Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"genetic encoding"”.
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Genetic encoding of a stable <i>O</i>-GlcNAc analogue
Attachment of N-acetylglucosamine to hydroxyl groups of serine and threonine residues of intracellular proteins is known as <i>O</i>-GlcNAcylation, an essential posttranslational event in mammals and most other metazoa, catalysed by a unique <i>O</i>-GlcNAc transferase (OGT) and removed by an …
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Trimethoprim-Based Chemical Tags for High Resolution Live Cell Imaging
… achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high photon-output and tailored functionalities. The trimethoprim-based chemical tag (TMP-tag) was initially developed based on the high affinity …
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Extending the utility of enzymes for site-specific targeting of fluorescent probes
Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, however, suffer from several drawbacks. Compared to chemical …
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Developing computational intelligence for helicopter flights control.
… be met rather than follow precise trajectories. Genetic algorithms (GA) generate feasible solutions to the inverse problem in which the helicopter’s trajectory is defined as a set of way-points. Two genetic encoding methods were implemented in flying a longitudinal acceleration/deceleration …
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Hydrogen Sulfide (H2S) and the Cardiovascular System
… in vitro and the consequences of deleting the genetic encoding of nitric oxide synthase on tissue H2S biosynthesis in mice. As part of this study, I show that the methods currently utilised to measure H2S are insufficiently sensitive/reliable to convincingly demonstrate the release of H2S from …
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A real-time simulation-based optimisation environment for industrial scheduling
… that combines priority dispatching rules and genetic encoding is proposed. This combination is demonstrated to be able to handle a wider range of problems or a current scheduling problem that may change over time, due to the flexibility requirements in the real-world. The novel hybrid genetic …
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Containership Load Planning with Crane Operations
… problems, a meta-heuristic approach based on genetic algorithms is designed and implemented which embeds a crane split approximation routine. The genetic encoding is ultra-compact and represents grouping, sorting and assignment strategies that might be applied to form the stowage pattern. The …
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Genetic Code Expansion in Mammalian Cells
… scientists have developed methods to expand the genetic code of living organisms, introducing new, non-canonical amino acids with diverse chemistries into proteins. These methods rely on engineering of the translational machinery often importing an aminoacyl-tRNA synthetase (aaRS)/tRNA pair that …
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Genetic Engineering of Functional Large Amyloid Fibers
"Template" and "adder" proteins can be genetically encoded to produce large amyloid fibers when mixed together. Escherichia coli is used to clone a "template" protein, Gd20, which will cooperatively self-assemble with two "adder" proteins, P7Q and P7S, to yield two different large amyloid fibers. …