Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"gene disruption"”.
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Alternative Mrna Splicing Redefines The Landscape Of Commonly Dysregulated Genes Across The Acute Myeloid Leukemia Patient Population.
Most genes associated with acute myeloid leukemia (AML) are mutated in less than 10% of patients, suggesting that alternative mechanisms of gene disruption contribute to this disease. Here, I investigate pre-mRNA splicing events with significant variation and striking coregulation across distinct …
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Identification and preliminary characterization of the 2,5-diphenyloxazole biosynthetic pathway in streptomyces polyantibioticus SPRT
… initial efforts were made to identify the genes coding for benzoic acid synthesis and the DPO NRPS in the S. polyantibioticus SPRT genome using PCR amplification, Southern hybridization and sequencing. This led to the identification of 12 unique adenylation (A) domains (of which one was …
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Multiplexed Crispr Libraries For Cancer Functional Genomics
<p>High-throughput forward genetic screenings are invaluable tools to systematically explore genetic interactions and to link gene disruption with disease contexts. The adaptation of CRISPR/Cas9 has improved the sensitivity and specificity of functional screenings. Despite this advance, there …
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… 400 kb, 250 kb and 100 kb, respectively. Genetic screening of an AN12 transposon insertion library showed that two megaplasmids, pREA400 and pREA250, are conjugative. It is known for other bacterial systems that a relaxase encoded by the traA gene is required to initiate DNA transfer …
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The Cell Wall Integrity-Associated Map Kinase Homolog, AbSlt2 in the Necrotrophic fungus Alternaria brassicicola is Required for Pathogenicity of Brassicas
… fungus, Alternaria brassicicola, we identified a gene with high homology to the cell wall integrity-associated mitogen-activated protein (MAP) kinase, Slt2 in the yeast, Saccharomyces cerevisiae. This MAP kinase consists of a predicted 1,251-bp open reading frame, and encodes a 416-amino-acid …
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Studies on the biosynthesis of oudenone and verucopeptin
… DNA. Partial analysis and sequencing of this gene cluster revealed the presence of several open reading frames (ORFs) coding for modular polyketide synthase (PKS) type I enzymes and multifunctional, non-ribosomal peptide synthetase enzymes (NRPS). Based on the chemical structure of …
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Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins
<p>In effort to understand and isolate genes that are associated with protein secretion, the Lin-Cereghino laboratory at University of the Pacific created mutant strains of Pichia pastoris using the restriction enzyme mediated integration method. The mutants exhibited an unusual ability to …
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The receptor tyrosine phosphatase Ptp69D and the receptor tyrosine kinase Pvr in Drosophila nervous system development
… form of Ptp69D, I have identified several genes with which Ptp69D genetically interacts in photoreceptor axon targeting. Removing a single dose of the cytoplasmic tyrosine kinases Src64 or Abl or the repulsive axon guidance receptor robo enhanced the Ptp69D dominant-negative phenotype. In …
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Compartmentalization and axon guidance in the Drosophila brain
… distinct processing centers and brain morphogenesis depends on the creation and maintenance of distinct boundaries between adjacent regions to prevent cells from mixing. In the Drosophila visual system, I have found that Slit and Roundabout (Robo) proteins function to prevent cells from …
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Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis
… ring are not well understood, though the genes encoding the enzymes that carry out these cyclizations are believed to be well conserved across divergent fungal species. Here we describe in vivo gene disruption experiments in Aspergillusfumigatus that allowed us to identify candidate …
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Autophagy and EVs in cancer cells. Effect of ATG8 knockdown on the biogenesis of CD81+ extracellular vesicles.
… of both the autophagy pathway and the biogenesis of extracellular vesicles (EVs) in cells is involved in cancer development. Evidence is now emerging that these pathways may somehow be interlinked via ATG proteins. Further elucidation of the relationship between these pathways will lead to …
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Characterisation of candidate effector proteins from barley pathogen <i>Rhynchosporium commune</i>
… single nucleotide polymorphisms (SNPs) in the gene sequence of Rc2 correlate with virulence/avirulence of the R. commune strains in the barley line SLB 10-009. Finally, targeted gene disruption was used as an approach to characterise RcCDI1, Rc2 and<br/>Rsu3_07158 candidate effectors.
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Investigation of global regulators influencing styrene metabolism and bioplastic synthesis in Pseudomonas putida CA-3
The genetics and biochemistry involved in the biodegradation of styrene and the production of polyhydroxyalkanoates in Pseudomonas putida CA-3 have been well characterised to date. Knowledge of the role played by global regulators in controlling these pathways currently represents a critical …
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Development and applications of peptide barcoded nanoparticles for high-throughput screening of mRNA delivery materials in vivo
… applications in protein replacement therapy, gene editing, immunotherapy, and vaccines, owing to their versatility and precise nature. While recent years have seen dramatic improvements in the safety and efficacy of mRNA therapeutics, their functional delivery to target tissues and cells in …
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Functional Analysis of Secondary Metabolite Biosynthesis-Related Genes in Alternaria brassicicola
… from knockout mutations of each PKS and NPS gene was investigated with an emphasis on discovery of fungal virulence factors. A highly efficient gene disruption method using a short linear double stranded DNA construct with minimal elements was developed, optimized, and used to functionally …
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Effects of prolonged mitosis on neural stem cells in vivo during development
… cognitive deficits. 12 microcephaly-linked genes identified in human genetics studies encode microtubule/centrosome-associated proteins and mutations in these genes are strongly tied to disrupted mitotic processes in neural stem cells during cortical development. Yet, how perturbed neural …
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Towards functional assignment of Plasmodium membrane transport proteins: an experimental genetics study on four diverse proteins
… In this thesis, we applied experimental genetics to determine key characteristics of four selected Plasmodium MTPs, including spatio-temporal expression and phenotypical analysis of knockout mutants. We employed the murine malaria model parasite Plasmodium berghei and in vitro blood …
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Towards functional assignment of Plasmodium membrane transport proteins: an experimental genetics study on four diverse proteins
… In this thesis, we applied experimental genetics to determine key characteristics of four selected Plasmodium MTPs, including spatio-temporal expression and phenotypical analysis of knockout mutants. We employed the murine malaria model parasite Plasmodium berghei and in vitro blood …
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The Importance of RNA Sequence and Structure in the Assembly and Function of Signal Recognition Particle
… a homolog of 7SL RNA in S. pombe, showed by gene disruption that it is essential for viability, and demonstrated by mutagenesis of the gene that a highly conserved structural element in the RNA, the domain IV tetranucleotide loop, is functionally important. By substituting UUCG for the wild …
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The Role of 5' Nucleotidase in the Regulation of Morphogenesis in Dictyostelium Discoideum
… the substrate of 5NU. Dictyostelium switches its genetic program from growth to cellular differentiation when nutrients become limited. During the time course of development, the activity of 5NU is high and becomes restricted to a narrow band of cells that form the interface between the …
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