Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 41 for “"genetic circuits"”.
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Signal and noise propagation in genetic circuits
… networks and designing reliable synthetic genetic circuits. To determine how noise propagates through gene networks we measure, in single bacterial cells, expression variability and correlations between genes in a cascade and correlations with a constitutive gene. We find that noise in a …
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RNA-based portable genetic circuits and synthetic plasmids
… for the construction of portable RNA-based circuits in multiple bacteria. Secondly, we propose an RNA compensation strategy to mitigate the retroactivity generated by the interconnection of multiple modules in RNA-based circuits. The insulation strategy is computationally proven to be …
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Measuring and Analyzing Resource Competition in Genetic Circuits
… However, the design and implementation of genetic circuits is hampered by resource competition between genetic circuit parts, where separate genetic parts become coupled due to individual parts competing for the use of shared cellular resources necessary for the part's function such as …
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Loading as a design parameter for genetic circuits
… there have been several forms of synthetic genetic circuits that have been modeled and experimentally validated. Examples include the toggle switch, repressilator, various configurations of oscillators, and even logic gates. A major goal in synthetic biology is to combine these modules to …
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Modeling and controlling resource loading in bacterial genetic circuits
… create networks of interactions, referred to as genetic circuits, which endow cells with sensing, computation, and actuation capabilities. For example, bacteria can be engineered to recognize and kill cancer cells once in the bloodstream, to neutralize radioactive waste, to sense stress levels …
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Genetic circuits for functional screens of Cas12a guide RNA libraries
… describe the design and use of CRISPR-based gene circuits for screening large libraries of gRNA scaffold variants. These circuits report on the activity of scaffolds for DNA target binding and gRNA processing, a crucial function for multiplexing. The circuits employ prokaryotic transcriptional …
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Principles for composing genetic circuits in mammalian cells with a focus on miRNA sensing
… to facilitate the construction of useful genetic circuits, with a focus on circuits that sense miRNAs. miRNAs are an attractive biomarker for sensing since they regulate virtually all biological pathways in plants and animals, and because miRNA sensors can be easily designed by …
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Expanding the synthetic cell toolkit: programmable fusion for complex genetic circuits and a synthetic cell cycle
… fusion events to control and regulate complex genetic circuits originating from within synthetic cells. The second combines several synthetic cell technologies to realize a model cell cycle within these life-like liposomal bioreactors. Although the product of this dissertation is quite …
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Scaling up Genetic Circuits in Mammalian Cells: A U1-snRNA-based Platform Enables Mammalian Cells to Compute the Bitwise Inversion of the Square Root of a Number
Scaling up genetic circuits in mammalian cells can lead to a new class of therapies. As a result of my research as PhD candidate on exploring ways and engineering tools to scale up genetic circuits, I engineered and validated in HEK293FT cells a genetic circuit that allows those cells to compute …
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Biomolecular and computational frameworks for genetic circuit design
… naturally use gene regulatory networks termed "genetic circuits" to exhibit complex behaviors such as signal processing, decision-making, and spatial organization. The ability to rationally engineer genetic circuits has applications in several biotechnology areas including therapeutics, …
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A novel method for mammalian large genetic circuit assembly and delivery
Genetic manipulation of mammalian cells provides a foundation for contemporary biological research both basic and applied. Existing methods for construction and introduction of large scale exogenous genetic information into mammalian cells and for creating stable cell lines are not efficient and …
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Synthetic biology approaches for engineering diverse bacterial species
… to specific signals. In this thesis, two new genetic tools were developed to aid the construction of genetic circuits and facilitate their delivery to bacteria isolated from diverse environments. First, antisense transcription was explored as a new tool for tuning gene expression in …
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Engineering Robust and Programmable Biological Systems
… reliable, and predictable artificial gene circuits are built. In this work, we demonstrate that robust complex cellular behaviors (e.g., bistability and gene expression dynamics) can be achieved by engineering gene regulatory architecture and increasing the complexity of genetic networks. …
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Foundational platform for mammalian synthetic biology
… progress has been made in engineering novel genetic circuits in many different organisms. However, not much progress has been made toward developing a formal methodology to engineer complex genetic systems in mammalian cells. One of the most promising areas of research is the study of …
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Synthetic Auxin Engineering: Building a Biofoundry Platform
Genetic regulatory circuits control metabolism, development, and environmental response across all kingdoms of life. Genetic circuit engineering facilitates sustainable and efficient production of biopharmaceutical, chemical, fiber, and food products that keep humans healthy, nourished, and …
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Tools for RNA and cell-free synthetic biology
… of unmodified RNAs in intact cells and systems. Genetic circuits are a fundamental tool in synthetic biology; an open question is how to maximize the modularity of their design, to facilitate their integrity, scalability, and flexibility. Liposome encapsulation enables chemical reactions to …
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Bio-inspired collective analog computation
… system which efficiently models bacterial genetic circuits in a cell, which can provide a powerful modeling and simulation tool for the design and analysis of circuits in synthetic and systems biology.
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Engineering Redox-based Intercellular Communication Channels Towards the Control of Microbial Consortial Behavior
… biology has enabled facile manipulation of the genetic architectures governing these intercellular communication pathways, leading to the bottom-up assembly of designer microbial communities and the top-down studies of natural consortia pared for specific functions. This assembly process …
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Design and quantification of a tissue type specific genetic circuit in plants
Synthetic biologists aim to rationally design genetic circuits and utilize plant platforms to photosynthetically drive, self-sustainable circuits. Although plants are excellent platforms, issues and unpredictability arise from the innate complexity of multicellularity. The ability to quantitatively …
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Engineered sensors and genetic regulatory networks for control of cellular metabolism
Complex synthetic genetic programs promise unprecedented control over cellular metabolism and behavior. In this thesis, I describe the design and development of a synthetic genetic program to detect conditions underlying acetate formation in Escherichia coli. To construct this program, I first …
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