Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"gene knockdown"”.
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Sustained hydrogel-based delivery of RNA interference nanocomplexes for gene knockdown
… treatment of diseases such as cancer, bone regeneration, muscular dystrophy and cardiovascular disease. Effective translation from bench side to clinical use of RNAi has been limited in part because upon systemic delivery the RNAi molecules are degraded by RNases and flushed by excretory …
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Predicting gene function from images of cells
… of cells. In particular, groups of related genes and their biological functions can be predicted using images from large gene-knockdown experiments. Our analysis methods focus on measuring individual cells in images from large gene-knockdown screens, using these measurements to classify …
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Characterizing Autism and Schizophrenia Using PRISM and Deep Learning
… to analyze PRISM images of neurons treated with gene knockdown treatments corresponding to genes associated with autism and schizophrenia. Similarities and differences between normal-type and disease-type synapses are identified, and underlying synaptic phenotype groups are discovered and …
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Postembryonic Polarity Modification in the Acoel Convolutriloba longifissura
… establish the bilateral body plan early in embryogenesis by patterning orthogonal body axes with polarity that is unaltered during the lifetime of most animals. While some organisms re-establish/modify body axes during regeneration and asexual reproduction, the acoel flatworm <em>Convolutriloba …
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A functional analysis of Rab11a and Rab11b in endosomal recycling
… of Rab11a and Rab11b was conducted using RNAi gene knockdown and compared with developed stable cell line expressing the wild-type and dominant negative mutants of these GTPases. We observed that Rab11a and Rab11b differentially regulate protein levels of Epidermal Growth Factor Receptor …
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Regulatory role of phosphatidylinositol 4-phosphate 5-kinases in brain inflammation
… reported that PI(4,5)P2 was important for neurodegenerative diseases. Nevertheless, expression and regulatory role of PIP5KIs in rat primary glia cell remain poorly understood. This present study has shown that PIP5KIα and PIP5KIγ are significantly upregulated in response to ganglioside mixture …
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The role of Ryanodine receptors in neuronal calcium signaling
… ions in the cytoplasm be highly regulated. Generation of Ca<sup>2+</sup> mobilizing signals in cells involves regulation by multiple components controlling Ca<sup>2+</sup> release from the internal stores, Ca<sup>2+</sup> influx across the plasma membrane, elicitation of Ca<sup>2+</sup> …
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The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
The ability to silence genes effectively at the pre-translational level through the use of short interfering ribonucleic acid (siRNA) has become a widely studied area since the discovery of the RNA interference pathway in 1998. The ability to silence genes at this point in the central dogma of cell …
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Symbionts and immune defence in lepidopteran crop pests
… pest species are constantly being dispersed and generate great concern due their resistance against chemical pesticides and Bt crops. These factors emphasize the importance to the development of technologies for rapid field diagnosis and specific environmentally-safe pest control. Here we show …
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Restoring and enhancing Argonaute2-catalyzed cleavage
… and has been harnessed as an efficient gene-knockdown tool in many eukaryotic species, but curiously, not in zebrafish. We discovered that RNAi is less effective in zebrafish at least partly because Argonaute2-catalyzed mRNA slicing is impaired. This defect can be traced to two conserved …
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Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells
… (siRNAs), are powerful tools for regulating genes, making them a promising therapy for diseases such as cancer where oncogenic genes are over-expressed. The delivery of antisense therapeutics to target cells presents a significant challenge due to the many barriers a nucleic acid must face in …
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… RNA (siRNA) exclusively to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems because they are less immunogenic and easier to chemically modify than vectors delivered by viruses. Although current …
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Innate Immune Transcription Activator Interferon Regulatory Factor-3 (IRF3) Contributes to Maladaptive Remodeling Post-myocardial Infarction
… post-MI. It has been demonstrated that targeted gene knockdown of TLR4, as well as downstream adaptor proteins and kinases, significantly improve cardiac function and overall survival. While the well-known NF-κB transcriptional factor that is downstream to TLR4 signaling has been linked to …
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Ryanodine receptors in calcium signaling pathways
… siRNA transfection methods were employed to knockdown RyR<sub>2</sub> expression levels in NG115-401L cells. We used reverse transcription and real-time PCR to evaluate RyR2 gene expression in transfected/untransfected cells. We also evaluated cytosolic Ca<sup>2+</sup> changes induced by RyR …
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Multipronged Approach to Study Glaucoma-Associated Phenotypes
… these challenges. First, we used a novel systems genetics approach to identify and validate genetic modifiers of IOP using the enlarged BXD family of strains in combination with human GWAS glaucoma cohorts. This will pave the way for improved drug development tailored to individual genotypes for …
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The role of small RNAs in caste determination and differentiation in the bumble bee, Bombus terrestris
… In this regard, little is known of the role of gene regulation by microRNAs (miRNAs), non-coding RNA molecules that regulate development in animals and plants. In this thesis I therefore investigated the role of miRNAs in caste determination and differentiation in the bumble bee Bombus …
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Investigating the role of the CCL2/CCR2 axis in monocytes and its modulation by extracellular nucleotides
… Techniques such as lentivirus-mediated gene-knockdown, RT-PCR, and HPLC complemented these studies. A pharmacological approach indicated a requirement of CCR2, Ca2+, Gαi/o-type G-proteins, PI3K, PLC, IP3Rs, RyRs, SERCA, DAGL, DAGK, and PKC for CCL2-mediated monocyte signalling and/or …
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Synthesis and Evaluation of Carbohydrate Based Cationic Polymers for siRNA Delivery and Tracking
… In the last two decades, RNA interference (RNAi) gene regulation has attracted a lot of attention for its high potency in medical applications. However, inadequate delivery of RNA molecules to intracellular target sites has become the bottleneck in both laboratory use and clinical therapy in the …
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A dynamic transcriptome technique for transcriptional profiling and gene regulatory network involving the helicase antigen (HAGE)
… in vitro proliferation studies following HAGE gene knockdown or cDNA transfection strongly indicated an association between HAGE expression and increased tumour cell proliferation. This was supported by results gained from in vivo work performed within an immuno-compromised murine model. …
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Essentiality and Regulation of Deoxyhypusination in Trypanosoma brucei
… moiety of spermidine onto the eIF5A-lysine generating deoxyhypusine which is then hydroxylated by deoxyhypusine hydroxylase to yield the final modification, hypusine. Modified eIF5A has been shown to alleviate ribosome stalling on polyproline tracts. Human and yeast encode two isoforms of …
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