Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"gene manipulation"”.
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Multi-omic profiling of NPQ improved nicotiana tabacum lines under uniform light conditions
… 2016). However, the pleiotropic effects of this gene manipulation have yet to be examined. To address this a comparative, multi-omics approach, two previously described transgenic tobacco lines (VPZ23 & VPZ34) with increased rates of NPQ relaxation were analyzed. The results indicate minimal, but …
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A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation
… of transformation as an experimental method of gene manipulation. Pseudomonas putida is the natural host of TOL plasmids, ranging between 50 kbp and 300 kbp in size and encoding genes for the catabolism of toluene, meta-toluate, and xylene. These very large, single-copy plasmids are difficult to …
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Lentiviral-mediated gene delivery to investigate the functional role of neuropsychiatric genes
Genetic studies have led to the identification of several candidate genes, some novel and others established, that may contribute to the risk of developing neuropsychiatric disorders. For example, dopamine receptor genes are established candidates for a number of psychiatric disorders such as …
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Promoter analysis in transgenic sugar beet
Crop improvement by gene manipulation will often require subtle regulation of expression of foreign genes in the transgenic plants. A strategy has been defined for production of high sucrose yield ! high juice purity / low environmental impact varieties of sugar beet. This requires modification of …
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Regulation of the Target of Rapamycin Signaling Pathway in Saccharomyces cerevisiae
<p>An integrative, biochemical, genetic, and molecular biology approach utilizing gene manipulation, gene knock outs, plasmid based protein expression, and <em>in vivo</em> protein localization of fluorescence tagged proteins was employed to determine the function of an essential protein, Lst8, in …
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Nociception and the Experience of Pain Signaling Due to Nerve Damage
… in pain signal blocking. Three distinct genes have found to be associated with perceiving pain, which include Transient Receptor Potential Ankyrin 1 (TRPA1), Transient Receptor Potential Melestatin 8 (TRPM8), and Cannabinoid Receptor 1 (CNR1). We hypothesize through knockdown of TRPA1, …
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Artificial Intron Technology to Generate Conditional Knock-Out Mice
<p>Genetic engineering has been re-shaped by the invention of new tools in modern biotechnology in a way that offers precision and efficiency in modifying the genome at a single nucleotide level and/or allowing precise control of gene expression. Such gene manipulation brings about significant …
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Role of P300 Zz Domain In Chromatin Association and Histone Acetylation
… subtype of lung cancer. By CRISPR/Cas9-mediated gene manipulation, we found that p300 depletion impedes proliferation of human and mouse SCLC cells whereas CBP depletion has little or no effect. Depletion of p300 reduces the expression of critical oncogenes such as the <em>MYC</em> family genes, …
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MiR-10a Regulation of Drug Response and Cancer Stem Cell Populations in Non-Small Cell Lung Carcinomas
… activation. In NSCLC cell lines, we found that manipulation of miR-10a results in significant changes to both mRNA and protein levels of PI3K. In the context of cellular response to front-line chemotherapeutic agents used to treat NSCLC, I uncovered that miR-10a mimic decreases cell …
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Molecular investigation if the rat jejunal active nucleoside transport protein rCNT1
… nucleoside transporters belonging to a novel gene family has provided an opportunity to examine both the structure/function relationships and the tissue distributions of these proteins in detail, and thus to elucidate their physiological roles. The first of these transporters to be cloned was …
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Biochemistry and molecular biology of binding proteins for plant growth regulators.
… of the auxin binding protein (ABP) through gene manipulation have been investigated. NPA, an auxin transport inhibitor, was found to bind specifically to a crude membrane preparation from sugar beet seedling leaf cell suspension cultures. The in vitro binding was optimal at pH 4.5 and 4?C. …
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The adaptive potential of plant endopolyploidy in response to herbivory
… of apical dominance undoubtedly alters both genetic and physiological processes to maximize rapid regrowth (particularly in axillary bud break and stem elongation), recent studies have targeted possible mechanisms for facilitating rapid regrowth in overcompensating organisms. One possible …