Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"genetic crosses"”.
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An Insight into Alpha-Synulcien's Biological Function and Its Pathogenesis in Neurodegenerative Disease
… (A53T and A30P) in alpha -synuclein that are genetically linked to Parkinson's disease, together with alpha -synuclein being the major component in Lewy bodies, has generated extensive interest in alpha -synuclein as a key component in neurodegenerative diseases. In recent years modeling this …
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Algorithms for regulatory network inference and experiment planning in systems biology
… arises in the context of planning large-scale genetic cross experiments that can be used to validate predictions of multigenic perturbations made by mathematical models. (i) I present CrossPlan, a novel methodology for systematically planning genetic crosses to make a set of target mutants from …
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Inheritance of virulence in the root rot pathogen Phytophthora sojae
… Rps3a. To study the inheritance of virulence, genetic crosses and self-fertilizations were performed. A cross between P. sojae strains ACR10 and P7076 causes transgenerational gene silencing of Avr3a allele, and this effect is meiotically stable up to the F5 generation. However, test-crosses of …
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Characterisation of the AT4G11100 gene, a negative regulator of disease resistance in Arabidopsis thaliana
… important in the absence of pathogen attack. Genetic mapping experiments indicate that cir1 is located on the lower arm of chromosome 4 of A. thaliana and may be one of 8 known genes in the region. Analysis of T-DNA knockouts of these 8 genes suggests that AT4G11100 is the mostly likely …
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Integrin Distribution in Collagen Iv Mutants and Use of Rna Interference to Identify a Collagen Iv Receptor in C. Elegans
… ßpat-3::GFP fusion gene was mated using genetic crosses into a collagen IV (emb-9) mutant background. Generation of a stable transgenic strain was not successful, but GFP distribution in M-lines and dense bodies was analyzed on a potential crossed animal to determine integrin distribution …
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New Targets of p53 Regulation: From Aging to Immune Response
… sensor, p53. The first study describes a novel genetic model system to address the effects of p53 dosage in an in vivo context. By developing an allelic series through multiple genetic crosses, I obtained mice with incrementally increasing levels of constitutively active p53. I describe how p53 …
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BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility
… germ cell differentiation and maturation. Using genetic crosses to further impair BMP signaling, I found that zebrafish doubly heterozygous for mutations in bmpr1bb and bmp2b, bmp7a or smad5 have profoundly impaired gonadogenesis and altered male:female sex ratios. Affected fish also exhibit …
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Structure-function relationships for the small subunit (S) of ribulose-1,5-bisphosphate carboxylase/oxygenase: Foreign S expression and characterization of engineered protein
… of pea S relative to Arabidopsis S protein. Genetic crosses between independently transformed plants expressing pea S suggest that expression of different amounts of pea S can be achieved.
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Expanding Genetic and Genomic Resources for Sex Separation and Mosquito Control Strategies
… bringing together elements of classical genetics, recent advances in sequencing and genome-editing technologies, and the use of modern forward genetics approaches. Chapter 2 of this dissertation explores the use of the Oxford Nanopore Sequencing Technology for the first time in …
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Properties of the Merozygotes Associated with a Sulfonamide-Resistant Mutation in Pneumococcus
… of properties which make them amenable to genetic and physiological analysis at different levels of precision. A review of a number of such unstable systems, initially identified on the basis of a regular segregation to phenotypically non-parental types, has led to the recognition of a …
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Isolation and Identification of the Abnormal Opaque material in the White Mutant of Siamese Fighting Fish, Betta Splendens
… included to determine possible differences in genetic expression. The fish were microscopically inspected both externally and internally. Then various tissue samples were extracted (O.1N HCl) and components of the extracts separated by thin layer chromatography (1M NaCl). These separated …
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Roles of RNA interference and DNA mismatch repair in maintaining genomic integrity in Cryptococcus pathogens
… 4, experimental procedures for conducting genetic crosses with Cryptococcus, isolating meiotic products, and many factors impacting these methods are presented. The conclusions of each preceding chapter are then summarized in Chapter 5, where I also put forth further questions and …
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Investigating R gene evolution by meiotic recombination using synthetic gene clusters in Arabidopsis
… others being developed, will be used in future genetic crosses to identify and characterize plants expressing chimeric RPP1 genes. My second area of research was to use a previously developed synthetic RBCSB gene cluster (synthRBCSB) gene cluster to investigate the relative frequency of meiotic …
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Phenotypic and Genetic Diversity in the Sea Urchin Lytechinus Variegatus
… dissertation examined the geographic and genetic variability of color patterns and morphology of the sea urchin <italic>Lytechinus variegatus </italic>. This study had 3 objectives: 1) to describe and quantify phenotypic diversity--color and morphology--throughout the geographic range; 2) …
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A Forward Genetic Screen Revealed the Role of POLDIP2 in Ensuring Maternal Inheritance of Mitochondrial DNA in Drosophila melanogaster
… homeostasis and apoptosis. They house their own genetic materials known as mitochondrial DNA (mtDNA). In most animals, the inheritance of mtDNA follows a uniparental pattern, generally from the maternal side. Maternal inheritance is accomplished by mechanisms actively degrading paternal mtDNA …
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Biological, Nutritional, and Processing Factors Affecting Breast Meat Quality of Broilers
… In the first experiment, the influence of genetic strain, plane of nutrition, and age at slaughter on breast meat quality was evaluated. Breast meat from a high yield line of broilers had lower pH at 24 h postmortem (PM), greater L*, a*, and b* values, lower water holding capacity (WHC), …
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An investigation into the role of deubiquitinating enzymes in plant disease resistance
… RNAi based cosupression and the isolation of genetic crosses between ubp12 and ubp13 mutant alleles. Collectively these approaches provide the first report that AtUBP12 and AtUBP13 are functionally redundant and are required for normal plant development with homozygous ubp12 ubp13 double …
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Medaka fish as a model for complex trait genetics
… Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel — a genetic resource established by my supervisor, Prof. Ewan Birney, and collaborators — to investigate the genetic architecture of complex traits, with a particular focus on non-additive genetic effects. My research addresses two phenotypes: one …
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Mode of Action of Penicillin in Pneumococci: From Inhibition of Cellular Targets to Bacterial Death
… an extra nutritional requirement, inseparable in genetic crosses from the cid<sup>-</sup> mutation itself (i.e., growth of cid<sup>-</sup> mutants in a chemically defined medium required supplementation of the medium with a 500- 1000 kD molecular weight molecule present in yeast extract). Chapter …
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Characterization of Hessian fly from Israel
… wheat cultivars are not deployed, and of the genetic inheritance of avirulence genes (Avr) in virulent Hf. This will allow advancement in the understanding of the Hf-wheat interaction that can be used to create more effective and long-lasting control of Hf in US. Samples of a dipteran pest of …