Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"genetic instability"”.
-
Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability
Genetic instability in mammalian cells can occur by many different mechanisms. In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a …
-
A Study of genetic instability and DNA repair in selected mutants of streptomyces cattleya
… or derepression of a second repair system. In instability studies performed on them, the two MMCʳ mutants showed increased instability compared to the wild type at low UV dosage. However, the instability dropped to below that of the wild type at high UV dosages. Both these mutants appeared to …
-
Stability, folding and evolution of the tumour suppressor protein p16.
… which may be one of the causes of the genetic instability of p16. The roles of the protein instability and genetic instability in the process of tumourigenesis are discussed. The relationship between the stability, folding and evolution of p16 substantiated by the findings of this study …
-
Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus
… was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to …
-
Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
… their utility has been limited due to genetic instability after continued growth in cell culture. Previous studies have hypothesized this partial or complete loss of reporter gene is the result of recombination. This work describes two methods that can be used to stabilize …
-
Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
… their utility has been limited due to genetic instability after continued growth in cell culture. Previous studies have hypothesized this partial or complete loss of reporter gene is the result of recombination. This work describes two methods that can be used to stabilize …
-
Loss of Mismatch Repair in the Aging Human Hematopoietic Stem Cell
… related diseases are a result of intraorganismal genetic change, as a result of failed DNA damage repair, in the adult stem cell (ASC) population. The hematopoietic stem/progenitor cell (HPC) is a well characterized example of ASCs. In second and third chapters I show evidence of age related …
-
Diverse Roles Of Nuclear Intermediate Filaments In Proliferating Cells
… DNA damage can arrest some embryonic cells but genetic instability is a hallmark of cancer. This thesis studies the contrasting role of two nuclear intermediate filaments - Lamin A and Lamin B1 in the proliferating cells of embryonic hearts and cancer. Lamin B1 is upregulated together with …
-
Genetic Alterations in Advanced Head and Neck Cancer
Genetic alterations of the PTEN gene located on chromosome 10q23 have been found in different neoplasms. In squamous cell carcinoma of the head and neck (SCCHN) loss of heterozygosity (LOH) at 10q has been described to be associated with poor prognosis. Moreover, genetic instability of …
-
COMPREHENSIVE WHOLE GENOME SEQUENCING UNRAVELS THE COMPLEX GENOMIC LANDSCAPE OF NEUROBLASTOMA
… Small Nucleotide Variants (SNVs) and the genomic instability of NBL, a well-established marker of poor prognosis. We found unreported pathogenetic point mutations in 3 cancer-related genes, ESR1, MYH9 and SKI, the latter under-expressed in high-risk tumors. Focal and numerical copy number …
-
Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… a global, combinatorial approach to identify genetic loci that are important for a given phenotype. These two approaches complement each other in a strain improvement program. FME and IME approaches were applied to poly-3-hydroxybutyrate (PHB)production in Synechocystis PCC6803 [IME] and …
-
Identification of proteins interacting with the human mismatch repair protein MLH1
… c-MYC, a gene previously implicated in genetic instability and apoptosis. Using in vitro derived mutants of c-MYC, it has been shown that hMLH1 interacts with the leucine-zipper domain of c-MYC. The effect of elevated c-MYC expression on functional MMR was examined. An inducible c-MYC …
-
Genetic interactions of repriming and translesion synthesis
… or non-B-DNA secondary structures. To probe the genetic interactions of PRIMPOL, genome-wide CRISPR/Cas9 knockout screens were undertaken in the human TK6 cell line. The most interesting group of hits were RAD18, UBE2A and REV1 - three genes with key roles in DNA damage tolerance through …
-
Comparison of dose-dependent outcomes in induction of cytogenotoxic responses by novel glucosyl flavonoids
… Quercetin and its glucosides are known to induce genetic instability and decrease cell proliferation, which are possible mechanisms of anti-carcinogenesis in in vitro and animal studies. Using an in vitro system of Chinese hamster ovary (CHO) cells, this thesis project examined the differences in …
-
Structural and Functional Studies of ASCC1 in DNA and RNA Alkylation Damage Response
… with poor survival, Signature 3 mutations, and genetic instability markers. We determined the crystal structure of <em>Alvinella pompejana (Ap)</em> ASCC1 with 1.14 A resolution. A comparison of the ApASCC1 structure with the human (Hs) PDE domain reveals conserved features that have been …
-
Laboratory evolution and natural transformation in Acinetobacter baylyi ADP1
… use however is inhibited by the evolutionary instability of the competence, the large homology requirement for the efficient transformation of DNA into the ADP1 genome, and incomplete knowledge regarding the composition and regulation of the protein complex involved in DNA uptake. Chapters 1 …
-
IDENTIFICAZIONE DI UNA NUOVA CAUSA GENETICA IN UN CASO FAMILIARE DI ENCEFALOMIOPATIA MITOCONDRIALE E DEFICIT DI CITOCROMO C OSSIDASI.
… the Complex IV activity defect and showed genetic instability of mtDNA and mitochondrial morphological defects. The aforementioned findings shed light on novel mechanisms of mitochondrial biogenesis and mtDNA maintenance, establish the role of ERV1 homologue in the human DRS, and promote …
-
Oncogenic Pathways and Molecular Prognostics in Neuroblastoma
… bridge fromation, indicitive of elevated genetic instability. Examination of genes associated with good prognosis revealed genes involved in growth cone formation. Combination of the expressoin of growth cone associated genes with the snoRNPs resulted in a 4-gene prognostic signature. …
-
The taxonomy and ecology of the genus Thermus
… period of time, did not support the proposal of genetic instability in Thermus. Varying the pH and temperature of continuous culture did not significantly change the phenotype. Analysis of isolates from the same pools over a time course showed there to be resident and transient strains, as well …
-
Exploring the mutagenic consequences of inflammation and DNA damage
… species, which can damage DNA to promote genetic instability, another hallmark of cancer. While much research has been dedicated to studying the relationships between inflammation and cancer, it has been difficult to distinguish the relative contributions of modified cell behavior and de …
Page 1 of 2