Interaction between Akirin and Taf4 in Drosophila
… in embryonic musculature development through its genetic interaction with the basal transcription factor, Taf4. We did this by analyzing muscle patterning in <em>akirin</em>, <em>taf4</em> double heterozygous mutants. Our data confirm a genetic interaction between <em>akirin</em> and …
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Interaction of akirin and pannier during embryogenesis of Drosophila melanogaster
… In this study, we identified and analyzed the genetic interaction of <em>akirin </em>with the transcriptional GATA factor, <em>pannier</em>. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of <em>akirin</em> …
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Predicting Genetic Interactions Using Functional Interaction Networks
<p>Mapping genetic interactions is central to understanding cellular systems and identifying therapeutic vulnerabilities, particularly in the context of cancer. Among these interactions, synthetic lethality, where simultaneous loss of two genes is lethal but loss of either alone is tolerated, …
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Incorporating diverse data to improve genetic network alignment with IsoRank
… of nodes. In addition to the original protein-interaction networks, I align genetic-interaction networks, gene-expression correlations, and chromosome localization data to improve the functional similarity of aligned genes. Alignments are evaluated with consistency measurements of protein …
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Exploiting Chemogenetic and Genetic Interactions In Human Cells As An Avenue For New Therapeutic Opportunities
… which enables the identification of chemogenetic interactions, or gene mutations that enhance or suppress the activity of a drug, and 2) combinatorial editing, which facilitates the examination of possible genetic interaction between the two perturbed genes. Both chemogenetic and genetic …
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Using 3-D Geometric Morphometric Techniques to Further Understand the Relationship Between Neanderthals and Homo sapiens
… that were replaced by Homo sapiens without any genetic interaction, which supports the taxonomical title for Neanderthals as Homo neanderthalensis, or there was at least a minimal genetic interaction between contemporaneous Neanderthals and early humans, designating Neanderthals as a subspecies …
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Using 3-D Geometric Morphometric Techniques to Further Understand the Relationship Between Neanderthals and Homo sapiens
… that were replaced by Homo sapiens without any genetic interaction, which supports the taxonomical title for Neanderthals as Homo neanderthalensis, or there was at least a minimal genetic interaction between contemporaneous Neanderthals and early humans, designating Neanderthals as a subspecies …
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Role of the immunoglobulin superfamily member Basigin in sensory neuron dendrite morphogenesis in Drosophila
… Recent studies have begun to elucidate how interactions between neurons shape dendrite morphogenesis. However, influence of the substrate upon which neurons grow their dendritic arbors in this process is relatively poorly understood. Here I have used the peripheral sensory neurons of the …
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REGULATION OF NEURAL CREST DEVELOPMENT REQUIRES FUNCTIONAL INTERACTIONS BETWEEN HDAC1, TFAP2A AND FOXD3
… development. We also uncovered a synergistic genetic interaction between tfap2a and hdac1 that is required for the specification and survival of all posterior trunk neural crest cells. We observed additive effects in cranial and trunk neural crest development in hadc1b382;tfap2alow double …
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Identifying Modulators of the Development of Acute Functional Tolerance to Ethanol in Caenorhabditis elegans.
… effects. Human studies indicate that there is a genetic component influencing disease susceptibility, and that an individual’s initial response to alcohol can predict their development of addiction. We have taken a forward genetics approach to study one component of initial response, acute …
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Lost in Translation: Variations in WNT Signaling and Other Translational Changes in a Drosophila Model of ALS
Amyotrophic lateral sclerosis (ALS) is a genetically heterogeneous neurodegenerative disease inwhich 97% of patients exhibit cytoplasmic aggregates containing the RNA binding protein TDP- 43, referred to as TDP-43 pathology. My project focused on understanding how TDP-43 pathology modifies …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
<p>Discovering synthetic lethal interactions between genes holds the key to uncovering cancer vulnerabilities, enabling the development of more effective drugs for patients. However, identifying these vulnerabilities in the complex genome of human, which comprises thousands of genes, poses a …
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The Role of Edc3 in Drosophila Melanogaster Neuronal Development
… the bulk of genes downregulated. We performed a genetic interaction NMJ screen and identified several candidate genes predicted to be targets of Edc3 for decapping and 5’-to-3’ mRNA decay to regulate neurite morphogenesis. Additionally, we found several targets that interact genetically with Edc3 …
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Machine Learning Methods for Learning Genetic Dependencies
Synthetic lethality refers to a genetic interaction where the simultaneous perturbation of gene pairs leads to cell death. Synthetically lethal gene pairs (SL pairs) provide a potential avenue for selectively targeting cancer cells based on genetic vulnerabilities. The rise of large-scale gene …
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Functional Roles of the Drosophila gypsy Insulator Components
… the exact mutation locus, indicating existing genetic interaction. Future work will be focused on the identification of mutation and find new potential molecules involved in gypsy insulator function.Work in pds5 and CP190 RNA interference in different cell lines was also documented. By far, …
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Investigation into the Akirin Subcellular Localization and Developmental Interactions
… a putative nuclear localization signal and the genetic interaction between Akirin and the Nucleosome Remodeling Deacetylase (NuRD) complex. Through transfection of <em>Drosophila</em> S2 cells with Akirin mutant constructs we were able to visualize the localization patterns of Akirin and how the …
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