Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"gene fusion"”.
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A Systems Biology Approach to Characterizing Gene Fusion Pathways in Cancer
Gene fusions have long been known to drive cancer. Initial discovery of gene fusions was opportunistic, and functional assessment was done individually and experimentally. There is no comprehensive systems biology approach to understanding the impact of gene fusions on the signaling networks within …
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Genomic Discovery of recurrent CD44-SLC1A2 Gene Fusion in Gastric Cancer
… genomic abnormalities associated with cancers, fusion genes and transcripts are particularly notable due to their cancer-specific nature and translational potential as diagnostic and therapeutic targets. While fusion genes are well-known in haematological malignancies, our knowledge of recurrent …
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Algorithms for cancer genome data analysis - Learning techniques for ITH modeling and gene fusion classification
L'abstract è presente nell'allegato / the abstract is in the attachment
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… so the goal of this project was to create a genetic construct consisting of upstream regulatory elements, a bile acid inducible promoter, and a ϐ- glucuronidase reporter. Cloning strategies utilized PCR to amplify desired DNA fragments and sewing methodology to combine DNA fragments. DNA …
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Genetic Profiling in Soft Tissue Sarcoma
Soft tissue sarcomas (STS) are a heterogeneous group of highly malignant mesenchymal tumors that account for ~1% of all malignancies. Frequent heterogeneity and pleomorphism along with suboptimal diagnostic reproducibility and insufficient prognostic markers make clinical management of these tumors …
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Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain
… fimi can be fused to heterologous proteins. The fusion proteins can be purified by affinity chromatography on cellulose or immobilized on cellulose (Ong et al., 1989b). Streptavidin, a protein produced by Streptomyces avidinii, binds to the water-soluble vitamin, D-biotin, with remarkably high …
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Mutagenesis of nifE and nifN from Azotobacter vinelandii
… amino acids for-substitution. A translational fusion between nifN and lacZ was constructed and used to study the regulation of nifEN. This gene fusion was regulated in the same manner as wild type nifN and produced a fusion protein which was enzymatically active with respect to substrates of …
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Soft Tissue Sarcoma Patterns multiplicity, heterogeneity and growth characteristics
… has studied various aspects ? pathological, genetical, and clinical ? of STS. In study I, we demonstrated that 20% of the patients in a population-based series of 818 STS developed second primary malignancies. An increased risk of developing a second malignancy was identified (SMR 1.3; 95% …
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Image analysis of prostate cancer tissue biomarkers
… of ERG (as a surrogate marker of TMPRSS2:ERG gene fusion status) and TATI (encoded by SPINK1) proteins in a large TMA of localized prostate cancer patients. We observed a mutually exclusive expression pattern, further supporting the idea of tailored treatment for genotypically different …
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Molecular investigation of the influence of environmental factors and subinhibitory antibiotic concentrations on the biofilm formation in Staphylococcus epidermidis
… production is an important step in the pathogenesis of S. epidermidis polymer-associated infections and depends on the expression of the icaADBC operon leading to the synthesis of a polysaccharide intercellular adhesin (PIA). The PIA represents a sugar polymer consisting of ß-1,6 linked …
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Purification and Characterization of Proteolytic Aspartate Transcarbamoylase (ATCase) from Burkholderia cepacia 25416 and Construction of a pyrB1 Knock-out Mutant
… and immunocompromized patients. The pyrB gene encoding ATCase was cloned and ATCase was purified by the glutathione S-transferase gene fusion system. The ATCase in B. cepacia has been previously classified as a class A enzyme by Bethell and Jones. ATCase activity gels showed that B. …
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Mapping nif genes of Rhodobacter capsulatus, and characterization of a mutant with a pleiotropic defect in nitrogen metabolism
… six linkage groups (I-VI) by transduction with gene transfer agent (GTA). Because this vector carries only 4600 bp of DNA, linkage can only be demonstrated for short chromosomal distances. In order to demonstrate linkage on a larger scale, the self- transmissible plasmid, pBLM2, which has …
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Mutagenesis and Spectroscopic Studies on Cytochrome Bd Quinol Oxidase of Escherichia Coli
Site-directed mutagenesis studies on the highly conserved residues of cytochrome bd quinol oxidase from Escherichia coli were carried out to investigate their roles in maintaining structure and function of this enzyme. Mutations on two highly conserved residues in subunit I---Glu445 and Arg391 were …
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Biosynthesis of the Nitrogenase FeMo-cofactor from Azotobacter vinelandii: Involvement of the NifEN complex, NifX and the Fe protein
… subject of one the most intensive biochemical/genetic detective cases of modern science. At the active site of nitrogenase, the FeMo-cofactor not only represents the heart of biological nitrogen fixation, but its synthesis also serves as a model for complex metallocluster biosynthesis. Research …
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Novel Oncogenic Drivers in Pediatric Gliomagenesis
… (NBS) tumors, from 118 patients using next-generation sequencing technologies. Nearly one-third of BS-HGGs, also known as diffuse intrinsic pontine gliomas (DIPGs), harbored somatic heterozygous missense mutations in <em>ACVR1</em>, coding for a receptor serine-threonine kinase involved in …
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The clinical value of genetic analyses of bone and soft tissue tumors
… is a need for ancillary techniques, such as genetic analyses, not only for diagnostic purposes, but also for obtaining information that could be useful for treatment and prognosis. Different types of bone and soft tissue tumors, both benign and malignant, were analyzed. The tumor material …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
… to engineer artificial RPA proteins through fusion of OB folds from M. acetivorans RPA I and M. thermoautotrophicus RPA. Functional studies of these engineered proteins suggested that domain fusion and module shuffling can lead to well folded polypeptides with most of the known properties of …
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Novel methods in transcriptome analysis using RNA-seq
… for transcriptome profiling based on next-generation sequencing (NGS) technologies. Using RNA-seq, we want to solve two critical challenges: identifying Splice Junctions (SJs) and annotating gene fusion transcripts. Due to the limited read length of NGS data, it is extremely challenging to …
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Measurements and analyses of transposable element activity inside living cells
… crucial roles in evolution, development, and genetic disease. However, characterization of their detailed in vivo dynamics has been limited by a lack of direct observational methods. Here, we present novel methods that quantify biophysical characteristics of TEs in unprecedented detail. For …
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