Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"genetic construct"”.
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Resource Allocation and Process Improvement of Genetic Manufacturing Systems
… class of manufacturing systems, defined here as genetic manufacturing systems, which produce a final product with a genetic construct. Genetic manufacturing systems rely on rare molecular events for success, resulting in waste and repeated work during the deoxyribonucleic acid (DNA) fabrication …
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… so the goal of this project was to create a genetic construct consisting of upstream regulatory elements, a bile acid inducible promoter, and a ϐ- glucuronidase reporter. Cloning strategies utilized PCR to amplify desired DNA fragments and sewing methodology to combine DNA fragments. DNA …
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Mapping Genotype to Phenotype using Attribute Grammar
… and cost consuming wet lab realization of the constructs, which, as technology advances, also become in themselves more complex. By extending the concept of describing genetic expression as a language, we propose to model relations between genotype and phenotype using formal language theory. We …
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Investigating β- and γ-secretase function and processing of APP with genetic and pharmacological tools
… in real time. I also test the ability of an optogenetic nanobody to perturb tagged γS function by light-induced clustering, and outline the creation of homology directed repair templates for endogenous tagging of the catalytic subunit of γS (PSEN1), as well as BACE1, with a genetic construct …
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Construction of a COL11A1 Transgene Vector
… and perinatal lethality. Objective: 1) To construct a transgenic expression vector designed to express a human COL11A1 cDNA in a cartilage-specific manner. This transgene will be used in future studies to correct the type XI collagen defect in homozygous cho mice. 2) To place the cDNA in an …
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miR-137, schizophrenia and sleep regulation in Drosophila melanogaster.
… regions through use of the Drosophila UAS/Gal4 genetic construct. Sleep-wake behaviour was monitored and quantified using a well characterised Drosophila activity monitoring system (DAMS). Locomotor behaviour was assessed by video tracking flies and quantifying climbing ability in a negative …
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Precision fermentation for enhanced production of food ingredients, fermented foods, and chemicals
… tagatose from lactose-rich dairy byproducts were constructed by varying the copy numbers of the expression cassettes with XYL1 and GDH to change the flux of lactose metabolites and increase tagatose production. The resulting strains do not rely on plasmid-based expression which requires …