Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"gel shift assay"”.
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
… is required for efficient integration. Using the gel shift assay technique, we have shown that IntDOT binds attDOT weakly and binding of IntDOT is enhanced in the presence of IHF. We propose that IHF bends attDOT DNA during recombination, facilitating the binding of CTnDOT integrase to attDOT. …
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Modulatable endosomalytic, intracellularly biodegradable vectors for gene delivery
… are ~100 nm, and are positively charged. Gel shift assay revealed that RcPCs have less potential than RPCs to be used as vectors as they are less stable extracellularly than the RPCs. In addition, chloroquine was required to enhance the transfection of RcPCs. Furthermore, there is no …
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Validation-based insertional mutagenesis (VBIM) technology identifies adenomatous polypossis coli (APC) like protein (ALP) as a novel negative regulator of NF-κB
… led to decreased NF-κB activity by κB reporter assay and electrophoresis mobility gel shift assay (EMSA). The current project aims to further evaluate the role of ALP in the regulation of NF-κB signaling in CRC cells. We found that overexpression of ALP in human CRC HT29 cells greatly reduced …
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Developing potential drugs for the treatment of myotonic dystrophy: from rational design to lead development
… are elucidated using a combination of synthesis, gel shift assay, and molecular dynamics simulation. During the course of study, a lead compound for the type 2 myotonic dystrophy (DM2) was discovered. The synthesis, biophysical studies, and in vitro activity of this compound is described in …
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Development And Study Of Inhibitors Of Heat Shock Protein 70 Induction
… footprinting. These polyamides were evaluated by gel shift assay for their ability to block binding of the heat shock factor and the most effective polyamide was shown to decrease HSP70 expression in Jurkat cells by western blot assay.
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… The polyclonal antibodies were also used in the gel-shift assay to show the AHR dependent gel shift. Cellular delivery CΔ553 was achieved using the protein transduction domain from the HIV-1 virus transactivating protein (TAT). In order to deliver the CΔ553 into mammalian cells, an …
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New methodologies for studying the biological activity of platinum complexes
… some of which were recognized by HMG1 in a gel shift assay. In order to identify the platinum complex(es) responsible for this behavior, a sublibrary synthesis and screening approach was employed. After three iterations of sublibrary synthesis and screening, [Pt(lysine)C12] (Kplatin) was …
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Prostate Specific Membrane Antigen (PSMA): Immunoassay Development and Characterization of Transcriptional Regulation
… study is to develop a clinically useful immunoassay for quantitation of serum PSMA and to study the molecular mechanism underlying the upregulation of the PSMA gene after androgen deprivation therapy. Surface Enhanced Laser Ionization/Desorption (SELDI) ProteinChip® mass spectrometry was used …
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The identification and characterisation of proteins interacting with SUMO-ubiquitin hybrid chains
… in an in vivo context. A proximity ligation assay was developed to probe the association between SUMO, ubiquitin and RAP80, suggesting a tight association between the three after DNA damage inducing stimuli. Interesting, the SUMO targeted ubiquitin E3 ligase RNF4 was shown not to be required …
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Human Gonadotropin-Releasing Hormone (hGnRH) Gene Expression and Hormone Regulation in Human Placental JEG-3 Cells
… confirmed these observations. Further, supershift assay using Oct-1 antibody demonstrated the involvement of Oct-1 in the FP4 DNA-protein interaction in JEG-3 cells.</p> <p>Transient transfection studies showed that hERα and hERβ mediate inhibitory effects of estradiol on the hGnRH upstream …
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Determination of the Binding Site and the Key Amino Acids on Maize β-Glucosidase Isozyme Glu1 Involved in Binding to β-Glucosidase Aggregating Factor (BGAF)
… mutants K81E and T82Y failed to bind BGAF in the gel-shift assay and their frontal affinity chromatography (FAC) profiles were essentially similar to that of sorghum β-glucosidase (dhurrinase 2, Dhr2), a non-binder, indicating that these two amino acids within Ile⁷²-Thr⁸² region are essential for …
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Discovering the Potential of Photoluminescent Ruthenium(II) Complexes as Photodynamic Therapy Agents
… couples attributed to dpp0/-1 at 0.98 V. DNA gel shift assays demonstrate that complexes 1 and 2 modify DNA in the presence and absence of 3 O2 upon light activation to convert supercoiled DNA to a mixture of open circular (OC) DNA and a species that exhibit sa distinctly different migration …
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The Design, Synthesis and Study of Mixed-Metal Ru,Rh and Os, Rh Complexes with Biologically Relevant Reactivity
… as trimetallics, exhibit a more intense blue-shifted emission with a longer lifetime, which is from the same 3MLCT excited state. At 77 K, the low temperature emission from the same 3MLCT state of [{(phen)2Ru(dpp)}2RhCl2]5+ blue-shifts to 706 nm with the emission lifetime of 1.8 ms and the …