Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “"gene activation"”.
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Gene Activation in Early Amphibian Development
… available, and easy to observe throughout embryogenesis. The control of early development and the role of the genes have also been investigated for a long time. The combination of older morphological and more recent biochemical descriptions of development under normal and experimental conditions …
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Investigating the chromatin dynamics of gene activation
… cis-regulatory elements which contribute to the activation of gene expression. The spatio-temporal control of gene expression is particularly important during embryonic development, when the expression of developmental regulators is tightly controlled. Sonic hedgehog (Shh) is an important …
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ANALYSIS OF ELECTROPHILE-INDUCED NRF2 GENE ACTIVATION
Activation of the transcription factor Nrf2 regulates expression of phase II enzymes and other adaptive responses to electrophile and oxidant stress. Nrf2 concentrations are regulated by the thiol-rich sensor protein Keap1, which is an adaptor protein for Cul3-dependent ubiquitination and …
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Non-Histone Nuclear Proteins : Patterns of Synthesis and Phosphorylation During the Cell Cycle, Redistribution at Times of Gene Activation and Properties of Subsets Interacting with HnRNA
… every cell type, the nuclear proteins are heterogeneous ; recent estimates based on two-dimensional gel electrophoresis place the total number of different HeLa cell chromosomal proteins at over 470 (Peterson and McConkey, 1976). Many of these proteins exhibit enzymatic activities associated with …
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Organ-Level Communication During Heart Regeneration In Zebrafish.
<p>Tissue regeneration has been primarily investigated as local remodeling events in response to tissue damage or loss. Recent studies, however, indicate that uninjured structures can respond to distant tissue trauma and, in some cases, regulate tissue regeneration. One of the key questions that …
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The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
… regulated similar sets of pluripotency genes in mouse embryonic fibroblasts (MEFs). Pioneer factor mFoxA1 could not up-regulate most pluripotency genes and their binding targets of neurogenic genes in MEFs while pioneer factors are proposed to bind to their targets even if they may …
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Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer
… CDK9 is also essential for maintaining gene silencing at heterochromatic loci. Through a live cell screen, we discovered that CDK9 inhibition reactivates epigenetically silenced genes in cancer, leading to restored tumor suppressor gene expression and cell differentiation, along with …
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The Role of DNA Binding in Transcription Activation by Estrogen Receptor
… us with a useful tool to study the mechanisms of gene activation by estrogen. XER/2AH exhibited 2-3 fold increased hormone-dependent transcription. Using a minimal promoter containing two copies of the estrogen response element (ERE) linked to a TATA box, it exhibited a 10 fold increase in …
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Regulation of SPARC Gene Expression by the Activator Protein 1 Transcription Factor
<p>Overexpression of the c-Jun proto-oncogene in MCF7 breast cancer cells results in a variety of phenotypic changes related to malignant progression including a shift to estrogen independent growth, increased cell motility and invasion. Concurrent with these phenotypic changes are alterations to …
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ROLE OF A SPECIFIC NANOG SUPER-ENHANCER ERNA IN EPIGENETIC REMODELING AND ENHANCER-PROMOTER INTERACTION
… role these transcripts carry out concerning gene regulation. In this work, the role of eRNAs transcribed from the region 5kb upper the TSS of Nanog gene in mESCs has been investigated. In particular, a specific eRNA transcript, here defined as antisense eRNA, resulted actively involved in the …
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Trim24-Regulated Estrogen Response Is Dependent On Specific Histone Modifications In Breast Cancer Cells
… <p>of ERα-mediated transcriptional activation is dependent on specific histone</p> <p>modifications in tumorigenic human breast cancer-derived MCF7 cells. In the first</p> <p>part, I proved that TRIM24-PHD finger domain, which recognizes unmethylated</p> <p>histone H3 lysine K4 …
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The Role of EZH2 in the Induction and Maintenance of Acute Myeloid Leukaemia
Epigenetic regulators are commonly mutated in cancer. Activating mutations and overexpression of EZH2 occur in lymphoma and other malignancies, while loss-of-function mutations are found in myeloid malignancies. This thesis is a study of this apparent contradiction, examining the importance of …
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Modeling and controlling resource loading in bacterial genetic circuits
… Through the addition of synthetic genes, biomolecules are produced that create networks of interactions, referred to as genetic circuits, which endow cells with sensing, computation, and actuation capabilities. For example, bacteria can be engineered to recognize and kill cancer …
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Stochastic Activation of Enhancers in the Innate Immune Response by the Histone Demethylase JMJD2D
… involving DNA. In particular, transcriptional activation depends on the interplay of dozens of chromatin modifiers to establish an epigenetic landscape permissive of gene transcription. Among the most dynamic histone modifications are the acetylation and methylation at histone 3 lysine 9, but …
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Signal specificity amongst STAT1- and STAT3- inducing cytokines in the context of Th17 differentiation
… of these three chapters, we propose a simple genetic network which is capable of translating IL-6's high amplitude, transient STAT3 signal into a pro-inflammatory response and IL-10's low amplitude, sustained STAT3 signal into an antiinflammatory response. This network is able to predict that …
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Cloning and Estrogen Regulation of the Retinol Binding Protein Messenger-Rna of Xenopus Laevis
… by which the hormone, estrogen, regulates gene expression in the frog, Xenopus laevis. In this thesis, I described the isolation of a full length cDNA clone for an estrogen induced mRNA isolated from the liver of Xenopus laevis. By DNA sequencing and subsequent homology alignment with the …
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The role of the SWI/SNF ATP dependent chromatin remodelling complex in the regulation of the human hair follicle cell proliferation and control of the human cutaneous wound healing
Epigenetic regulation of gene expression occurs at a number of levels including covalent DNA and histone modifications, nucleosome positioning and ATP-dependent chromatin remodelling as well as higher order chromatin folding and 3D genome organisation. ATP-dependent chromatin remodelling complexes …
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Methods for CRISPR Cas12a Multiplexing in Mammalian Systems
… these proteins have been adapted for a number of gene editing applications. The fusion of additional proteins of interest to dead nuclease (non-cutting) mutants of CRISPR proteins such as Cas9 and Cas12a have allowed for a wide range of gene editing activities, including gene activation (CRISPRa). …
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Identifying Functional Enhancers For Fibrotic Gene Regulation In Liver Fibrosis
<p>Liver fibrosis is characterized by progressive activation of proliferating and migrating myofibroblasts that lead to accumulation of extracellular matrix (ECM). These myofibroblasts most arise from activated liver-resident hepatic stellate cells (HSCs). There is an increasing number of patients …
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