Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"gel retardation"”.
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Characterization of the Components of Carbon Catabolite Repression in Clostridium perfringens
… could not take place. We have also shown that in gel retardation assays, CcpA and HPr-Ser-P were able to bind to two DNA fragments containing putative C. perfringens CRE-sites, sequences where CcpA binds to regulate transcription. The genome sequence of a food poisoning strain of C. perfringens …
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Control of major histocompatibility Class I antigen expression in adenovirus transformed cells.
… assays and in the case of CRE1, also by gel retardation and Western blotting. The transient expression assays did not demonstrate a functional role for these sequences in transcriptional down-regulation. The implications of this result in relation to the experimental design are discussed. …
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Untersuchungen zur Regulation des Insl3 Gens
… der Promotorregion des Insl3 Gens sollte durch Gel Retardation Assays ein cis-ständiges Element charakterisiert und der daran bindende transagierende Faktor als Mitglied der hsp110 verwandten Heatshock-Proteinfamilie identifiziert werden. Durch Luciferaseassays sollte die Beteiligung des …
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… Polymer-siRNA binding strength was analyzed by a gel retardation assay. NP size and zeta potential were measured using NanoSight and zetasizer instruments, respectively. siRNA transfection efficiency was assessed by the amount of green fluorescent protein detected in GBM cells using a fluorescence …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… upstream of the ATG initiation codon. Gel retardation assays demonstrated that there were at least two nuclear DNA binding proteins from vegetative amoebae (V 1 and V2 factors) and two from developing cells (D 1 and D2 factors). Experiments with a luciferase reporter gene suggested that …
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The regulatory roles of PyrR and Crc in pyrimidine metabolism in Pseudomonas aeruginosa
… by 11 bp, within the promoter region of pyrR. Gel retardation assays using upstream DNA fragments proved PyrR binds to the DNA of pyrD, pyrE, pyrF as well as pyrR. This suggests that expression of pyrR is autoregulated; moreover, a stable stem-loop structure was determined in the pyrR promoter …
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Photo-responsive degradable poly (beta-amino ester) toward non-viral gene delivery
… of UV-responsive gene delivery properties. Gel permeation chromatography (GPC) and UV-vis analyses confirmed that UV irradiation triggered degradation of the responsive polymer but not the control polymer. The cationic PBAE condenses DNA into 100-nm complexes due to electrostatic …
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Function of the forkhead gene fd3F in Drosophila chordotonal neuron differentiation
… and inactive using GFP enhancer constructs and gel retardation assays. I therefore hypothesise that fd3F is an important component of the gene regulatory network that links atonal expression in SOPs to differentiation of Ch organs. In particular fd3F regulates genes required specifically for Ch …
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The role of steroidogenic factor-1 (SF-1) in gonadotropin-releasing hormone (GnRH) receptor gene regulation
… in the gonadotrope cell lines, αT3-1 and αT4, by gel retardation assays. DNasel footprinting reveals that the major DNA-binding activity in αT3-1 cells on the GnRHR promoter occurs at the SF-1-like site. The SF-1-like sequence specificity of the interaction is demonstrated by gel redardation and …
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Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery
… by RP–HPLC and verified by polyacrylamide gel electrophoresis. To determine the biodistribution and pharmacokinetic parameters of Gal–PEG–ODN, ODN was radiolabeled by <sup>33</sup>P before the conjugation reaction. Following tail vein injection into rats, Gal–PEG–<sup>33</sup>P–ODN rapidly …
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The molecular biology of calbindin-D₉
… bind the estrogen receptor was investigated by gel retardation assay. No binding was detected when the human and porcine sequences were used. The inability of the human sequence to bind the estrogen receptor may explain why the gene is not expressed in human uterus (and placenta). In the case of …
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In vivo and in vitro regulation of plasminogen activator inhibitor type-1
… The active regions were also analyzed by gel retardation to investigate the transcription factors that bound to these regions. The functional data taken together with the DNA binding data supported the hypothesis that the response of the PAl-1 promoter to TGFP was mediated by two conserved …
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Zur Struktur und Funktion regulatorischer Elemente des cbb-Regulons in Ralstonia eutropha
… der cbb-Regulation involvierten Regulators hin. Gelretardationsexperimente mit der cbb-Kontrollregion führten zur Detektion zweier Proteine, P1 und P2, deren neben CbbR ebenfalls spezifische Bindung an die cbb-Kontrollregion nachgewiesen wurde. P2 wurde in der Folge als ein Ortholog von PhcA aus …