Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"gene trap"”.
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Molecular and Functional Analysis of two Gene Trap Mouse Lines
… zu erlangen ist es wichtig neue Gene, die an diesem Prozess beteiligt sind zu isolieren und zu charakterisieren. Eine hilfreiche Methode um die Genfunktion während der Entwicklung zu studieren ist die Generierung von mutierten Mauslinien, denen das voll funktionsfähige Gen fehlt. …
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The endoplasmic reticulum chaperone ERdj4 is required for survival, glucose metabolism and B cell development
… elucidate the function of ERdj4 in vivo, ERdj4 gene trap mice were generated from embryonic stem cells harboring a gene trap cassette inserted into the ERdj4 locus. ERdj4 gene trap mice expressed hypomorphic levels of ERdj4 with a 10-100 fold reduction in all tissues and cell types examined. …
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Danio rerio (Hamilton, 1822) širdies ir kraujagyslių sistemos vystymosi ir regeneracinių mechanizmų tyrimai /
… of cardiovascular development and regeneration. However, conditional mutagenesis, which is essential for dissecting a role of developmental genes in regeneration, has not been demonstrated in the adult zebrafish. This remains a main disadvantage of D. rerio model system. The main aim …
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Studying the mechanisms of cardiovascular development and regeneration in Danio rerio (Hamilton, 1822) /
… of cardiovascular development and regeneration. However, conditional mutagenesis, which is essential for dissecting a role of developmental genes in regeneration, has not been demonstrated in the adult zebrafish. This remains a main disadvantage of D. rerio model system. The main aim …
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Study of the Functional Role of ATP1A3A in the Vertebrate Nervous System
… Specifically, the α3 isoform, encoded by gene ATP1A3A in humans, has been shown to be expressed in neurons. Mutations in ATP1A3A have been linked to rapid-onset dystonia-parkinsonism and alternating hemiplegia of childhood in humans and has also been shown to cause motor deficits, neuronal …
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Funktionelle Analyse des murinen Sall4-Gens
… Funktion des SALL4 Gens der Maus anhand einer "gene trap"-Mauslinie untersucht. Dabei wurde festgestellt, daß Sall4 im Blastocysten-Stadium in der inneren Zellmasse und in Trophektodermzellen exprimiert wird. In E7.5 embryos konnte Sall4 Expression in der Mesenchymplatte nachgewiesen werden, der …
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DAPK3 Suppresses Mammary Acini Morphogenesis and is Required for Mouse Development
… of DAPK3 we</p><p>utilized the MCF10A 3D tumorigenesis model in vitro and generated a constitutive DAPK3 knockout mouse. Using the MCF10A 3D morphogenesis model we identified that loss of DAPK3 through lenti-viral mediated knockdown accelerates MCF10A acini proliferation and apoptosis while …
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Etablierung und Analyse von 'knock-out' Mausmodellen der σ1 Untereinheiten des AP 1 Komplexes
… Funktionen bekannt. In dieser Arbeit konnte ein Gene Trap Mausmodell für σ1A etabliert werden. Das heißt die Insertionsstelle des Gene Trap-Vektors konnte bestimmt werden, so dass nun eine Genotypisierung durchgeführt werden kann und bis zum Ende des Jahres werden die ersten homozygoten …
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Ubiquitin E3 ligase mediated regulation of HMG-CoA Reductase
Loss-of-function genetic screens are a powerful approach to identify the genes involved in biological processes. For nearly a century, forward genetic screens in model organisms have provided enormous insight into many cellular processes. However, the difficulty in generating and recovering …
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Identifizierung und molekulare Charakterisierung des lysosomalen Matrixproteins Serincarboxypeptidase 1
… konnte die lysosomale Lokalisation der endogenen Scpep1 bestätigt werden. Desweiteren wurde die Glykosylierung, sowie die Prozessierung des Proteins vom 55 kDa großen Präproenzym zu einer Proteinform mit einer N-terminalen 34 kDa- und einer C-terminalen 18 kDa-Untereinheit untersucht.Die …
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Generation and characterization of spred-2 knockout mice
… unclear. Therefore, Spred-2 knockout mice were generated using a gene trap approach. The Spred-2 deficiency was verified on RNA and protein levels and the lack of functional Spred-2 protein in mice caused a dwarf phenotype similar to achondroplasia, the most common form of human dwarfism. …
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WHO: A Novel Wuschel-Like Homeodomain Transcription Factor in Arabidopsis
… a novel homeodomain transcription factor gene in Arabidopsis. It shares a high degree of identity with WUSCHEL (WUS), a transcription factor involved in specifying stem cell fate in shoot and floral meristems among several plant species. This novel homeobox gene, designated as …
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Investigating non-canonical vertebral development in the zebrafish model system
… tools to label the sclerotome and used a Gal4 gene trap line to investigate sclerotome ablation. I characterised the chordacentra and chordoblasts in our model system and verified the specificity of a promoter as a chordoblast marker. With this promoter, I established a method to target the …
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Development and application of a general method for somatic cell genetics
Genetic analysis, or genetic screening, is an efficient method for identifying the set of genes involved in a biological process. In microorganisms, powerful techniques enable systematic loss-of-function screens, which have yielded critical insights into the molecular basis of many fundamental …
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Developmental Deregulation and Tumorigenesis Inhibition In 14-3-3Zeta Knockout Mouse
… of 14-3-3z in breast cancer<em> in vivo</em>, I generated a strong 14-3-3z hypomorphic mutant mouse model by Gene Trap. We found that the homozygous mutant mice are lethal neonatally due to respiratory failure. This lethality could be rescued when outbred to CD-1 or backcrossed to FVB/N mouse …
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Characterisation and functional analysis of a novel msp domain – containing protein, MOSPD1
… and function of the heart as homozygous Mospd 3 gene trap neonates displayed a right ventricle defect characterised by a thinning of the right ventricle wall. The function of Mospd 1 is not known. Whilst Mospd 3 is mammalian specific Mospd 1 is conserved in all vertebrates including Danio rerio …
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P-REX2 PH Domain Inhibition of PTEN Regulates Transformation, Insulin Signaling, and Glucose Homeostasis
… proliferation, and disrupted acini morphogenesis. Furthermore, P-REX2 cooperated with other oncogenes, including the PI3K E545K oncogenic mutant, c-MYC, and HER2 to promote proliferation, colony formation in soft agar, and tumor formation in mice. We also analyzed the effects of expression …
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The role of sphingosine kinases and SKAM1 in cutaneous wound healing
… kinases (SKs) phosphorylate sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). SKs and S1P regulate a diverse range of cellular processes, including cell proliferation, survival, differentiation, migration, smooth muscle cell contraction, inflammation, cytoskeleton …