Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 154 for “"gene expression profiling"”.
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Gene expression profiling of myostatin null mice
… mechanisms for each effect. We examined gene expression profiles of these mice to identify downstream targets of myostatin null- and clenbuterol-induced skeletal muscle hypertrophy. Microarray analysis was not successful at identifying gene expression differences between …
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Gene expression profiling of polyamine-depleted Plasmodium falciparum
… falciparum by using differential transcriptome profiling. Suppression subtractive hybridisation generated transcripts which were potentially up-and down-regulated due to endogenous polyamine depletion within the human malaria parasite P. falciparum. The resulting transcripts were subjected to a …
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Immunohistochemical Evaluation and Gene Expression Profiling of Senile Lentigo
… elongation of rete ridges. The molecular pathogenesis underlying the initiation and formation of senile lentigines are not completely understood. The histological and immunohistochemical features that characterize facial senile lentigines and their gene expression profiles were investigated. …
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Gene expression profiling approach towards enhancement of malaria vaccine development
… priority. This dissertation describes three gene expression profiling experiments using RNA sequencing technology (RNASeq) applied to samples collected during clinical trials performed at the CAUCASECO research center in Cali, Colombia. In each case, peripheral blood samples from volunteers …
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Gene Expression Profiling of the nip Mutant in Medicago truncatula
… was analyzed in triplicate to screen for the NIP gene based on downregulated transcript levels in the mutant as compared to wild type. Further microarray data was generated from 10 days post inoculation (dpi) nip and wild type plants. Analysis of the most highly downregulated transcripts revealed …
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Cellular Immune Response And Gene Expression Profiling In Crohn's Dise
… of the cellular immune response and microarray gene expression profiling in peripheral blood and surgical tissue from cd, uc and healthy control subjects. Nested pcr detected map dna in tissue from 10/12(83%) cd patients compared to 1/6(17%) non-ibd subjects. Fluorescence in situ hybridization …
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Identifying Diagnostic Biomarkers: Gene Expression Profiling Leptin in Dental Pulp Tissue
… this study is to investigate differential leptin gene expression in hypoxic (inflammatory) conditions. DPSCs were isolated from healthy adult third molars, supplemented with 1% serum containing media or osteogenic media, and cultured in hypoxic (3%) or normoxic (21%) conditions for 21-days. Later, …
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In Vivo Gene Expression Profiling of the Plague Bacillus, Yersinia Pestis
… is valuable to better understand plague pathogenesis. Improved understanding of the mechanisms employed by Y. pestis to cause illness may lead to new vaccines or therapies. Using a long-oligonucleotide microarray to the plague bacillus I evaluated the expression profiles of in vitro and in …
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High-throughput microfluidic living cell arrays for spatiotemporal gene expression profiling
… factors, the principle regulators of gene expression. To date, experimental investigation of spatial and temporal transcription factor activation patterns has been limited by the use of destructive measurement techniques that require averaging responses over large cell populations. …
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Development and implementation of ontology-based systems for mammalian gene expression profiling
The use of ontologies in the mapping of gene expression events provides an effective and comparable method to determine the expression profile of an entire genome across a large collection of experiments derived from different expression sources. In this dissertation I describe the development of …
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Gene expression profiling in Saccharomyces cerevisiae grown at different specific gravity environments
The global gene expression profiles of industrial strains of Saccharomyces cerevisiae responding to nitrogen deficiency and very high sugar concentrations stresses were determined by oligonucleotide microarray analysis of ~ 6200 yeast open reading frames. Genomics analysis showed that 400 genes in …
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Gene expression profiling of patients with Polycythaemia Rubra Vera using cDNA microarrays
… the physiological role of the murine PRV-1 gene by whole mount in-situ hybridisation experiments did not yield reproducible results. Nevertheless, expression of the mPRV-1 mRNA could be demonstrated from embryonic day 6.5 on by RT-PCR experiments. In order to obtain a model system for …
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Molecular Classification of Basal Cell Carcinoma of Skin by Gene Expression Profiling
… clinical features, their similarity and heterogeneity at molecular levels are not fully elaborated yet. Here, by performing comparative analysis of gene expression profiling of BCC, SCC, and normal skin tissues, we could classify the BCC into three subtypes of classical, SCC-like, and …
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Gene expression profiling reveals novel actions of glucocorticoids on immune cells during inflammation
… predominantly mediates transrepression of target genes. A combination of array-based technologies and subtractive suppressive hybridisation has allowed for the identification of genes that are altered upon activation of peritoneal macrophages by LPS, and those whose expression is further modulated …
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A flow cytometry-based method for gene expression profiling of CNS cell types
In this thesis, I present a technique for profiling gene expression of specific cell types in the central nervous system (CNS), called fluorescence activated nuclei sorting (FANS). FANS utilizes flow cytometry to isolate cell nuclei from CNS subtypes and microarray analysis of nuclear mRNA. When …
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Osteocyte Activity and Skeletal Muscle Relative Gene Expression Profiling After Short-term Muscle Paralysis
… and determine if changes in gait, muscle gene expression, and markers of osteocyte activity could be detected as early as one week after Botox-induced muscle paralysis. In the present study, 20-week-old female rats were injected with botulinum toxin A (BTX, n=12) in the right hindlimb …
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The economic and clinical outcomes and policy implications of gene expression profiling in breast cancer care
… quality of life and costs in the era of genetic assays.
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Gene expression profiling in different stages of development of Arabidopsis thaliana leaftrichomes at the single cell level
… its development and differentiation changes in gene expression during life cycle of A. thaliana leaf hair cell (trichome) were explored in this work. After onset of trichome development this cell changes its cell cycle: it starts endoreduplication (a modified cell cycle in which DNA replication …
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