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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"gene expression microarray"”.
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Gene expression microarray analysis of small, purified cell subsets
Gene expression microarray technology is potentially capable of examining all of the cellular processes at the mRNA level at a given moment. One major challenge in its applicability is that most biological samples are cell mixtures and the cell type of interest is often a minor cell subset. Using …
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New approaches to open problems in gene expression microarray data
… Among the most important innovations is the microarray tecnology. It allows to quantify the expression for thousands of genes simultaneously by measurin the hybridization from a tissue of interest to probes on a small glass or plastic slide. The characteristics of these data include a fair …
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Detecting Changes in Alternative mRNA Processing From Microarray Expression Data
<p>Alternative mRNA processing can result in the generation of multiple, qualitatively different RNA transcripts from the same gene and is a powerful engine of complexity in higher organisms. Recent deep sequencing studies have indicated that essentially all human genes containing more than a …
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Post-Transcriptional Regulation by microRNAS in Pregnancy and Parturition
… and conserved mediators of contractile gene regulation in the pregnant uterus from mouse to human. Using miRNA and gene expression microarray analyses of uterine tissues, we identified a conserved family of miRNAs, the miR-200 family, that is highly induced at term in both mice and …
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A Pairwise Feature Selection Method For Gene Data Using Information Gain
… doing classification has limitations when using gene expression microarray data. For example, the robustness of top scoring pairs does not extend to some datasets involving small data size and the gene set with best discrimination power may not be involve a combination of genes. Hence, it is …
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Lung disease diagnosis from gene expression profiles
… from a broad range of lung diseases. We used gene expression microarray data from five different lung diseases—lung adenocarcinoma, lung squamous cell carcinoma, large cell lung carcinoma, chronic obstructive pulmonary disease, and asthma—as well as a non-diseased phenotype, to train a …
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Extending the Graphical Representation of four KEGG Pathways for a Better Understanding of Prostate Cancer Using Machine Learning of Graphical models
… are discussed. The work is based on Affymetrix gene expression microarray profiles and penalised Gaussian linear models. Penalisation in linear models includes choosing the most important parents and estimating the associated coefficients simultaneously using L1-regression. The sparse dataset …
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Galectin-3 Enhances The Malignant Melanoma Phenotype By Regulating Autotaxin
… patient specimens and cell lines, the over expression of galectin-3 is associated with disease progression and metastatic potential. Herein, we have sought out to determine whether galectin-3 affects the malignant melanoma phenotype by regulating downstream target genes. To that end, …
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Investigations into the effects of berry flavonoids on nutrient transport processes in Caco-2 enterocytes
… fully elucidated. To address this we carried out gene expression microarray analysis on intestinal Caco-2 cells treated with a flavonoid-rich berry extract (0.125 % w/v for 16 h). This microarray analysis identified alterations in numerous specific nutrient pathways, three of which were selected …
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Microarray-based differential gene expression of listeria monocytogenes cultures grown as biofilms and planktonic cells
Listeria monocytogenes is one of the most virulent foodborne pathogens prominent in the food industry. The correlating disease listeriosis infects 2500 individuals in the United States annually of which roughly 625 cases are fatal. Current research is emphasizing that these infections are due to …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… of this thesis was to identify TCDD-responsive genes likely to be involved in processes of toxicity. We constructed microarrays using cDNA libraries derived from zebrafish embryonic and adult heart tissue. Embryonic heart arrays were used for protocol development. The resulting workflow was …
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Nfat1 Contributes to Melanoma Tumor Growth and Metastasis By Regulating Il-8 and Mmp-3
… in melanoma progression we stably silenced NFAT1 expression in the highly metastatic cell line, A375SM, and subjected the cells to gene expression microarray analysis. We identified and validated two downstream targets of NFAT1, i.e; IL-8 and MMP-3 to be downregulated following silencing NFAT1. …
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Role of the IKKβ/NF-κB pathway in alcoholism
… acute and chronic effects of alcohol exposure. Genetic association studies in humans, gene expression microarray studies in postmortem brains of alcoholics, transcriptome meta-analysis in rodents, and drinking models in mice support the role of neuroimmune signaling in alcohol abuse disorder. …
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Unraveling the Structure and Assessing the Quality of Protein Interaction Networks with Power Graph Analysis
… from small-scale experiments focused on specific genes and proteins to genome and proteome-wide screens. One result of this endeavor is the compilation of complex networks of interacting proteins. Molecular biologists hope to understand life's complex molecular machines by studying these networks. …
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Characterisation of STRO-1 expression on human mesenchymal stem cells and identification of putative cancer stem cells in osteosarcoma: prevention by micronutrients
… human mesenchymal stem cells (hMSCs) in regenerative medicine due to their low incidence in vivo. However, their successful application is hampered by a lack of selective markers to positively identify the expanded multipotent cells. This study aimed to characterise STRO-1 antigen as a …
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The Effect of Compound L19 on Human Colorectal Cells (DLD-1)
… 94.4% purity. In the present research, specific genes involved in apoptosis induction with the treatment of the DLD-1 cells with L19 were identified. Apoptosis was measured by detecting levels of caspase 3 and 7 using the APO-one assay after treating synchronized DLD-1 cells with varying …
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A Systems-Based Approach to Examine the Molecular Basis of Interactions between Human Monocytes and Human Micro-Vascular Endothelium
… distinct subsets, defined by their relative expression of CD14 and CD16. These subsets appear to have different functional roles, which are only recently becoming delineated. Functional differences include the manner in which different monocytes interact with blood endothelial cells. …
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Examining The Role Of Angiogenic Factors And Early Developmental Defects In The Pathogenesis Of Pre-Eclampsia In A Mouse Model, Bph/5
… associated with the human condition. As the pathogenesis of PE is known to start before the symptoms begin, this disease is very difficult to study in women. As such, this mouse model is invaluable in examining early pregnancy events and elucidating the pathophysiology of this disorder. Angiogenic …
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Effect of pregnancy on adipose tissue biology in a mouse model of obesity
… has increased in parallel with that in the general population and is associated with an increase in morbidity and mortality for both mother and baby. Obese mothers are more likely to develop gestational diabetes, hypertensive disorders including preeclampsia, thromboembolic complications, …
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First survey and functional annotation of prohormone and convertase genes in the pig
… The use of sequence homology to annotate genes that code for neuropeptides in a species that have been sequenced recently, such as the pig, is not ideal because of the variability of posttranslational modification of prohormones, from which neuropeptides are derived. So far only 40 …
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