Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 73 for “"gene deletion"”.
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Gene deletion and functional analysis of Fetuin-B
This study aimed at elucidating the physiological function of the serum protein fetuin-B and comparing it with its closest family member fetuin-A. Fetuin-A is known to be an inhibitor of calcium and phosphate precipitation in vivo. This has been shown in a fetuin-A-deficient animal model. In this …
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Examination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung cancer
RBM5 is a tumour suppressor gene with substantially decreased expression in most lung cancers, especially in smokers, that may result from gene deletion. The objective of this study was to determine if significantly decreased levels of RBM5 expression in the lung cancers of smokers was related to …
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Investigations Of The Effects Of Selected Y-chromosome Encoded Gene Deletion And Viral Infection On Testicular Function In A Mouse Model
… testes are affected by both Y-chromosome encoded gene loss and the direct or indirect effects of some viral infections. Primary focus into the impact of Y-chromosome gene loss and viral infection on male fertility has been primarily focused on the Y-chromosome encoded testis determining factor, …
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THE ROLE OF DOPAMINE D3 RECEPTORS IN THE CONTROL OF ALCOHOL INTAKE
… the hypothesis that dopamine D3 receptor (D3R) gene deletion or its pharmacological blockade counteracts alcohol preference and intake in a long-term voluntary ethanol intake paradigm. Methods: Mice D3R-/- and their wild type (WT) littermates, treated or not with the D3R antagonists U99194A and …
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Investigations Into a Rationally Designed Modified Live Vaccine for Equine Strangles
… exclusively. The majority of infected horses generally recover with robust immunity and are resistant to re-infection. It is therefore conceivable that the stimulation of systemic and local immunity via vaccination could assist in the control of this disease. One major obstacle in the control …
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Testing the reliability and selectivity of different bone-cell-specific Cre- expressing mouse models for studying bone cell metabolism
… DMP1-Cre transgenic mouse which has a transgene driven by DMP1 promotor that allows Cre-expression only in late stage osteoblasts and osteocytes was used. Ctsk-Cre mouse with a driven by Ctsk promoter was used so that only osteoclasts would undergo Cre-mediated recombination. E2A-Cre mouse …
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Investigating the Role of Trimeric Autotransporter Adhesins in Fusobacterium nucleatum Pathogenesis
… factors was previously limited as there was no gene deletion system available to functionally analyze F. nucleatum proteins. Interestingly, F. nucleatum has a diverse landscape of structurally unique surface adhesins called Type 5c secreted trimeric autotransporter adhesins (TAAs), which are a …
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Investigations of lipid metabolism in Yarrowia lipolytica
… mediated by the peroxisomal oxidases, or POX genes. These enzymes catalyze the formation of a trans double bond, producing the trans-2-enoyl product. Our study looked at the comparison of the Y. lipolytica prototrophic strain against a knockout of the Pox2 gene on the uptake, incorporation, …
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Molecular basis of gene-environment interactions in the pathogenesis of asthma and COPD
… (GST) are potent antioxidants. In this work, genetic polymorphisms that alter GST enzyme activity were genotyped in a family-based childhood asthma cohort (341 families, n = 1508) and analysed to investigate whether they alter the risk of developing asthma when individuals are exposed to …
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Characterization of Deoxycholate-Responsive Genes Utilized by Brucella abortus 2308 During Oral Infection
… project, RNA sequencing was used to discover genes with the greatest transcriptional changes in B. abortus subjected to deoxycholate, a host bile acid encountered by bacteria during oral infection. Gene deletion strains of B. abortus were then created and tested for susceptibility to pH and …
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Role of galanin in synaptic transmission and plasticity in the CA1 area of the rodent hippocampus
… or paired pulse facilitation ratios. Galanin gene deletion caused a significant impairment of LTP, which was only observed in basal dendrites, the magnitude ofwhich increased with age. The underlying molecular mechanism for this impairment might be a significantly faster saturation of synaptic …
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PDGFRalpha Signaling Requirements in the Development of Tissues Derived From Sclerotome and Dermatome
… Studies utilizing tissuespecific deletion permit the elucidation of cell autonomous phenotypes, while analysis of signaling point mutants permits identification of the function of specific downstream effectors. My studies use both methods to uncover the role of PDGFRalpha signaling …
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Identification and functional characterization of the mitochondrial adenine nucleotide carriers of Trypanosoma brucei
… were analyzed by sequence analyses and phylogenetic reconstruction. AACs phylogenetic reconstruction proved a strong association with yeast, funghi and plant clades, whilst separates from those AACs and from metazoans. MCP13 groups with GGCs, seems to be present only on lower eukaryotes and …
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THE ROLE OF SKELETAL MUSCLE SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) ON THE HEALTH AND MAINTENANCE OF THE MURINE MOTOR UNIT
… we used a mouse model with Cre/Lox targeted gene deletion driven by the human alpha skeletal actin promoter, resulting in experimental animals with reduced or absent muscle-derived BDNF (MuscleBDNF<sup>+/-</sup>, MuscleBDNF<sup>-/-</sup>). We examined gastrocnemius and soleus muscles across …
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Genetic Manipulation of Bacteroides for Transient Colonization of Germ-free Mice
… The ability to tease apart these dynamics by generating bacterial tools that limited bacterial exposure of germ-free mice to a certain time-period was explored in this thesis. I target genes in synthesis pathways of two uniquely-bacterial amino acids, meso-diaminopimelic acid (mDAP) and …
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Analysis Of The Mouse p100H Mutation: Implications For Two Disease Related Genes: P and Sox6
My dissertation is focused on the genetics analyses of a mouse mutation, p100H. This mutation is caused by a radiation-induced chromosomal inversion that disrupts both the p gene and Sox6. The human counterparts of these two murine genes are either known to cause human disease (human P gene) or …
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Investigation of Escherichia coli [NiFe]-Hydrogenase Maturation
… and archaea for anaerobic growth and pathogenesis. The maturation of [NiFe]-hydrogenase requires at least seven dedicated accessory proteins to assemble and insert the components of the NiFe(CN)2CO catalytic site. The penultimate maturation step is the delivery of nickel to a primed …
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A functional genomics approach: Characterisation of two Non-ribosomal peptide synthetase genes, and investigation of an adaptive response towards alkylating DNA damage in Aspergillus fumigatus.
… and subsequent characterisation of genes which may encode virulence factors, or novel drug targets. Nonribosomal peptide (NRP) synthetases have been implicated in virulence of A. fumigatus and other fungi. The work presented here reports opposing roles for two previously …
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Modelling evolution of genome size in prokaryotes in response to changes in their abiotic environment
… the classic Hutchinson niche space model, a gene was defined as a Gaussian function over a corresponding niche dimension. The cell can have more than one gene along a given dimension, and the envelope of all the corresponding responses is considered a full description of a cell’s phenotype …
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The human myeloproliferative disorders: molecular pathogenesis and clonal heterogeneity
… presence of clonal diversity in the MPD and the genetic events associated with progressive disease. Mutations in MPL were identified in 4% of ET and 7% of IMF but not in PV. Three different acquired MPL mutations were identified, one of which had been reported as an inherited allele. Although MPL …
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