Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 140 for “"gene function"”.
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Predicting gene function from images of cells
… of cells. In particular, groups of related genes and their biological functions can be predicted using images from large gene-knockdown experiments. Our analysis methods focus on measuring individual cells in images from large gene-knockdown screens, using these measurements to classify …
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Systems Uncertainty in Systems Biology & Gene Function Prediction
… to measure the entire complement of a cells genes or gene products has led to vast stores of data which are extremely plentiful in terms of the number of items they can measure in a single sample, yet often sparse in the number of samples per experiment due to their high cost. This often …
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Reconstructing gene function and gene regulatory networks in prokaryotes
… a complex environment lies with the network of genetic interactions that process information regarding the state of the cell and its local environment. The object of this study has been to better understand bacterial genetic regulatory networks, which conceptually consist of nodes (the genes) …
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New tools for the discovery of pigment gene function
Dozens of genes contribute to the vast variation in human pigmentation. Many of these encode proteins that localize to the melanosome, the lysosome-related organelle that synthesizes pigment, but have unclear functions. Here, we describe the MelanoIP method for rapidly isolating melanosomes and …
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Characterization of the Placenta-Specific 8.1 Gene Function during Zebrafish Embryogenesis
<p>The PLAC8 gene encodes for a small, cysteine-rich protein conserved in vertebrates that is a member of a large family of PLAC8-motif containing proteins with diverse functions in animals, plants, and algae. Recently, high levels of PLAC8 expression have been detected in aggressive colorectal …
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Applications of Machine Learning in Source Attribution and Gene Function Prediction
… using whole genome sequencing data, and (2) the functional annotation of genes using single- cell RNA sequencing data. This work proposes the development of a bioinformatics pipeline tailored for identifying genomic variants, including gene presence/absence and single nu- cleotide polymorphism. …
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Mammalian-wide interspersed repeats (MIRs) and their role in mammalian gene function and evolution
… these elements can provide additional or novel function for endogenous genes. Mammalian-wide interspersed repeats (MIRs) are short interspersed nuclear elements (SINEs), belonging to the non-autonomous class of retroelements and are found in all mammals. The recruitment of an MIR element by a …
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Assessing the Role of Clusters Derived from Large Sequence Similarity Networks for Gene Function Predictions
… experiments, computational predictions of gene functions can aid in reducing a large list of candidate genes to a few promising targets. Various computational solutions have been proposed and developed for gene function prediction. These solutions utilize various forms of data, such as …
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Development and Application of Network Algorithms for Prediction of Gene Function and Response to Viral Infection and Chemicals
… methods that enables the integration of functional annotations and interaction networks across many species in order to predict the functions of genes in newly-sequenced bacteria. 2. To overcome the limitations of experimental approaches to find human proteins and processes that are …
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Hierarchical multi-label classification for protein function prediction going beyond traditional approaches
… that are in turn organized in a hierarchy. Functional classification of genes is a challenging problem in functional genomics due to several reasons. First, each gene participates in multiple biological activities. Hence, prediction models should support multi-label classification. Second, …
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Annotating and characterizing orphan gene in Zea mays via diverse RNA-Seq data
… which I refer to "dark transcriptome". Some functional genes have been identified from this dark transcriptome. Most genes in the dark transcriptome are orphan genes. Orphan genes are the recently emerged young genes, which share no sequence similarity with proteins in any other species. In …
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Genome editing with CRISPR-Cas9 in the Illinois long term selection experiment
… markedly increased our ability to characterize genes and use genetics to the benefit of agriculture. In this work, we utilize this technology to study the Illinois Long Term Selection Experiment (ILTSE), a unique germplasm resource for studies of genome evolution and genetic variants that …
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The development of gene silencing strategies for plant parasitic nematodes
… Although RNAi has been used widely to probe gene function in PRNs, this current body of work highlights a number of serious and potential issues which should be considered in future experimental designs.<br/><br/>Taken together, the data presented in this thesis: reveal that nematode …
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Studying the Molecular Mechanisms for Generating Progenitor Cells during Tail Regeneration in Ambystoma mexicanum
… the molecular mechanisms that underlie urodele regeneration. Urodele amphibians (newts and salamanders) are among the few vertebrates with the remarkable ability to regenerate lost body appendages, like the limbs and the tail. Urodele tail and limb regeneration occurs via blastemal epimorphic …
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Understanding Evolution of Gene Expression by Comparative Analysis
… adaptations can arise from changes in both gene function and gene expression. Evolution of gene function is well-documented in comparative genomics through the study of protein and DNA sequences. But the evolution of gene expression is not well-understood. The first objective of the thesis …
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Gene Inhibition Using Genetic Suppressor Elements in Escherichia Coli JM109DE3 Cells
Genetic suppressor elements (GSEs) are short biologically active gene fragments that encode inhibitory antisense RNAs or truncated proteins that function as negative dominants. GSEs can be constructed from a single gene or isolated from a multigene library consisting of short random fragments of …
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Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis
… commercially important seaweed responds at a genetic level to stresses faced in the aquaculture environment would not only be advantageous to the South African industry, but this knowledge is essential for selecting and/or engineering macroalgal strains that are either more tolerant or …
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Transposon Mutagenesis Facilitates Discovery Of Genotype-Phenotype Associations and Functional Interrogation of the <i>Mycobacterium Kansasii</i> Genome
… the ‘tubercle bacillus’. Due to a high level of genetic similarity and lower biosafety concern,<em> Mk </em>also has potential as an attractive model organism for the study of <em>Mtb</em>. Despite this, research into <em>Mk</em> gene function is limited and no genome-wide studies have been …
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Understanding Developmental Decision Making in Malaria Parasites using Single-Cell Transcriptomics
… the importance of malaria in global health, the function of ~40% of genes remains unknown. Better understanding of gene use and gene function throughout the parasite’s life cycle is needed to inform the development of much needed new drugs, vaccines, and transmission-blocking strategies. The …
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Studying lung development in vitro: expanding the genetic toolbox and analysis of SOX2 and SOX9 function
… for end-stage respiratory disease patients. Generation of mature/functional lung cells from iPSCs heavily relies on understanding of human lung development. However, analysis of lung development has largely been based on mouse models. Given the differences between mouse and human lungs in …
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