Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 151 for “"gene product"”.
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The roles of human cytomegalovirus US27 gene product during virus infection
… and immune responses. HCMV encodes four genes for putative G protein-coupled receptors (GPCRs): US27, US28, UL33, and UL78. The US28 gene product is a functional chemokine receptor that exhibits a variety of signaling outcomes. In contrast, the US27 gene product is an orphan with no known …
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Functional Analysis of the Human Cytomegalovirus Ul82 Gene Product PP71 Protein During Virus Replication
… recipients, and AIDS patients. The HCMV UL82 gene encodes for the tegument protein pp71. It has previously been shown that pp71 is required for efficient virus replication at low multiplicities of infection and that it is a regulator of immediate-early gene expression. However, the mechanism …
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The Origin of Genome Instability in Cancer: Role of the Fragile Site Gene Product FHIT
… instability, defined as an elevated rate of genetic mutation at the single nucleotide and chromosome levels. Genomic instability is a facilitating hallmark of cancer in that it raises the probability of generating cancer-promoting mutations. Multiple factors contribute to the genome …
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The role of Wilms’ Tumor gene product (WT1)in CD95-mediated apoptosis in T-cell leukaemias
… the CD95L promoter and activate it. Wilms’ tumor gene 1 (WT1) encodes a four-zinc-finger protein. Alternative splicing at the pre-mRNA level yields several transcript variants, of which the best characterized are the so called WT1-KTS and WT1+KTS isoforms. The WT1-KTS isoform, which lacks three …
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The Interferon Stimulated Gene Product Lymphocyte Antigen 6 Complex, Locus E Promotes Entry of a Subset of Diverse RNA Viruses and Inhibits Infection by Coronaviruses
… immunity by inducing hundreds of IFN-stimulated genes (ISG). In a screen to identify novel antiviral factors, the Schoggins lab unexpectedly uncovered a subset of genes that enhanced viral infection. Here, I describe my personal efforts to study lymphocyte antigen 6, locus E (LY6E), a protein …
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Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene
… was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The …
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Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii
In order to elucidate the function of the nifU gene product in nitrogenase maturation in Azotobacter vinelandii. the gene product has been hyperexpressed in Escherichia coli and characterized by various biophysical techniques. Following the initial characterization, site-directed mutagenesis of …
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Identification and Characterization of the Enzymes Involved in Biosynthesis of FAD and Tetrahydromethanopterin in Methanocaldococcus jannaschii
… CO2 or other simple carbon compounds. Methanogenesis is a key process of the global carbon cycle and methanogens produce about 75-85% of all methane emissions. Besides the universally occurring coenzymes that are needed in normal metabolic pathways, such as biotin, coenzyme A, thiamine, FAD, …
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Molecular Ecology and Evolution of Fibrobacter and Its Cellulases
… their apparent phenotypic similarities, great genetic diversity was evident among strains of Fibrobacter using 165 rRNA comparative sequence analysis. Strains of Fibrobacter formed a distinct phylogenetic group. Two species, F. succinogenes and F. intestinalis have been identified at present. …
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Characterization and reconstitution of enzymes and pathways in the biosynthetic production of nitroaromatic compounds
… searches for AurF analogs, the Mangotoxin gene cluster from Pseudomonas syringae was identified as possibly making a nitroaromatic product. Isolation and identification of this cluster’s gene product was attempted based on heterologous expression of the cluster in E.coli, and fermentation …
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Studies on the structure and function of various nif and nif- associated gene products encoded within the Azotobacter vinelandii nif gene cluster
… of nitrogenase from Azotobacter vinelandii. A gene replacement strategy was developed for oligonucleotide-directed mutagenesis of these proteins and the resulting biological and biochemical effects of these changes were examined. Identification of structurally important regions in the MoFe …
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Functional analysis of plastid-encoded genes
… which most probably represent functional genes. The primary aim of this thesis was the analysis of the role of two such ORFs, designated ycfs or hypothetical chloroplast reading frames, namely ycf9 (ORF62) and ycf10 (ORF229, cemA). Both were analyzed in Nicotiana tabacum (tobacco) via …
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The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
… thesis is evidence that the S. cerevisiae SAC1 gene product could represent one aspect of the mechanism for coupling secretory pathway function and actin assembly in yeast. Mutations in SAC1 were isolated as extragenic suppressors of both Golgi and actin defects. Analysis of the SAC1 gene …
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Analysis of protein-protein interactions linked to the formation of a bacterial cytoskeleton in Mycoplasma pneumoniae
… has a cytoskeleton-like structure. Based on genetic evidence, it was proposed that the 1818 amino acids long protein HMW2 plays a central role in both formation of the cytoskeleton-like structure and adherence to its host cell (cytadherence). As gene products of the hmw2 gene (MPN310), two …
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Characterisation of trimethoprim resistance transposons and their gene products
… different plasmid/transposon mediated resistance genes. One gene, mobilised by RP4, was located on a 4 - 6 kb transposon designated Tn4135, and resulted in the mediation of transferable high level trimethoprim resistance of greater than 1000 ug/ml. Biochemical investigation of the transposon gene …
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A role for RNA localization in the human neuromuscular disease myotonic dystrophy
RNA localization, a regulated step of gene expression, is fundamentally important in development and differentiation. In multidisciplinary experiments, we discovered that RNA (mis)localization underlies the human disease myotonic dystrophy (DM). DM, the most prevalent adult muscular dystrophy, is …
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