Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"gene expression signature"”.
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Self-renewal pattern-associated genes and their role in adult stem cell functions
… Fifty two self-renewal pattern associated (SRPA) genes were identified by cDNA microarray analysis with cell culture models whose self-renewal pattern could be reversibly regulated, instead of using heterogeneous ASC-enriched populations. From evaluation of whole genome transcript levels to expand …
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ZEB1 is a central mediator of the Epithelial-Mesenchymal Transition
… pathways. In this study, I sought a common gene expression signature of the EMT by comparing five mesenchymal cell lines independently derived from the same parental epithelial line using different EMT-inducing factors. The resultant EMT core signature strongly suggested a common pathway is …
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Novel Regulators of Somatic Cell Reprogramming
… to induced pluripotent stem (iPS) cells by expression of defined embryonic factors. My thesis is focused on exploring the mechanisms underlying reprogramming using a secondary mouse embryonic fibroblast model that forms iPS cells with high efficiency upon inducible expression of Oct4, Klf4, …
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Targeting integrin alpha 5 and focal adhesion kinase to overcome azacitidine resistance in higher risk myelodysplastic syndromes
… a greater enrichment of an azacitidine specific gene expression signature involving the upregulation of innate inflammatory pathways, platelet aggregation and haemostasis pathways ultimately priming HSPCs for haematopoietic differentiation. Together, my thesis supports the role of inhibiting …
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The development and validation of a novel patient-derived 3D meningioma cell culture model
… revealed an enhanced Notch1-mediated mesenchymal gene expression signature in the spheroids compared to traditional 2D monolayer cultures. These features were confirmed by the presence of other mesenchymal traits such as invasion capacity, demonstrating this spheroid model as the first meningioma …
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Expanding inducible epithelial resistance against pneumonia: novel virus, novel receptor
… spread to other organs, and produced a unique gene expression signature in epithelial cells to diminish the impact of infection. I have also pursued describing the activation cascade of RIG-I as a newly identified receptor for ODN. I have demonstrated that the RIG-I:ODN interaction is not …
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Intestinal CD4+T Cell Responses to Food
… intestinal CD4<sup>+</sup> T cells are generated in the course of eating. Finally, we show that intestinal inflammation induced by cholera toxin (CT) led to increased tissue infiltration of CD4<sup>+</sup> T cells, enriched for Th17 rather than epithelium-adapted cells. Protection …
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Machine learning-driven integration of multimodal data for deciphering breast cancer heterogeneity
… is a complex disease with a high degree of heterogeneity. The heterogeneity of BC could be detected at different biological levels using a variety of modern molecular biological techniques. These biotechniques could generate high-throughput and quantitative measurements, such as gene expression, …
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The role of mTOR signaling in Alzheimer s disease
… increases lifespan and health-span in several genetically different species. mTOR is a ubiquitously expressed protein kinase that plays a key role in regulating protein synthesis and cell growth. mTOR also is a negative regulator of autophagy induction. By simultaneously regulating protein …
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The molecular landscape of cutaneous melanoma and its clinical implications
… these cohorts and apply detailed multi-site phylogenetic analyses to uncover some of the key evolutionary changes. In the final chapter, I apply deep RNA sequencing to primary melanomas embedded within a prospective phase III clinical trial, to define and validate a gene expression signature …
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Not-so-innocent bystanders? Investigating non-antigen specific CD8+ T cell activation in autoimmune prognosis
… the Smith Laboratory identified a novel gene expression signature in T cells predictive of clinical outcome in anti-neutrophil cytoplasmic antibodies-associated vasculitis, systemic lupus erythematosus, Crohn’s disease, and ulcerative colitis that is not associated with diagnosis. Two …
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Drug repositioning and indication discovery using description logics
… consider more abstract descriptors, such as a gene expression signature, in order to characterise the potential pharmacological action of a drug (Chapter 1). On a fundamental level, repositioning opportunities exist because drugs perturb multiple biological entities, (on and off-targets) …
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Environmental stimuli shape adaptive immunity by enhancing T helper cell differentiation and the germinal centre response
… resulting in Th17 cells with a pathogenic gene expression signature. This suggests a link between ER stress-inducing conditions such as low nutrient availability, and the pathology of Th17 cell-mediated autoimmune diseases. Another physiological scenario in which the immune microenvironment …
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An innate immune response to DNA damage in prostate cancer
… men with mCRPC harbour mutations in DNA Repair genes BRCA1/ BRCA2 and ATM. This group has previously identified a molecular subtype in solid tumors characterised by STING mediated immune signalling due to abnormal DNA as a result of loss of DNA repair mechanisms. This subtype is characterised by …
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Deciphering pre-leukaemic dysregulation caused by mutant C/EBPα at the level of the single cell
… (TFs) in lineage specification, and the CEBPA gene has been identified as an early mutation in acute myeloid leukaemia (AML). N321D is a mutation affecting the leucine zipper domain of C/EBPα which has previously generated an aggressive AML phenotype in a mouse model. The first objective of my …
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Characterization of Differentiation and Prognostic Biomarkers On Cd8+ Tumor-Infiltrating Lymphocytes In Metastatic Melanoma
… receptors (KIRs), and exhibited a gene expression signature of T cell deletion. Surprisingly, ligation of BTLA by its cognate receptor, HVEM, enhanced the survival of CD8<sup>+</sup>BTLA<sup>+</sup> TIL by activating Akt/PKB. Our studies provide a comprehensive characterization of …
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Investigating CD8+ T-cell gene expression signatures as disease prognostic biomarkers in paediatric inflammatory bowel disease.
… tailored therapy. Previous investigation of gene expression profiles from CD8+ T-cells in adult IBD cohorts identified promising signatures, including a T-cell exhaustion signature, to predict disease outcome in these patients. Hypothesis and aim: We hypothesised that adult CD8+ T-cell …
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The Tumor Suppressor Notch Inhibits Head and Neck Squamous Cell Carcinoma (Hnscc) Tumor Growth and Progression By Modulating Proto-Oncogenes Axl and Ctnnal1 (Α-Catulin)
… </em>is one of the most frequently mutated genes in this cancer type. The spectrum of inactivating NOTCH1 mutations in HNSCC suggested a tumor suppressive role for this protein; however, the mechanism of its function is currently unknown.</p> <p><strong>Procedure:</strong> We used Sanger …
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The discovery and characterization of anticancer procaspase activating compounds
… expressed. Paradoxically, procaspases are generally upregulated and rarely mutated in cancers. Therefore, a small molecule that directly activates executioner procaspase-3/-7 to caspase-3/-7 to induce apoptosis in cancer cells could circumvent the defective apoptotic machinery and serve as …
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Discovery of miRNA-regulated processes in mammalian development
… seed matches in 3'UTRs suggest that over half of genes in mammalian genomes are miRNA-regulated. Many miRNAs are also ubiquitously expressed in a variety of embryonic and adult tissues. Given these observations, we have sought to address the challenge of determining which cellular and biological …
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