Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"gene transfection"”.
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Modified alginates as a matrix for gene transfection in a HeLa cell model
… is one of the greatest limiting factors in gene therapy and transfection methodology. A number of synthetic vectors such as cationic polymers and liposomes have been developed for introducing 'therapeutic' genetic material into the cell. These approaches have the distinct advantage over …
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Trans-2-aminocyclohexanol as a pH-sensitive conformation switch in liposomes
… liposome has drawn much interest as drug and gene carriers that release payloads specifically at the low-pH target sites, such as in solid tumors, tissues with inflammation, and ischemia sites. Also, it helps drug/gene to escape endosome trapping and followed lysosome degradation.</p> <p>The …
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Polymeric nanocarriers with dendritic core-shell architectures
… and were also investigated towards their gene transfection capabilities. In addition, these hyperbranched polymers can be used to stabilise metal nanoparticles in water and in perfluorinated phases after appropriate functionalisation.
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IL -10 gene therapy for the treatment of pulmonary inflammation
… the potential therapeutic effect of IL-10 gene therapy for the treatment of pulmonary inflammation and to develop appropriate gene delivery and expression systems for efficient gene transfer to the lung. We evaluated various liposomal and non-liposomal agents including LipofectAMINE RTM, …
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Photo-responsive degradable poly (beta-amino ester) toward non-viral gene delivery
… (PBAE) is one of ideal candidates for non-viral gene therapy due to its desired gene delivery efficiency, biocompatibility, and biodegradability. In order to further improve the gene delivery efficiency of PBAE by facilitating the intracellular DNA dissociation, we herein report the design and …
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Responsive polymers for dynamic modulation of bio-macromolecular transport properties
… two applications: electrophoretic separation and gene transfection. Light represents an attractive trigger to change the properties of a polymer solution because it enables structural transitions to be induced under isothermal conditions without the addition of other chemical species to the …
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Optimization and toxocologic effects of cancer immuno-electrogene therapy using a tumor-targeted interleukin-12 gene construct
… electroporation (EP) and other related physical gene transfection techniques; an overview of results of electrochemogene therapy (ECGT) used to treat naturally occurring spontaneous neoplasms in dogs; and the results of comprehensive, pre-clinical toxicology testing of electrogene therapy (EGT) …
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
… to variation in retroviral populations generating mutants that exhibit altered host tropisms, resistance to antiviral drugs, and the ability to escape the host's immune system. To identify structural elements of murine leukemia virus (MLV) RT important for fidelity and drug-resistance in …
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Molecular genetics and functions of Kaposi's sarcoma-associated herpesvirus glycoproteins in viral entry and virus-induced cell fusion
… described herein, have focused on the genetic manipulation of the KSHV-BAC36 in order to address the role of K8.1 glycoprotein in virus entry and the role of the carboxyl tail a-helices of glycoprotein (gB) in virus-induced cell fusion. In addition, a panel of KSHV-glycoprotein mutants …
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Synthesis and conformational study of trans-2-aminocyclohexanol-based pH-triggered molecular switches and their application in gene delivery
… design of the pH-sensitive helper lipids for gene delivery. Protonation of TACH molecules results in a strong intramolecular hydrogen bond between the amino and its neighboring hydroxyl groups, which triggers a conformational flip, and forces changes of the relative position of other …
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Modulation of Dendritic Cells with the Interleukin-10 Gene on Polycation-Modified Polymeric Particles
Gene therapy has emerged as a field to modulate cell functions by introducing genes of interest to target cells. An emerging focus in this field is to employ non-viral vectors to deliver immunosuppressive cytokines to dendritic cells (DCs) to attenuate damaging immune responses. DCs serve as …
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Materials and biological approach to gene delivery in human embryonic stem cells
Gene delivery is an important tool used in the study and manipulation of human pluripotent stem cells for regenerative medicine purposes. However current methods of transient gene delivery are still highly inefficient. Using materials and biologically based concepts, I aim to develop new methods …
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Prominent Platforms for the Synthesis of Size-Controllable Polymeric Nanoparticles for Gene Delivery
Gene therapy, a promising and emerging medical approach, involves delivering exoge- nous DNA or RNA into host cells to manipulate their genetic expression profiles. In cancer treatment, targeting gene therapy offers significant advantages over traditional methods, such as precise control of …
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Designing a safe dendronised polymeric nanocarrier for hydrophobic drugs or gene delivery in cancer therapy
… G3(PLLA and OGPLLL) arginine dendron of third generation bearing eight peripheral hydrophobic or cationic groups (PBF and BOC or guanidine groups) were synthesised in high yield, identified and tested for purity using NMR, MS and TLC. A series of three amphiphilic system characterised by …
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Dual-Responsive Polymer and Hybrid Systems: Applications for Gene Delivery and Hydrogels
… of the thesis was to combine the ability for gene delivery of PDMAEMA with the magnetic properties of iron oxide nanoparticles to enable an activity of the gene vector in an applied external magnetic field. Another point was to apply the dual-responsive behavior of PDMAEMA (temperature and pH) …
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MRI and NMR Investigations of Transport in Soft Materials and Explorations of Electron-Nuclear Interactions for Liquid-State Dynamic Nuclear Polarization
… polymer quantity has critical impacts on in vivo gene transfection efficiency, cellular uptake, as well as toxicity of polycationic gene delivery vectors. Specifically, I investigated the complexation properties of three different polymeric "theranostic" agents, which combine an imaging …
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Investigation of Single-Cell and Blood-Brain Barrier Mechanics after Electroporation and in Primary Brain Cancers
… techniques where cell viability is desirable (gene transfection, electrofusion, electrochemotherapy) or where cell viability is undesirable (tumor ablation, cardiac ablation). Precise control of cell shape and orientation enabled by nanofiber scaffolds provides a convenient and expedient …
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Polyelectrolyte Complexes and their Therapeutic Potential
… topics of this work: First, the delivery of genes into eukaryotic cells in vitro by means of new star-shaped polycations was explored. Second, the structure of ionic multicompartment micelles (MCMs) when complexed with polyions was studied and the performance of these nanostructures as …
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Transcriptional regulation of the human alpha 2(I) procollagen gene
… regulation of the α2(1) pro collagen gene by analysing trans-acting factor interactions within the proximal promoter of the gene and to identify the genes coding for these trans-acting factors. α2(1) procollagen gene expression was examined in a number of diff erentiate<;l cell lines …
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Viral HSV1-TK gene, radiolabeled FIAU, and ganciclovir: combined gene targeted radiotherapy and suicide gene therapy for prostate cancer
The strategy of suicide gene therapy in cancer is based on the idea of enabling tumour cells, by gene transfer, to convert a non-toxic pro-drug into a toxic product. Previous work has shown that the combination of herpes simplex virus type 1 thymidine kinase gene (HSV1-tk) transfer with the …