Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"gene overexpression"”.
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Comparative Study of Colon Cancer Subclones Uncovers Potential Roles for AKAP7 and TP53RK in the Antiviral Response.
Tumour heterogeneity is a key hurdle for the effective treatment of cancer using oncolytic viruses (OVs). A better understanding of the pathways involved in delineating tumour cell resistance and hypersensitivity to OVs is critical in order to guide the development of new therapeutic strategies to …
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Optogenetic regulation of protein activity in live cell
… profile of protein activity. Conventional genetic and pharmacological approaches such as gene overexpression, use of growth factors or inhibitors have helped us delineate interaction maps of signaling components, however these techniques provide limited means to determine contribution of a …
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Analysis of the Clear Plaque Phenotype of the Bacteriophage HK75
… RNA mediated antitermination to promote early gene expression. Recent genomic analysis of HK75 and HK022 has confirmed the relatedness of these two phages and place HK75 in the lambdoid family of bacteriophages. Lambdoid phages are temperate and can adopt a lytic or lysogenic lifestyle upon …
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Gene expression in healing tendon
… While common, the underlying molecular and genetic processes of tendon damage and repair remain poorly understood. The work described herein used genome-wide expression analyses to investigate tendon injury and healing from three perspectives. The first identified novel gene expression in …
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X chromosome studies and breast and ovarian carcinoma
Skewed somatic X inactivation (XCI), X-linked gene overexpression and abnormal X content have been associated with breast and ovarian cancer. Partial or complete reactivation of the inactive X in females may be a step in breast and ovarian cancer progression, leading to overexpression of some …
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Identification and Characterization of In-planta Expressed Secreted Effector Proteins from Magnaporthe oryzae
… rice is still elusive. Previously, we employed gene expression profiling, including robust-long serial analysis of gene expression (RL-SAGE), massively parallel signature sequencing (MPSS), and sequencing by synthesis (SBS), to examine transcriptome profiles of infected rice leaves. A total of …
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Expression Pattern of Drug-Resistance Genes in Matched Clinical Isolates of Candida albicans at Variable Fluconazole Exposure Times
… clinical isolates, resistance to FLC is due to overexpression of a) the encoding genes CDR1 and CDR2 for the ABC Transporters, b) the Major Facilitator Transporter encoding gene MDR1, or c) ERG11, the azole target gene. Previous studies have shown gene expression in a matched set of clinical …
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An investigation of the mechanisms of piperaquine resistance in Plasmodium falciparum malaria
… in the plasmepsin 2 and plasmepsin 3 (PM2-PM3) genes, in parasites likely to be PPQ-resistant. In addition to assay development, this study used gene overexpression techniques and CRISPR-Cas9 gene editing to examine the functional role of molecular markers of piperaquine resistance, including …
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Recombinant Engineering Strategies for the Production of Therapeutic Coagulation Factor Proteins
… non-human mammalian cell lines and plasmid-based gene transfer techniques to engineer transgenic cell lines. This resource-intensive strategy must be carried out for each new protein of interest, a critical limitation for complex multi-protein cocktails, and can yield proteins with non-human …
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Studies Towards the Discovery of Novel Natural Products Through Functional Analysis of Environmental DNA-Encoded Type II Polyketide Synthases
… fasamycins, fasamycin A resistant mutants were generated. Next generation sequencing of these mutants, along with in vitro biochemical assays, showed that fasamycins inhibit the FabF gene of the type II fatty acid biosynthetic system. Subsequently, candidate gene overexpression studies …
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Functional Characterisation of Protein Degradation during Human Cytomegalovirus Infection
… infection. The use of HCMV deletion mutants and gene overexpression/knockdown systems identified US2 as the viral protein responsible for LMAN2L degradation in concert with the cellular E3 ligase TRC8 (translocation in renal carcinoma, chromosome 8 gene). Several potential viral interactors of …
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Elucidating mechanisms of toxicity and engineering Escherichia coli for tolerance of short chain fatty acids
… techniques for E. coli strain construction: gene knockouts, gene overexpression, directed evolution for tolerance and E. coli biocatalyst characterization techniques: specific growth rate, membrane fluidity, membrane leakage, membrane lipid analysis, hydrophobicity, carboxylic acid …
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Sigma factor engineering for natural product synthesis in Actinoplanes sp. SE50/110
… While the acarbose biosynthesis (*acb*) gene cluster and the respective pathway have been extensively studied over decades, key regulatory elements for the understanding of acarbose biosynthesis and global transcriptional regulation are less investigated. Since acarbose production is …