Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"gene reporter"”.
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Imaging Myelin Basic Protein expression in a model of remyelination by Magnetic Resonance Imaging using an Organic Anion Transporter Protein gene reporter system.
… they become vulnerable to irreversible degeneration. There is, therefore, a clinical need for therapies that enhance remyelination. This can be achieved by 1) pharmacologically targeting endogenous oligodendrocyte progenitor cells (OPCs) responsible for remyelination or 2) transplantation …
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Engineering genetically-encodable MRI contrast agents for in vivo imaging
… such exogenously applied agents are complicated. Genetically-encodable MRI sensors may overcome some of these issues. In this thesis, we describe new class of MRI contrast agents which will be broadly applicable as genetically-controlled tools for in vivo imaging. The major goal of my thesis …
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Genetically encodable calcium sensors for Magnetic Resonance Imaging
… specificity to MRI, there is a need for genetically targeted, analyte-specific sensors. Calcium is a molecule of great interest to biology since its fluctuations are highly correlated with neural activity. While much progress has been made in pursuit of genetically encoded calcium sensors …
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Studies of Three Human Intestinal Opportunistic Pathogens
… opportunistic pathogens encompass many genera and include organisms such as those in the Bacteroides fragilis group (i.e. B. fragilis and B. thetaiotaomicron), Escherichia coli, and Clostridium perfringens, resulting in an array of diseases and serious health risks. Typically these …
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Analysis and characterisation of the mouse Hic2 gene
… Analysis and characterization of the mouse Hic2 gene Like Hic1 and γFBP (chicken), Hic2 cDNA coded five Krüppel-type C2H2 zinc finger domain. Through the sequencing and comparation with different protein sequences from the homolog proteins from different species (HIC1, Hic1, HIC2, γFBP, and …
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Characterization of nonsense mediated mRNA decay in Schizosaccharomyces pombe
… fission yeast Schizosaccharomyces pombe. Many genes carry introns in fission yeast and unlike in Saccharomyces cerevisiae, the genome encodes for proteins homologous to EJC components. S. pombe is a powerful model organism which is easily genetically manipulated and we envisaged that studying …
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The Prostaglandin E2 Receptor Subtype 3E and its involvement in tauopathies
… increasingly recognised as key to the pathogenesis of Alzheimer’s disease and tauopthies. Epidemiological studies report a delay in the onset of Alzheimer’s in subjects using nonsteroidal anti-inflammatory drugs (NSAIDs). NSAIDs inhibit enzymes in the cyclooxygenase-2 (COX-2) pathway which …
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Characterization of the structure and function of a <I>Bacteroides thetaiotaomicron</I> 16S rRNA promoter
… phylum. This group is as phylogenetically distinct from other Gram-negative enterics, including <I>Escherichia coli</I>, as they are from Gram-positive organisms. Furthermore, there is no cross expression between genes of <I>E. coli</I> and <I>Bacteroides</I> species. It is …
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NEU4L INDUCES ALTERATIONS ON CELL PROLIFERATION AND DIFFERENTIATION IN NEUROBLASTOMA CELL LINE, SK-N-BE
… and axin2, which have been identified as target genes of -Catenin (3), underwent a significant increase, as mRNA content, in Neu4L over-expressing SK-N-BE cells. In order to further confirm the activation of the Wnt canonical pathway, we transiently transfected the TOP-Flash luciferase vector …
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Genetic and Biochemical Studies of YqjH and YqjI: Cross-talk between Iron Homeostasis and Nickel Homeostasis in Escherichia Coli
… and nickel in bacteria by studying the yqjH-yqjI gene pair in Escherichia coli (E. coli).</p> <p>In E. coli, the yqjH gene encodes a putative ferric siderophore reductase that is also part of the Fur regulon. The yqjI gene, divergently transcribed from yqjH, encodes a novel member of the …
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Exogenous mesenchymal stromal cells in osteochondral repair
… remains no effective intervention capable of regenerating the damaged joint cartilage in OA and current clinical management for end-stage disease remains joint replacement surgery. There is therefore a need for a change in healthcare strategies to manage early disease, a shift that is likely to …
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Stepwise Reconstitution of RNA Cellular Activity
… using four different assays, including a gene-reporter cell-based assay, electrophoretic mobility shift assay, fluorescence-based binding assay, and interhelical stacking measurements using nuclear magnetic resonance imaging (NMR) to probe behavior at varying levels of reconstitution. I …
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Regulation of the beta7 integrin gene in T cells: role of the MAPK signalling pathways
… identified two TGF-β1 response regions in the β7 gene promoter termed TGFBRR-1 and TGFBRR-2 encompassing nucleotides -509 to -398 and -121 to -34. Here, TGF-β1 activation of the β7 gene proximal promoter is shown to be mediated by MAPK family members. TGF-β1 stimulation of TK-1 T cells increased …
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Generation and characterization of novel mouse models to validate the role of KLK5 protease in inflammation for pharmacological applications
… as the Netherton syndrome (NS). In NS epidermis, genetic deficiency of the LEKTI inhibitor of serine proteases results in unopposed activity of KLK5 (and other serine proteases), which promotes skin inflammation via activation of the PAR2-NF-κB-TNFα signaling axis (Briot et al., 2009). In …