Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"gene cloning"”.
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Gene cloning studies in two nocardioform bacteria
… of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of …
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Molecular Genetics in Rhodopseudomonas Sphaeroides: Transformation, Plasmids, and Gene Cloning
… of transforming Tris-treated cells. A versatile cloning vector, pUI81, was constructed from RSF1010 and a pBR322 derivative, pSL25 and shown to be effective in transforming Tris-treated R. sphaeroides.
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Gene cloning, adjustable expression and site-directed mutagenesis of Escherichia coli adenylate kinase
The gene encoding the Escherichia coli adenylate kinase has been cloned by phage Mu-mediated transposition. Subcloning of the adk$\sp{+}$ gene into pUC13 and pBR322 allowed high-level expression and a simple purification scheme for the enzyme. An optimized system was developed for the in vivo …
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USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS
… were isolated and identified using 16S rRNA gene cloning, PCR amplification and phylogenetic analysis. Detailed analysis of their halophilic and alkaliphilic physiology for adaptation in the environment was investigated using nuclear magnetic resonance spectroscopy (NMR) to detect the …
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Characterising variation in the gene for the Dichelobacter nodosus Type IV fimbrial protein: Development of a molecular typing system
… vaccines (Stewart 1989). Variation within the gene (jimA) for the fimbrial protein suQ.unit is currently the basis for the serotype classification of these bacteria. To date, no sequencing of the fimA gene has been conducted for New Zealand isolates. This study describes the development of a …
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Purification of the proteins that bind to the gp-2 promoter in Dictyostelium discoideum
… by the product of the glycogen phosphorylase-2 gene. Cloning and sequencing of the <i>gp</i>-2 gene revealed several repeated sequences in the promoter region that are putative regulatory sites. I present here the purification of a DNA binding protein that binds to the 3' "C" box sequence in the …
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Production of a soluble hydrogenase from R. Eutropha H16
… or production of molecular hydrogen (H2) and are generally classified by the structure of the catalytic site. From a biotechnology perspective, hydrogenases have been extensively studied with a view to their potential application in H2-based energy systems. Amongst a number of promising candidates …
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Identification and Characterization of In-planta Expressed Secreted Effector Proteins from Magnaporthe oryzae
… rice is still elusive. Previously, we employed gene expression profiling, including robust-long serial analysis of gene expression (RL-SAGE), massively parallel signature sequencing (MPSS), and sequencing by synthesis (SBS), to examine transcriptome profiles of infected rice leaves. A total of …
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Improvement of soybean breeding via high throughput phenotyping and disease resistance
… technology are critical to ensure the genetic improvement of crops meet future global demands for food and fuel. Field-based phenotyping platforms are being evaluated for their ability to deliver the necessary throughput for large scale experiments and to provide an accurate depiction …
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Retroviruses in the common brush-tailed possum (Trichosurus vulpecula)
… possum genomic DNA by PCR amplification using degenerate primers derived from the retroviral pol gene. Cloning, sequencing, and analysis of these PCR products revealed the presence of several families of endogenous retroviruses in the possum genome. Reverse transcriptase activity was detected in …
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Physical mapping of a 2-Mb region centered at D10S94, a locus very tightly linked to the multiple endocrine neoplasia type 2 gene(s)
… MTC, pheochromocytoma and mucosalneuromas. Genes responsible for these cancers map to the pericentromericregion of chromosome 10, as do loci responsible for dominantly inherited MTC without additional clinical features, and MEN 2A associated with askin disorder, cutaneous lichen amyloidosis. …
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Evaluation of soybean cyst nematode (SCN) resistance in perennial glycine species and genome-wide association mapping and genomic prediction study for SCN resistance in common bean and prediction of the short distance movement of soybean rust urediniospores through machine learning
… a population that overcomes all the resistance genes in soybean. The Glycine species evaluated contains many PIs that are highly resistant to SCN with 10 species classified as immune or highly resistance to three HG types, indicating a much broader resistance in these PIs. With additional work …
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Genetic improvement of aphid resistance, protein, and elemental composition in soybean
Yield Drag Associated with the Aphid Resistance Gene Rag2 from PI 200538 The soybean aphid (Aphis glycines Matsumura) is an important pest of soybean [Glycine max (L.) Merr.] that was first identified in North American during 2000. The pest can be controlled by the resistance genes Rag1 and Rag2, …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… for their respective antigens were selected for cloning and expression in plants, as this expression system is scalable, cheaper, safe and facilitates posttranslational modifications to recombinant proteins such as glycosylation. Rabbit IgG and human IgG scFvs were isolated successfully from the …
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Characterization of the soybean genome in regions surrounding two loci for resistance to soybean mosaic virus
… quantity of soybeans worldwide. Two important genes for resistance to SMV are Rsv1 and Rsv4. Alleles at the Rsv1 locus have been shown to control resistance to all but the most virulent strain of SMV. This locus has been mapped previously to the soybean F linkage group. Rsv4 is an SMV …
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Mapping of brown stem rot resistance, genetic yield gains, and yield impact of soybean cyst nematode
Fine Mapping of Resistance Genes from Five Brown Stem Rot Resistance Sources in Soybean Brown stem rot (BSR) of soybean [Glycine max (L.) Merr.] caused by Cadophora gregata (Allington & D.W. Chamb.) T.C. Harr. & McNew, can be controlled effectively with genetic host resistance. Three BSR resistance …
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PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.)
… characterization and expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.) were studied. With primers designed from conserved regions of chitinase genes from other plant species, a 710 bp fragment (CH710) containing a partial chitinase gene sequence was amplified from Kentucky …