Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 168 for “"gene delivery"”.
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Bioreducible Polymer Nanosystems For Gene Delivery
… the research of bioreducible polymers for gene delivery. A series of bioreducible poly(amido amine)s (PAAs) were synthesized. They complex with DNA forming polyplex nanoparticles and layer-by-layer (LbL) thin films as gene delivery vectors. Atomic force microscope (AFM), especially in situ …
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Enhanced polymeric nanoparticles for gene delivery
The potential of gene therapy to treat disease and improve human health is tremendous. The failure of viral gene therapy clinical trials due to toxicity, immunogenicity, and carcinogenicity has been tragic and strongly motivates a non-viral approach. However, non-viral gene delivery is currently …
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
The safe and effective delivery of therapeutic genes is the most significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in …
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Novel gene delivery systems for brain targeting
… of life of patients. The possibility of using gene therapy for the treatment of these disorders is hindered due to the presence of blood-brain barrier and due to the lack of safe and efficacious gene delivery systems that can cross this barrier and express exogenous genes in global areas of the …
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Gene delivery with amphiphilic lower generation polypropylenimine dendrimer
In this work a novel lower generation amphiphilic polypropylenimine (PPI) dendrimer with good aqueous solubility was developed and tested as a gene delivery agent. PPI dendrimer generation 3 (DAB-16-Am) was substituted with a low level of cetyl chains (less than 5 molar percent) by reaction with …
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Modulatable endosomalytic, intracellularly biodegradable vectors for gene delivery
In order to achieve efficient gene delivery, we have designed p\(K_a\) modulatable oligopeptides (2COPs) by combining with lysine, histidine and cysteine residues which will bind DNA extracellularly, internalize via endocytosis, provide a tunable endosomal release mechanism, and provide a …
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Design of hybrid retroviral/synthetic gene delivery vectors
Gene therapy has the potential to revolutionize healthcare for millions of people. However, it has yet to become a common treatment for the variety of diseases that could benefit from the delivery of therapeutic genes. Limited progress is primarily due to the lack of a safe and efficient means of …
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Organosilicate nanoparticles as gene delivery vehicles for bone cells
… uptake that enhanced cell viability. A model gene delivery system was created by complexing these nanoparticles with plasmid DNA that encoded for green fluorescent protein (GFP). The effects of nanoparticle size, composition and surface charge on complex size, DNA binding affinity and …
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Quantitative analysis and characterization of intracellular gene delivery mechanisms
A goal for gene delivery research is to design vectors capable of (a) delivering transgenes to target cells, (b) yielding efficient gene expression, and (c) minimizing any immune, inflammatory, or cytotoxic response. Current research has focused on developing such vehicles using end gene expression …
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Adeno-Associated Virus-Based Gene Delivery Approaches for Tissue Engineering
… reliance on microenvironment-based strategies. Gene delivery therefore offers a valuable avenue, whereby direct modifications can be made to dynamic systems such as cell populations. The work presented in this thesis explores several strategies for improving control with the intricacies of …
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Coaxial double-walled microspheres for drug and gene delivery applications
… from CEHDA and the simulation work on the generation of compound droplets from the same process. Process conditions and solution parameters were investigated to ensure the formation of double-walled microspheres with a doxorubicin-loaded PLGA core surrounded by a relatively drug-free PDLLA …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
Tissue engineering aims to regenerate lost or damaged tissues and frequently combines therapeutic cells, biomaterial scaffolds, and therapeutic molecules in efforts to do so. One of the primary considerations in such therapies is to orchestrate the interactions between these components to regulate …
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Modifying the lung microenvironment through gene delivery of immunomodulatory cytokines
… lung microenvironment. Through the novel genetic tools l have generated, such as the inducible transgenic mouse and the gene delivery system using adeno-associated vectors, I have target delivered cytokines to the lung environment. The approach addressed challenges associated with systemic …
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Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… relationships between polymers and pDNA delivery efficacy that will result in the rational design of safe, efficient vehicles for therapeutic nucleic acid delivery. Many polymers used as DNA delivery vehicles display high cytotoxicity. Often, the polymers with the highest transfection …
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Synthesis and Characterization of Nucleobase-Containing Polyelectrolytes for Gene Delivery
… of cytotoxicity is also often observed for these gene delivery systems. The high level of cytotoxicity is attributed to high degree of cationic character for the polyplexes formed with these systems according to the proton-sponge hypothesis. One method of reducing the overall cationic character …
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Baculovirus as a gene delivery and expression vector in mammalian cells
… free radical formation and organ failure. Viral gene therapy, such as BacMam gene transfer technology (Baculovirus-based expression in mammalian cells) is an attractive option for reducing I/R injury due to its high safety profile compared to mammalian viruses. However, limiting factors, …
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Gene Delivery to the Blood-Brain Barrier for Ischemic Stroke Therapy
… approaches for the treatment of stroke. Regenerative therapies have been identified as the major need for curing stroke [1]. We explored two different carriers (poly (ethylene glycol)<sub>5k</sub>-poly (L-aspartate-diethylenetriamine)<sub>48</sub> (PEG-DET) / Pluronic P84 (P84) and exosomes …
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